Ghrelin对糖尿病小鼠胃排空的影响及机制
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邱文才, 王维刚, 王志刚, 郑 起
胃排空;Ghrelin;Nω-硝基-L-精氨酸甲酯;生长激素促分泌素受体;糖尿病小鼠,邱文才,王维刚,王志刚,郑起,通讯作者:,Effectofghrelinongastricemptyingindiabeticmiceanditsmechasnism
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邱文才, 王维刚, 王志刚, 郑起, 上海交通大学医学院附属第六人民医院普外科 上海市 200233
国家自然科学基金资助项目, No. 30400429
通讯作者: 郑起, 200233, 上海交通大学医学院附属第六人民医院普外科. sh6_zhengqi@126.com
电话: 021-64369181
收稿日期: 2007-05-25 修回日期: 2007-10-03
Effect of ghrelin on gastric emptying in diabetic mice and its mechasnism
Wen-Cai Qiu, Wei-Gang Wang, Zhi-Gang Wang, Qi Zheng
Wen-Cai Qiu, Wei-Gang Wang, Zhi-Gang Wang, Qi Zheng, Department of General Surgery, the Sixth Affiliated Hospital of Medical School, Shanghai Jiaotong University, Shanghai 200233, China
Supported by: National Nature Science Foundation of China, No. 30400429
Correspondence to: Qi Zheng, Department of General Surgery, the Sixth Affiliated Hospital of Medical School, Shanghai Jiaotong University, Shanghai 200233, China. sh6_zhengqi@126.com
Received: 2007-05-25 Revised: 2007-10-03
Abstract
AIM: To investigate the gastroprokinetic effect of ghrelin in diabetic mice and its mechanism.
METHODS: The effects of ghrelin (50, 100 and 200 μg/kg) on gastric emptying in diabetic mice were after intragastric application of phenol red in vivo. The effects of ghrelin on the spontaneous contraction of fundic stomach muscle strips in the presence of ghrelin on the spontaneous contraction of fundic muscle strips from the stomachs of diabetic mice were studied in vitro. The effects of atropine and NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) were also investigated.
RESULTS: Ghrelin accelerated gastric emptying in diabetic mice with a significant dose-response relationship. Administration of ghrelin to tissue increased the spontaneous contraction of fundic stomach muscle strips from diabetic mice (1.11 ± 0.03, 1.22 ± 0.02, 1.36 ± 0.04 vs 1.00, all P < 0.05). In the presence of atropine or L-NAME, ghrelin failed to increase the spontaneous contraction of these strips.
CONCLUSION: Ghrelin can accelerate gastric emptying in diabetic mice, perhaps by activating the GHS-R cholinergic excitatory pathways and nitrergic nervous pathways in the enteric nervous system ......
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