外源性一氧化碳对小肠缺血再灌注大鼠不同器官超微结构及细胞凋亡的影响
一氧化碳;小肠;缺血再灌注;超微结构;凋亡;TUNEL法,韩宁,陈卫民,韩宁,通讯作者:,Effectsofexogenouscarbonmonoxideonultrastructurechangesandapoptosisof
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韩宁, 陈卫民, 中国医科大学附属盛京医院麻醉科 辽宁省沈阳市 110004
韩宁, 2007年中国医科大学博士研究生毕业, 讲师/主治医师, 主要从事临床麻醉, 危重症监护治疗和疼痛治疗等方面的研究.
通讯作者: 韩宁, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学附属盛京医院麻醉科. hann@cmu2h.com
电话: 024-83955027
收稿日期: 2007-05-11 修回日期: 2007-09-28
Effects of exogenous carbon monoxide on ultrastructure changes and apoptosis of different organs induced by intestinal ischemia/reperfusion in rats
Ning Han, Wei-Min Chen
Ning Han, Wei-Min Chen, Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Ning Han, Department of Anesthesiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. hann@cmu2h.com
Received: 2007-05-11 Revised: 2007-09-28
Abstract
AIM: To investigate the effects of CO on multiple organ injury induced by intestinal ischemia/reperfusion (IIR) in rats.
METHODS: Sixty-four male Wistar rats, weighing 220–260 g, were randomly allocated into eight groups with eight animals in each. The model of IIR was established by clamping the superior mesenteric artery (SMA) for 60 min and reperfusion for 120 min. Group A, sham operation, no SMA clamping; group B, SMA clamping for 60 min and reperfusion for 120 min; group C1/C2, inhaled 100 µL/L/250 µL/L CO 10 min before SMA clamping; group D1/D2, inhaled 100 µL/L/250 µL/L CO 60 min after SMA clamping; group E1/E2, inhaled 100 µL/L/250 µL/L CO 60 min after reperfusion. The ultrastructure of different tissues was observed by transmission electron microscopy, and apoptosis of different cells was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL).
RESULTS: The extent of ultrastructural damage in the different tissues in group B was intestinal > lung > liver, with no significant change in the kidneys; and in different groups, it was group C < D < E < B, with no significant differences between the 250 µL/L and 100 µL/L groups ......
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