三氧化二砷诱导胰腺癌细胞系PC-3凋亡及其抑制转移的作用
三氧化二砷;胰腺癌细胞;凋亡;转移,刘静冰,秦叔逵,李进,通讯作者:,EffectsofarsenictrioxideonthepancreaticcarcinomacelllinePC-3andinhibitorymigration
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刘静冰, 秦叔逵, 中国人民解放军第八一医院全军肿瘤中心 江苏省南京市 210002
李进, 上海复旦大学医学院附属肿瘤医院 上海市 200032
南京军区医学科研“十·五”计划课题, No. 02MA014
通讯作者: 秦叔逵, 210002, 江苏省南京市杨公井34标34号，中国人民解放军第八一医院全军肿瘤中心肿瘤内科. qinsk@csco.org.cn
电话: 025-84453932 传真: 025-84453932
收稿日期: 2007-02-25 接受日期: 2007-03-23
Effects of arsenic trioxide on the pancreatic carcinoma cell line PC-3 and inhibitory migration
Jing-Bing Liu, Shu-Kui Qin, Jin Li
Jing-Bing Liu, Shu-Kui Qin, Oncology Center of Chinese PLA 81st Hospital, Nanjing 210002, Jiangsu Province, China
Jin Li, Cancer Hospital, Medical Center of Fudan University, Shanghai 200032, China
Supported by: the Medical Research Program of Nanjing Military Region during the 10th Five-Year Plan Period, No. 02MA014
Correspondence to: Shu-Kui Qin, Oncology Center of Chinese PLA 81st Hospital, No. 34, 34 Biao, Yanggongjing Street, Nanjing 210002, Jiangsu Province, China. qinsk@hotmail.com
Received: 2007-02-25 Accepted: 2007-03-23
Abstract
AIM: To explore the effects of As2O3 on apoptosis and its inhibitory migration in a human pancreatic carcinoma cell line.
METHODS: The human pancreatic carcinoma PC-3 cell line was treated with As2O3. The rate of apoptosis and the growth cycle of the cell line were investigated by flow cytometry. The expression of gene proteins related to apoptosis (Fas, Fas-L, Bcl-2, and Bax) and metastasis (CD44 and nm23) were observed by immunohistochemistry.
RESULTS: A visible apoptotic peak and blockage of the S phase of the cell cycle (14.86%-63.66%) were shown by flow cytometry. Immunohistochemical staining indicated that the expressions of Bcl-2 (+++~+) and CD44 (++++~+) were down regulated, while the expressions of Fas (+~+++), Fas-L (-~+++) and nm23 (+~+++) were up-regulated.
CONCLUSION: Injections of As2O3 can induce human pancreatic carcinoma cell apoptosis and inhibit pancreatic carcinoma migration ......
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