碘克沙醇及Ficoll-400分离纯化大鼠胰岛细胞的效果对比
碘克沙醇;Ficoll;胰岛细胞;纯化,张桦,陈宏,张振,杨力,孙嘉,李明,刘宏,蔡德鸿,通讯作者:,EfficacycomparisonofratisletpurificationbetweeniodixanolandFicolld
 |
| 第1页 |
参见附件(906KB，4页)。
张桦, 陈宏, 张振, 杨力, 孙嘉, 李明, 刘宏, 蔡德鸿, 南方医科大学附属珠江医院内分泌科 广东省广州市 510282
国家自然科学基金资助项目, No. 30470829
通讯作者: 蔡德鸿, 510282, 广东省广州市工业大道中253号, 南方医科大学附属珠江医院内分泌科. jimzhua@163.com
电话: 020-61643181 传真: 020-84309080
收稿日期: 2007-03-13 接受日期: 2007-03-31
Efficacy comparison of rat islet purification between iodixanol and Ficoll density gradient method
Hua Zhang, Hong Chen, Zhen Zhang, Li Yang, Jia Sun, Ming Li, Hong Liu, De-Hong Cai
Hua Zhang, Hong Chen, Zhen Zhang, Li Yang, Jia Sun, Ming Li, Hong Liu, De-Hong Cai,Department of Endocrinology, Zhujiang Hospital Affiliated to Nanfang Medical University, Guangzhou 510282, Guangdong Province, China
Supported byNational Natural Science Foundation of China, No. 30470829
Correspondence to:De-Hong Cai, Departments of Endocrinology, Zhujiang Hospital Affiliated to Nanfang Medical University, 253 Gongye Middle Avenue, Guangzhou 510282, Guangdong Province, China. jimzhua@126.com
Received:2007-03-13 Accepted:2007-03-31
Abstract
AIM: To compare the yield, purity, quality and function of rat islets after purification by iodixanol or Ficoll-400 density gradient method.
METHODS: Twenty Sprague Dawley rats were randomly divided into iodixanol and Ficoll group according to the corresponding purification methods. Rat pancreas was isolated by collagenase P digestion, and the rat islets of two groups were purified by iodixanol or Ficoll-400 density gradient method. After isolation, the islet yield and purity were evaluated by DTZ staining and the islet function was assessed by insulin release assays in vitro and islet allotransplantation in vivo.
RESULTS: There were no marked differences in the yield and purity of islets between both groups. The survival rate of islets in iodixanol group was significantly higher than that in Ficoll group (93.3% ± 3.5%vs 84.8% ± 3.8%, P < 0.01), and the time of hyperglycemia reversal in diabetic rat was also longer (11 ......
您现在查看是摘要介绍页，详见PDF附件(906KB，4页)。