5-Aza-CdR体外诱导胃癌细胞系p16基因去甲基化及表达增强
5-Aza-CdR;p16基因;胃癌;DNA甲基化,沈文静,戴冬秋,滕玥,刘洁,通讯作者:,5-aza-2'-deoxycytidineinducesdemethylationofp16geneinhumangastriccan
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沈文静, 中国医科大学附属第一医院妇科 辽宁省沈阳市 110001
戴冬秋, 滕玥, 中国医科大学附属第一医院肿瘤外科 辽宁省沈阳市 110001
刘洁, 中国医科大学实验技术中心二部 辽宁省沈阳市 110001
沈文静, 博士生, 研究方向胃癌、卵巢癌转移分子机制与临床应用.
国家自然科学基金资助项目, No. 30572162, No. 30271477; 教育部高校博士点基金资助项目, No. 20050159001
通讯作者: 戴冬秋, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤外科. daidq63@163.com
电话: 024-83283560
收稿日期: 2007-02-16 接受日期: 2007-05-16
5-aza-2'-deoxycytidine induces demethylation of p16 gene in human gastric cancer cells and up-regulates its expression
Wen-Jing Shen, Dong-Qiu Dai, Yue Teng, Jie Liu
Wen-Jing Shen, Department of Gynecology, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China
Dong-Qiu Dai, Yue Teng, Department of Surgical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China
Jie Liu, The second section Laboratory Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China
Supported by: National Natural Science Foundation of China, No. 30572162, No. 30271477; and the Scientific Research Foundation of Education Ministry for the Doctors, No. 20050159001
Correspondence to: Dr. Dong-Qiu Dai, Department of Surgical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China. daidq63@163.com
Received: 2007-02-16 Accepted: 2007-05-16
Abstract
AIM: To investigate the effects of 5-aza-2'-deoxycytidine (5-Aza-CdR) on methylation and expression of p16 gene in the human gastric cancer cell line SGC7901, and to discuss the mechanism of p16 gene silencing in human gastric cancer cells, as well as the regulating effect of the demethylating agent on p16 gene expression.
METHODS: SGC7901 cells were cultured in RPMI 1640 medium and were treated with different concentrations (1 × 10–7 mol/L, 5 × 10–7 mol/L, 1 × 10–6 mol/L, 5 × 10–6 mol/L) of DNA methyltransferase inhibitor 5-Aza-CdR ......
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