hAFP增强子调控的肝癌特异性基因治疗载体的构建及活性测定
http://www.100md.com
马晓娟, 匡安仁, 黄 蕤
甲胎蛋白;启动子;增强子;克隆;聚合酶链式反应;载体;肝癌,马晓娟,匡安仁,黄蕤,马晓娟,通讯作者:,Constructionofthealpha-fetoproteinenhancercontrolledgenetherapyvect
 |
| 第1页 |
参见附件(589KB，5页)。
马晓娟, 匡安仁, 黄蕤, 四川大学华西医院核医学科 四川省成都市 610041
马晓娟, 1997年东南大学医学院本科, 主治医师, 主要从事分子核医学研究工作.
国家自然科学基金资助项目, No. 30670585
通讯作者: 匡安仁, 610041, 四川省成都市外南国学巷37号, 四川大学华西医院核医学科. kuanganren＠263.net
电话: 028-85422696 传真: 028-85422696
收稿日期: 2007-08-16 修回日期: 2007-11-02
Construction of the alpha-fetoprotein enhancer controlled gene therapy vector specific for hepatocellular carcinoma and identification of its activity
Xiao-Juan Ma, An-Ren Kuang, Rui Huang
Xiao-Juan Ma, An-Ren Kuang, Rui Huang, Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Supported by: National Natural Science Foundation of China, No. 30670585
Correspondence to: Dr. An-Ren Kuang, Department of Nuclear Medicine, West China Hospital, Sichuan University, 37 Wainanguoxue Lane, Chengdu 610041, Sichuan Province, China. kuanganren@263.net
Received: 2007-08-16 Revised: 2007-11-02
Abstract
AIM: To construct a gene-modified hepatocellular carcinoma (HCC)-specific luciferase expression vector regulated by alpha fetoprotein (AFP) promoter and enhancer, and to evaluate the effects.
METHODS: The minimal essential DNA fragment of the AFP gene promoter and enhancer was amplified through PCR from the genome of HepG2 cells. It was then cloned into the plasmid pGL-3 after the AFP promoter was combined with a 0.4- or 1.0-kb enhancer region, to construct the recombinant plasmids APSE-Luc and APLE-Luc. These two plasmids were transfected into HepG2, SMMC7721 and Hela cells. Fluorescence was detected in order to evaluate the luciferase expression and specificity.
RESULTS: By restriction digestion and sequence analysis, we confirmed that the length, position, and orientation of inserted genes of the AFP promoter and enhancer were all correct. The recombinant vector APSE-Luc/APLE-Luc was successfully constructed ......
您现在查看是摘要介绍页，详见PDF附件(589KB，5页)。