内毒素调节肝脏β-葡萄糖苷酸酶mRNA的表达
内毒素;胆石症;β-葡萄糖苷酸酶;发病机制;逆转录聚合酶链反应,孙韶龙,吴硕东,戴显伟,崔东旭,隋东明,孙韶龙,通讯作者:,Endotoxinregulatestheexpressionofhepatocyteβ-glucuronidasemRNA
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孙韶龙, 吴硕东, 戴显伟, 崔东旭, 中国医科大学附属盛京医院普外科 辽宁省沈阳市 110004
隋东明, 盘锦市中医院外科 辽宁省盘锦市 124000
孙韶龙, 讲师, 主要从事肝胆疾病的研究.
通讯作者: 孙韶龙, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学盛京医院普外科. doublegragons@yahoo.com.cn
电话: 024-83956436 传真: 024-86540367
收稿日期: 2007-01-15 接受日期: 2007-04-21
Endotoxin regulates the expression of hepatocyte β-glucuronidase mRNA
Shao-Long Sun, Shuo-Dong Wu, Xian-Wei Dai, Dong-Ming Sui, Dong-Xu Cui
Shao-Long Sun, Shuo-Dong Wu, Xian-Wei Dai, Dong-Xu Cui, Department of General Surgery, the Second Affiliated Hospital (Shengjing Hospital) of China Medical University, Shenyang 110004, Liaoning Province, China
Dong-Ming Sui, Department of Surgery, Panjin Traditional Chinese Medical Hospital, Panjin 124000, Liaoning Province, China
Correspondence to: Dr. Shao-Long Sun, Department of General Surgery, the Second Affiliated Hospital (Shengjing Hospital) of China Medical University, Shenyang, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. doublegragons@yahoo.com.cn
Received: 2007-01-15 Accepted: 2007-04-21
Abstract
AIM: To investigate the effect of endotoxin on the expression of hepatocyte GUSB mRNA and serum β-glucuronidase (β-GD) activity in mice.
METHODS: Twenty-three BALB/c mice were divided into two groups: control (injection of Saline without endotoxin) (n = 5) and LPS (n = 18). In the Lipopolysa cc haride (LPS) group, after injection of endotoxin, mice were put to death at 1/2, 2, 4, and 24 hours by breaking cervical vertebra. Serum β-glucuronidase activity was detected by an enzyme kinetics method; and the expression of GUSB mRNA in hepatocytes was detected by semi-quantity reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: In the LPS 1/2, 2 and 4 h groups, the activity of β-GD levels (3.96 ± 0.88, 4.40 ± 0.55, 5.65 ± 0.58 Fishman Unit, respectively) was significantly higher than that of the controls (2 ......
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