TNF-α对胃癌细胞MMP-2和MMP-9表达的诱导作用
胃癌;肿瘤坏死因子α;基质金属蛋白酶2;基质金属蛋白酶9;酶链免疫吸附试验;逆转录-聚合酶链反应;蛋白印迹,王峻,郭德玉,王峻,通讯作者:,Inductionofmatrixmetalloproteinase-2andmatrixme
 |
| 第1页 |
参见附件(880KB，5页)。
王峻, 郭德玉, 中国人民解放军第三军医大学附属西南医院病理科 重庆市 400038
王峻, 2003级在读硕士研究生, 主要从事肿瘤免疫学方面的研究.
通讯作者: 郭德玉, 400038, 重庆市, 中国人民解放军第三军医大学附属西南医院病理科. gdy007@mail.tmmu.com.cn
电话: 023-68765455
收稿日期: 2007-01-09 接受日期: 2007-01-27
Induction of matrix metalloproteinase-2 and matrix metalloproteinase-9 expression in gastric cancer cells by tumor necrosis factor-α
Jun Wang, De-Yu Guo
Jun Wang, De-Yu Guo,Department of Pathology, Southwest Hospital Affiliated to the Third Military Medical University, Chongqing 400038, China
Correspondence to:De-Yu Guo, Department of Pathology, Southwest Hospital Affiliated to the Third Military Medical University, Chongqing 400038, China. gdy007@mail.tmmu.com.cn
Received:2007-01-09 Accepted:2007-01-27
Abstract
AIM: To investigate the inductive effect of tumor necrosis factor-α (TNF-α) on the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in gastric cancer cells.
METHODS: Gastric cancer cells were stimulated by exogenous TNF-α and the cells without stimulation served as controls. The contents of MMP-2 and MMP-9 in the supernatant fluid of gastric cancer cells were measured by enzyme-linked immunosorbnent assay (ELISA). The expression of MMP-2 and MMP-9 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Western blot was used to detect the expression of MMP-2 and MMP-9 protein in gastric cancer cells.
RESULTS: Following stimulation with 1, 10 and 100 mg/L TNF-α, the contents of MMP-2 (8.24 ± 1.36, 23.65 ± 3.45, 14.83 ± 2.54 ng/L) and MMP-9 (12.47 ± 2.66, 34.12 ± 6.78, 23.31 ± 4.45 ng/L) in the supernatant fluid of gastric cancer cells were increased significantly as compared with those in the control group (MMP-2: 1.56 ± 0.23 ng/L; 5.25 ± 0.89 ng/L; all P < 0.01). After 16 hours of stimulation with TNF-α, the contents of MMP-2 and MMP-9 in the supernatant fluid reached the peak values, which were 23 ......
您现在查看是摘要介绍页，详见PDF附件(880KB，5页)。