共轭三烯酸抑制肝癌细胞体外增殖和凋亡诱导的作用

http://www.100md.com 2007年7月8日 吴亚英, 王修杰, 林 苹, 张 洁, 任婧婧, 杨洪亮, 高艳萍, 刘 凯

关键词：共轭三烯酸;共轭亚油酸;肝癌细胞;增殖抑制;凋亡;MTT法;流式细胞术,吴亚英,王修杰,林苹,张洁,任婧婧,杨洪亮,高艳萍,刘凯,通讯作者:,Proliferationinhibitionandapoptosisinductionofconjugat

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     吴亚英, 王修杰, 林苹, 张洁, 任婧婧, 杨洪亮, 高艳萍, 刘凯, 四川大学华西医院生物治疗国家重点实验室 实验肿瘤研究室 四川省成都市 610041

    吴亚英, 四川大学华西医学中心临床医学院硕士, 主要从事肿瘤学研究.

    通讯作者: 王修杰, 610041, 四川省成都市, 四川大学华西医院生物治疗国家重点实验室, 实验肿瘤学研究室.

    xiujiewang@sina.com

    电话: 028-85164018

    收稿日期: 2007-04-28 接受日期: 2007-05-22

    Proliferation inhibition and apoptosis induction of conjugated trienoic fatty acids in hepatocellular carcinoma cells

    Ya-Ying Wu, Xiu-Jie Wang, Ping Lin, Jie Zhang, Jing-Jing Ren, Hong-Liang Yang, Yan-Ping Gao, Kai Liu

    Ya-Ying Wu, Xiu-Jie Wang, Ping Lin, Jie Zhang, Jing-Jing Ren, Hong-Liang Yang, Yan-Ping Gao, Kai Liu, Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, Huaxi Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China

    Correspondence to: Xiu-Jie Wang, 610041, Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, Huaxi Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China. xiujiewang@sina.com

    Received: 2007-04-28 Accepted: 2007-05-22

    Abstract

    AIM: To investigate the effects of conjugated trienoic fatty acids on the proliferation of hepatocellular carcinoma cells and to elucidate the mechanism of their activity.

    METHODS: Normal liver, hepatocellular carcinoma SMMC-7721 and hepa1–6 cells were tested in vitro for cytotoxicity, colony formation inhibition, BrdU incorporation, Hoechst 33342 apoptotic cell staining, and cell cycle distribution, through flow cytometry (FCM) after treatment with conjugated trienoic fatty acids.

    RESULTS: After conjugated trienoic fatty acid treatment, proliferation, colony formation and BrdU labeling indices of cancer cells decreased (proliferation: SMMC-7721, 1.5 ± 2.6 vs 1.9 ± 12.3, P < 0.05; Hepa1-6, 1.0 ± 1.5 vs 1.2 ± 9.7, P < 0.05; colony formation: SMMC-7721, 149.3 ± 3.1 vs 191.7 ± 5.8,P < 0.05; Hepa1-6, 32.7 ± 3.1 vs 61.3 ± 4.2, P < 0.05; BrdU labeling indices: SMMC-7721, 42 ......

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