Wasp的尾巴与Cell Migration有关
How Cdc42 and acidic
phospholipids might activate WASP. Inset shows an actin-filament
halo (red) surrounding a vesicle (green). Photo courtesy of Henry Higgs and Tom Pollard, Salk Institute, La Jolla,California, USA.
细胞的移动需要肌动蛋白的多聚化，外界的刺激究竟是如何促使细胞发生改变的呢?有证据表明WASP(Wiskott–Aldrich
syndrome protein)家族蛋白可能是信号传递者，但对于科学家来说，细胞移动的详细机制始终难以捉摸。The
Journal of Cell Biology上的两篇文章指出脂类与蛋白质相互联合并激活了WASP。
肌动蛋白的成核现象有Arp2/3复合物激活，Arp2/3是由WASP活化的。一种小GTPase
Cdc42及一种磷脂PtdIns(4,5)P2活化了WASP。
Henry
Higgs and Tom Pollard从小牛胸腺中纯化了haematopoietic-cell特异性的WASP，他们发现纯化的WASP是无活性的，但当包含了PtdIns(4,5)P2的微团，cdc42或包含另一种磷脂，磷脂酰丝氨酸的小泡存在时，WASP能将肌动蛋白多聚化了。此过程需要的cdc42是结合了GTP，被活化的。
Rohatgi and colleagues研究了另一种广泛表达的N－WASP的重组蛋白，发现cdc42而不是PtdIns(4,5)P2能够部分激活N-WASP，而两者联合能完全激活N-WASP。他们对WASP的C末端进行了分析，发现了如图的精巧机制，解释了WASP的自抑制机制，也解释了cdc42和磷脂对WASP的激活机制。
相关文章：
ORIGINAL RESEARCH PAPER
Higgs , H. N. & Pollard,T. D. Activation by Cdc42 and PIP2 of Wiskott–Aldrich syndrome
protein (WASP) stimulates actin nucleation by Arp2/3 complex. J. Cell
Biol. 150, 1311–1320 (2000). [ PubMed] PubMed
Rohatgi, R. et al. Mechanism
of N-WASP activation by CDC42 and Phosphatidylinositol
4,5-bisphosphate. J. Cell Biol. 150, 1299 –1309 (2000). [ PubMed]
PubMed
FURTHER READING
Cameron, L. A. et al. Secrets
of actin-based motility revealed by a bacterial pathogen. Nature Rev.
Mol. Cell Biol. 1, 110–119 (2000) [At a glance]
Kim, A. S., Kakalis, L.T.,Abdul-Manan, N., Liu, G.A. & Rosen, M.K. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature
404, 151- 158 (2000). [PubMed] PubMed
Blanchoin, L. et al. Direct
observation of dendritic actin filament networks nucleated by Arp2/3
complex and WASP/Scar proteins. Nature 404 , 1007-1011 (2000). [PubMed]
PubMed, http://www.100md.com
phospholipids might activate WASP. Inset shows an actin-filament
halo (red) surrounding a vesicle (green). Photo courtesy of Henry Higgs and Tom Pollard, Salk Institute, La Jolla,California, USA.
细胞的移动需要肌动蛋白的多聚化，外界的刺激究竟是如何促使细胞发生改变的呢?有证据表明WASP(Wiskott–Aldrich
syndrome protein)家族蛋白可能是信号传递者，但对于科学家来说，细胞移动的详细机制始终难以捉摸。The
Journal of Cell Biology上的两篇文章指出脂类与蛋白质相互联合并激活了WASP。
肌动蛋白的成核现象有Arp2/3复合物激活，Arp2/3是由WASP活化的。一种小GTPase
Cdc42及一种磷脂PtdIns(4,5)P2活化了WASP。
Henry
Higgs and Tom Pollard从小牛胸腺中纯化了haematopoietic-cell特异性的WASP，他们发现纯化的WASP是无活性的，但当包含了PtdIns(4,5)P2的微团，cdc42或包含另一种磷脂，磷脂酰丝氨酸的小泡存在时，WASP能将肌动蛋白多聚化了。此过程需要的cdc42是结合了GTP，被活化的。
Rohatgi and colleagues研究了另一种广泛表达的N－WASP的重组蛋白，发现cdc42而不是PtdIns(4,5)P2能够部分激活N-WASP，而两者联合能完全激活N-WASP。他们对WASP的C末端进行了分析，发现了如图的精巧机制，解释了WASP的自抑制机制，也解释了cdc42和磷脂对WASP的激活机制。
相关文章：
ORIGINAL RESEARCH PAPER
Higgs , H. N. & Pollard,T. D. Activation by Cdc42 and PIP2 of Wiskott–Aldrich syndrome
protein (WASP) stimulates actin nucleation by Arp2/3 complex. J. Cell
Biol. 150, 1311–1320 (2000). [ PubMed] PubMed
Rohatgi, R. et al. Mechanism
of N-WASP activation by CDC42 and Phosphatidylinositol
4,5-bisphosphate. J. Cell Biol. 150, 1299 –1309 (2000). [ PubMed]
PubMed
FURTHER READING
Cameron, L. A. et al. Secrets
of actin-based motility revealed by a bacterial pathogen. Nature Rev.
Mol. Cell Biol. 1, 110–119 (2000) [At a glance]
Kim, A. S., Kakalis, L.T.,Abdul-Manan, N., Liu, G.A. & Rosen, M.K. Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature
404, 151- 158 (2000). [PubMed] PubMed
Blanchoin, L. et al. Direct
observation of dendritic actin filament networks nucleated by Arp2/3
complex and WASP/Scar proteins. Nature 404 , 1007-1011 (2000). [PubMed]
PubMed, http://www.100md.com