基因芯片技术检测西藏拉萨地区的乙型肝炎病毒基因型
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曹占良, 高英堂, 刘 霜, 景 丽, 吉 宗, 刘 彤, 刘持佳, 杜 智
乙型肝炎病毒;基因型;基因芯片;序列分析,曹占良,刘持佳,高英堂,景丽,刘彤,杜智,刘霜,吉宗,曹占良,通讯作者:,DetectionofHBVgenotypesinLhasabygenechip,Zhan-LiangCao,Chi-Jia
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曹占良, 刘持佳, 武警医学院附属医院检验科 天津市 300162
高英堂, 景丽, 刘彤, 杜智, 天津市第三中心医院 天津市人工细胞重点实验室 天津市 300170
刘霜, 天津市人民医院检验科 天津市 300120
吉宗, 武警西藏总队医院检验科 西藏自治区拉萨市 850000
曹占良, 2002年第三军医大学本科毕业, 学士学位, 副主任技师. 主要从事免疫、生化检验工作.
天津市科委攻关项目, No. 05YFSZSF02500
武警医学院院级科研基金项目, No. WY2004-15
通讯作者: 高英堂, 300170, 天津市河东区津塘路83号, 天津市第三中心医院. gaoyt816@163.com
电话: 022-84112148
收稿日期: 2007-08-09 修回日期: 2007-10-30
Detection of HBV genotypes in Lhasa by gene chip
Zhan-Liang Cao, Ying-Tang Gao, Shuang Liu, Li Jing, Zong Ji, Tong Liu, Chi-Jia Liu, Zhi Du
Zhan-Liang Cao, Chi-Jia Liu, Department of Laboratory, Affiliated Hospital of Medical College of Chinese People′s Armed Police Force, Tianjin 300162, China
Ying-Tang Gao, Li Jing, Tong Liu, Zhi Du, Artificial Cells Key Lab of Tianjin, Tianjin Third Central Hospital, Tianjin 300170, China
Shuang Liu, Department of Laboratory, Tianjin People's Hospital, Tianjin 300120, China
Zong Ji, Department of Laboratory, Tibet Corps Hospital of Chinese Armed Police Forces, Lhasa 850000, Tibet Autonomous Region, China
Supported by: the Key Research Project of the Science Committee of Tianjin, No. 05YFSZSF02500; Science Foundation of Medical College of Chinese People′s Armed Police Force, No. WY2004-15
Correspondence to: Dr. Ying-Tang Gao, Artificial Cells Key Lab of Tianjin, Tianjin Third Central Hospital, 83 Jintang Road, Hedong District, Tianjin 300170, China. gaoyt816@163.com
Received: 2007-08-09 Revised: 2007-10-30
Abstract
AIM: To investigate the distribution and characteristics of HBV genotypes in Lhasa.
METHODS: A total of 92 serum samples were collected from chronic hepatitis patients in Lhasa infected with HBV. Oligonucleotide probes were designed according to the DNA sequences from GenBank and used to prepare the genotyping chip. The fragments of HBV S gene were amplified using nested PCR ......
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