黏附分子在沙利度胺所致大鼠肝纤维化中的作用机制
肝纤维化;沙利度胺;细胞黏附分子,肖艳菊,罗和生,吕鹏,肖艳菊,通讯作者:,Effectsofthalidomideonadhesionmoleculesinratliverfibrosis,Yan-JuXiao,He-ShengLuo,Peng
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肖艳菊, 罗和生, 吕鹏, 武汉大学人民医院消化内科 湖北省武汉市 430060
肖艳菊, 硕士, 主要从事消化道疾病的基础与临床的研究.
通讯作者: 罗和生, 430060, 湖北省武汉市, 武汉大学人民医院消化内科. luotang@public.wh.hb.cn
收稿日期: 2007-06-27 修回日期: 2007-10-18
Effects of thalidomide on adhesion molecules in rat liver fibrosis
Yan-Jiu Xiao, He-Sheng Luo, Peng Lv
Yan-Ju Xiao, He-Sheng Luo, Peng Lv, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to: He-Sheng Luo, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. luotang@public.wh.hb.cn
Received: 2007-06-27 Revised: 2007-10-18
Abstract
AIM: To investigate the therapeutic effects and mechanism of thalidomide on hepatic fibrosis in rats.
METHODS: Liver fibrosis in rats was induced by intraperitoneal injection of carbon tetrachloride for 4 weeks. The rats were killed at 2, 4 and 6 wk after treatment with 100 mg/kg intragastric thalidomide. Liver fibrosis score was assessed under the light microscope by H&E staining. Serum hyaluronic acid (HA), laminin (LN), procollagen type Ⅲ (PCⅢ) and collagen type Ⅳ were detected by radioimmunoassay. Expression of ICAM-1, VCAM-1 and E selectin were examined by immunohistochemistry. mRNA levels for ICAM-1, VCAM-1, and E selectin was detected by reverse-transcriptase polymerase chain reaction.
RESULTS: Compared with the liver fibrosis group, fibrosis score in thalidomide-treated rats (4 or 6 wk) was obviously improved (P < 0.05). Serum HA (176.6 ± 7.5 μg/L, 173.8 ± 6.7 μg/L vs 486.9 ± 12.4 μg/L), LN (38.4 ± 5.8 μg/L, 34.7 ± 7.3 μg/L vs 84.5 ± 5.2 μg/L), PCⅢ (44.3 ± 5.5 μg/L, 40.2 ± 6.1 μg/L vs 65.0 ± 5.6 μg/L) and collagen Ⅳ (31.8 ± 6.7 μg/L, 30.4 ± 5.7 μg/L vs 55.6 ± 6.5 μg/L) in rats treated with thalidomide for 4 or 6 wk were significantly lower than those in rats with liver fibrosis (P < 0.05) ......
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