外源性导入抑癌基因FHIT对肝癌细胞Hep3B生长的影响
肝癌;基因治疗;脆性组氨酸三联体,许荣华,郑武平,孟津,夏立平,易继林,许荣华,通讯作者:,EffectsoffragilehistidinetriadgenetransductionongrowthofHep3Bhumanhep
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许荣华, 郑武平, 孟津, 夏立平, 海南医学院附属医院肿瘤外科 海南省海口市 570102
易继林, 华中科技大学同济医学院附属同济医院普外科 湖北省武汉市 430030
许荣华, 医学博士, 讲师, 主治医师, 主要从事肝脏肿瘤的基础与临床研究.
通讯作者: 许荣华, 570102, 海南省海口市, 海南医学院附属医院肿瘤外科. xu_ronghua2231@hotmail.com
电话: 0898-66528115
收稿日期: 2007-04-02 接受日期: 2007-04-28
Effects of fragile histidine triad gene transduction on growth of Hep3B human hepatocellular carcinoma cells
Rong-Hua Xu, Wu-Ping Zheng, Jin Meng, Li-Ping Xia, Ji-Lin Yi
Rong-Hua Xu, Wu-Ping Zheng, Jin Meng, Li-Ping Xia,Department of Oncology Surgery, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China
Ji-Lin Yi,Department of General Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to:Rong-Hua Xu, Department of Oncology Surgery, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China. xu_ronghua2231@hotmail.com
Received:2007-04-02 Accepted:2007-04-28
Abstract
AIM: To evaluate the effects of the human fragile histidine triad gene on cell proliferation and apoptosis of the Hep3B human hepatocellular carcinoma line.
METHODS: A recombinant pcDNA3.1 (+)/FHIT including functional region of human fragile histidine triad gene (FHIT) was constructed for transfer into human hepatocellular carcinoma cells in vitro. mRNA and protein expressions of the gene in the transfected cells were detected by reverse transcriptase-polymerase chain reaction and Western blot, respectively. MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay was used to assess the effects of FHIT on proliferation. Cell cycle changes and apoptosis were measured by flow cytometry.
RESULTS: Hep3B cells expressed high levels of FHIT-mRNA and FHIT protein after infection with pcDNA3.1 (+)/FHIT. The growth of Hep3B cells treated with pcDNA3 ......
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