全反式维甲酸对酒精性肝损伤大鼠肝脏retinoids含量和CYP2E1表达的影响

http://www.100md.com 2007年11月8日 潘志红, 但自力, 龚发良, 郑宗军

鍏抽敭璇嶏細酒精性肝病;全反式维甲酸;Retinoids;CYP2E1;高效液相色谱;免疫印迹,潘志红,龚发良,郑宗军,但自力,通讯作者:,Effectofall-transretinoicacidonretinoidconc

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     潘志红, 龚发良, 郑宗军, 宜昌市第一人民医院消化内科 湖北省宜昌市 443000

    但自力, 华中科技大学同济医学院附属同济医院消化内科 湖北省武汉市 430030

    潘志红, 硕士, 主要从事肝脏疾病的研究.

    通讯作者: 但自力, 430030, 湖北省武汉市, 武汉市汉口解放大道1095号, 华中科技大学同济医学院附属同济医院消化内科. danzili@msn.com

    电话: 027-83663611

    收稿日期: 2007-06-26 修回日期: 2007-09-24

    Effect of all-trans retinoic acid on retinoid concentrations and expression of CYP2E1 in the livers of ethanol-fed rats

    Zhi-Hong Pan, Zi-Li Dan, Fa-Liang Gong, Zong-Jun Zheng

    Zhi-Hong Pan, Fa-Liang Gong, Zong-Jun Zheng, Department of Gastroenterology, Yichang First People’s Hospital, Yichang 443000, Hubei Province, China

    Zi-Li Dan, Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China

    Correspondence to: Professor Zi-Li Dan, Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, Hubei Province, China. danzili@msn.com

    Received: 2007-06-26 Revised: 2007-09-24

    Abstract

    AIM: To investigate the effects of low-dose all-trans-retinoic acid (ATRA) supplementation on the concentrations of hepatic retinoids, formation of polar retinoid metabolites (PRMs), expression of microsomal cytochrome P4502E1 (CYP2E1) and liver injury in ethanol-fed rats.

    METHODS: A rat model of alcoholic liver disease (ALD) was induced by intragastric infusion of ethanol. Thirty male SD rats were randomly assigned to four groups: group A was a control group, rats of groups B, C and D were received intra-gastric infusion of alcohol (8 g/kg) for 8 weeks, and rats of groups C and D were administered all-trans-RA at two different doses (150 µg/kg body wt in group C and 1.5 mg/kg body wt in group D), by intragastric infusion, for 4 weeks. The concentrations of retinoids in rat liver were detected using high-performance liquid chromatography (HPLC), the levels of CYP2E1 were measured by Western blotting, and pathological changes in the livers were observed under light microscope ......

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