人胃癌顺铂耐药细胞系的建立过程
胃癌;SGC7901,DDP;顺铂;药物耐受性,潘洪明,费洪新,杜静平,陈正华,张涛,潘洪明,通讯作者:,Constructionofacisplatin-inducedhumangastriccancerdrug-resistantcelllin
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潘洪明, 齐齐哈尔医学院生物化学教研室 黑龙江省齐齐哈尔市 161042
费洪新, 齐齐哈尔医学院组织学与胚胎学教研室 黑龙江省齐齐哈尔市 161042
杜静平, 陈正华, 亚盛集团博士后科研工作站 北京市 100101
张涛, 佳木斯大学生物化学教研室 黑龙江省佳木斯市 154007
潘洪明, 副教授, 硕士生导师, 主要研究中药多糖抗肿瘤机制.
通讯作者: 潘洪明, 161042, 齐齐哈尔市富拉尔基区, 齐齐哈尔医学院生物化学教研室. qyphm@sohu.com
电话: 0452-6731101
收稿日期: 2007-04-13 接受日期: 2007-05-22
Construction of a cisplatin-induced human gastric cancer drug-resistant cell line
Hong-Ming Pan, Hong-Xin Fei, Jing-Ping Du, Zheng-Hua Chen, Tao Zhang
Hong-Ming Pan,Department of Biochemistry, Qiqihaer Medical College, Qiqihaer 161042, Heilongjiang Province, China
Hong-Xin Fei,Department of Histology and Embryology; Qiqihaer Medical College, Qiqihaer 161042, Heilongjiang Province, China
Jing-Ping Du, Zheng-Hua Chen,Postdoc Workstation of Gansu Yasheng Industrial Co. Beijing 100101, China
Tao Zhang, Department of Biochemistry, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
Correspondence to:Hong-Ming Pan, Department of Biochemistry, Qiqihaer Medical College, Qiqihaer Fulaerji District, Qiqihaer 161042, Heilongjiang Province, China. qyphm@sohu.com
Received:2007-04-13 Accepted:2007-05-22
Abstract
AIM: To establish a cisplatin-induced human gastric cancer drug-resistant cell line and study changes in its resistance.
METHODS: A resistant gastric cancer cell line (SGC7901/DDP) was established by gradually increasing the dose of cisplatin with intermittent, monthly administration. Drug sensitivity was detected by MTT assay. Changes in biological characteristics were determined using light microscopy, electron microscopy, cell counting by MTT assay, and flow cytometry.
RESULTS: The SGC7901/DDP cell line was developed after 4 mo, with stable resistance to cisplatin. The resistance index for each month was 2.13, 5.32, 12.60 and 12.93, respectively. SGC7901/DDP cells exhibited cross-resistance to many other chemotherapeutic agents (e ......
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