T淋巴细胞亚群和DNA倍体检测在腹水鉴别诊断中的价值
T淋巴细胞亚群;DNA倍体;腹水;流式细胞;鉴别诊断,张卫国,童强,王强,王小虎,李胜保,通讯作者:,ValueofTlymphocytesubsetdeterminationandDNAploidyanalysisfordifferentia
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张卫国, 童强, 王强, 王小虎, 李胜保, 郧阳医学院附属太和医院消化内科 湖北省十堰市 442000
通讯作者: 童强, 442000, 湖北省十堰市, 郧阳医学院附属太和医院消化内科. zwg789@sina.com
电话: 0719-8801430
收稿日期: 2006-12-20 接受日期: 2007-04-02
Value of T lymphocyte subset determination and DNA ploidy analysis for differential diagnosis of ascites
Wei-Guo Zhang, Qiang Tong, Qiang Wang, Xiao-Hu Wang, Sheng-Bao Li
Wei-Guo Zhang, Qiang Tong, Qiang Wang, Xiao-Hu Wang, Sheng-Bao Li, Department of Gastroenterology, the Affiliated Taihe Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China
Correspondence to: Wei-Guo Zhang, Department of Gastroenterology, the Affiliated Taihe Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China. zwg789@sina.com
Received: 2006-12-20 Accepted: 2007-04-02
Abstract
AIM: To investigate the value of T lymphocyte subset determination and DNA ploidy analysis for differential diagnosis of ascites.
METHODS: In 74 cases of ascites, 24 with tuberculous peritonitis, 21 with liver cirrhosis and 29 with carcinomatous ascites, T lymphocyte subsets and DNA ploidy in ascitic fluid were detected by flow cytometry.
RESULTS: In descending order of T lymphocyte (CD3+), helper/inducer T lymphocyte (CD4+), and helper T lymphocyte/suppressor T lymphocyte (CD4+/CD8+) in ascitic fluid, tuberculous peritonitis (CD3+: 86.2% ± 5.1%, CD4+: 64.3% ± 6.4%, CD4+/CD8+: 3.20% ± 0.30%), carcinomatous ascites (65.7% ± 4.6%, 32.5% ± 2.2%, 1.04 % ± 0.11%) and liver cirrhosis (15.1% ± 2.7%, 3.6% ± 0.5%, 0.36% ± 0.05%) (P < 0.01) were determined. In ascending order, CD8+, liver cirrhosis (10.1% ± 3.2%), tuberculous peritonitis (20.1% ± 4.3%) and carcinomatous ascites (31.3% ± 5.2%) (P < 0.01) were determined. The positive rate from DNA ploidy analysis in carcinomatous ascites (89 ......
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