体外循环状态下新生小型猪肝细胞的活性
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张世昌, 王英杰, 陈 志, 刘 涛, 刘 俊
肝细胞;体外循环;小型猪,张世昌,王英杰,陈志,刘涛,刘俊,张世昌,通讯作者:, ,Preliminarystudyoftheviabilityofneonatalmini-porcinehepatocytesinextracorporealc
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张世昌, 王英杰, 陈志, 刘涛, 刘俊,中国人民解放军第三军医大学西南医院感染病研究所 重庆市 400038
张世昌, 博士, 主管技师, 主要从事生物人工肝和肝组织工程的相关基础研究.
国家自然基金资助项目, No. 30470458
通讯作者: 王英杰, 400038, 重庆市沙坪坝区高滩岩正街29号, 中国人民解放军第三军医大学西南医院感染病研究所. yingjiewng@yahoo.com
电话: 023-68754475-8062 传真: 023-68754475
收稿日期: 2007-06-19 修回日期: 2007-11-28
Preliminary study of the viability of neonatal mini-porcine hepatocytes in extracorporeal circulation
Shi-Chang Zhang, Ying-Jie Wang, Zhi Chen, Tao Liu, Jun Liu
Shi-Chang Zhang, Ying-Jie Wang, Zhi Chen, Tao Liu, Jun Liu, Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China
Supported by: National Natural Science Foundation of China, No. 30470458
Correspondence to: Ying-Jie Wang, Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University of Chinese PLA, 29 Gaotanyan Center Street, Shapingba District, Chongqing 400038, China. yingjiewng@yahoo.com
Received: 2007-06-19 Revised: 2007-11-28
Abstract
AIM: To observe the viability of neonatal mini-porcine hepatocytes in extracorporeal circulation.
METHODS: A hepatocyte circulation system was constructed with Cello artificial capillary culture equipment and freshly isolated hepatocyte suspensions were circulated at 50 mL/min in vitro. The viability, function and morphological characteristics of hepatocytes were examined within 8 hours.
RESULTS: After 4 hours circulation, the viability and adherence ratio of hepatocytes were 76.1% ± 1.4% and 62.8% ± 1.8%, respectively, and the ratio of amino clearance was about 62.7% ± 14.6% of that in the control group. However, with time, many fragments of hepatocytes were observed in the circulating suspensions, as well as a significant increase in lactate dehydrogenase and aspartate aminotransferase (P < 0.01). The cell viability and adherence ratio, synthesis of urea and albumin, and the clearance rate of ammonia also decreased significantly (P < 0 ......
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