Hplc法快速测定5-氟尿嘧啶血药浓度
刘传华 刘师莲 卞继峰 耿昭 卢翌 胡海燕
摘 要：目的：建立反相高效液相色谱法测定人血清中5-氟尿嘧啶浓度。方法：血清样品用乙酸乙酯提取，水浴氮气吹干，残留物用水溶解后进样。色谱柱为C18柱(250mm×4.6mm)，水为流动相，流速1.0mL·min-1，紫外检测波长273nm，桂皮酸为内标。结果：本法最低检测浓度为0.05μg·mL-1，线性范围为1.0～50.0μg·mL-1，日内RSD为2.7%～4.1%，日间RSD为3.8%～4.7(n=4)。结论：该法适用于5-氟尿嘧啶的药代动力学研究及临床血药浓度检测。试验结果表明，该方法经济、简便、快速、灵敏度高、重现性好。
关键词：高效液相色谱 5-氟尿嘧啶 血药浓度
分类号：R927 文献标识码：A
A Simple and Rapid HPLC Determination of 5-Fluorouracil in Human Serum
, 百拇医药
LIU Chuan-hua(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
LIU Shi-lian(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
BIAN Ji-feng(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
GENG Zhao(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
, http://www.100md.com
LU Yi(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
HU Hai-yan(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
Abstract：Objective：To develop a simple and rapid HPLC method for the determination of 5-fluorouracil (5-Fu)concentration in human serum.Methods:The serum samples were extracted with ethyl acetate.The solvent was evaporated to dryness under a gentle stream of N2 at 40 ℃ in a water bath.The analysis involved a C18 column as stationary phase and distilled water as mobile phase.The flow rate was 1.0 mL·min-1,UV detection wavelength was at 273 nm.Cinnamic acid was used as the internal standard.Results:The minimal detectable serum drug concentration was 0.05 μg·mL-1.The calibration curve was linear over a range from 1.0 μg·mL-1 to 50.0 μg·mL-1 with a correlation coefficient of 0.999 4.Intra-day and inter-day RSD were 2.7%～4.1% and 3.8%～4.7%(n=4) respectively.Conclusion:The method is economic ,simple,rapid,sensitive and accurate.
, http://www.100md.com
Keywords：HPLC,5-fluorouracil,serum drug concentration
作者单位：刘传华(山东大学医学院分子生物学实验室济南 250012)
刘师莲(山东大学医学院分子生物学实验室济南 250012)
卞继峰(山东大学医学院分子生物学实验室济南 250012)
耿昭(山东大学医学院分子生物学实验室济南 250012)
卢翌(山东大学医学院分子生物学实验室济南 250012)
胡海燕(山东大学医学院分子生物学实验室济南 250012)
参考文献：
, 百拇医药
[1]Speyer J L.A study on 5-Fluorouracil in advanced malignant tumors.Semin Oncol,1985,12(3):23
[2]QING San-hua(卿三华),ZHOU Xi-geng(周锡庚),XU Jun-chang(许俊昌),et al.Studies on 5-fluorouracil in human serum,biological fluid and tissue by HPLC(高效液相色谱法测定血液、体腔和组织中5-Fu浓度的研究).Tumor(肿瘤),1990,10(4)：168(in Chinese)
[3]WANG Dong-mei(王冬梅),XIE Jing-wen(谢景文).Determination of 5-Fluorouracil concentration in human serum by HPLC(高效液相色谱法测定人血清中5-氟尿嘧啶浓度).Chin J Chromatogr(色谱),1997,15(2)：164(in Chinese), http://www.100md.com
摘 要：目的：建立反相高效液相色谱法测定人血清中5-氟尿嘧啶浓度。方法：血清样品用乙酸乙酯提取，水浴氮气吹干，残留物用水溶解后进样。色谱柱为C18柱(250mm×4.6mm)，水为流动相，流速1.0mL·min-1，紫外检测波长273nm，桂皮酸为内标。结果：本法最低检测浓度为0.05μg·mL-1，线性范围为1.0～50.0μg·mL-1，日内RSD为2.7%～4.1%，日间RSD为3.8%～4.7(n=4)。结论：该法适用于5-氟尿嘧啶的药代动力学研究及临床血药浓度检测。试验结果表明，该方法经济、简便、快速、灵敏度高、重现性好。
关键词：高效液相色谱 5-氟尿嘧啶 血药浓度
分类号：R927 文献标识码：A
A Simple and Rapid HPLC Determination of 5-Fluorouracil in Human Serum
, 百拇医药
LIU Chuan-hua(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
LIU Shi-lian(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
BIAN Ji-feng(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
GENG Zhao(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
, http://www.100md.com
LU Yi(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
HU Hai-yan(Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012)
Abstract：Objective：To develop a simple and rapid HPLC method for the determination of 5-fluorouracil (5-Fu)concentration in human serum.Methods:The serum samples were extracted with ethyl acetate.The solvent was evaporated to dryness under a gentle stream of N2 at 40 ℃ in a water bath.The analysis involved a C18 column as stationary phase and distilled water as mobile phase.The flow rate was 1.0 mL·min-1,UV detection wavelength was at 273 nm.Cinnamic acid was used as the internal standard.Results:The minimal detectable serum drug concentration was 0.05 μg·mL-1.The calibration curve was linear over a range from 1.0 μg·mL-1 to 50.0 μg·mL-1 with a correlation coefficient of 0.999 4.Intra-day and inter-day RSD were 2.7%～4.1% and 3.8%～4.7%(n=4) respectively.Conclusion:The method is economic ,simple,rapid,sensitive and accurate.
, http://www.100md.com
Keywords：HPLC,5-fluorouracil,serum drug concentration
作者单位：刘传华(山东大学医学院分子生物学实验室济南 250012)
刘师莲(山东大学医学院分子生物学实验室济南 250012)
卞继峰(山东大学医学院分子生物学实验室济南 250012)
耿昭(山东大学医学院分子生物学实验室济南 250012)
卢翌(山东大学医学院分子生物学实验室济南 250012)
胡海燕(山东大学医学院分子生物学实验室济南 250012)
参考文献：
, 百拇医药
[1]Speyer J L.A study on 5-Fluorouracil in advanced malignant tumors.Semin Oncol,1985,12(3):23
[2]QING San-hua(卿三华),ZHOU Xi-geng(周锡庚),XU Jun-chang(许俊昌),et al.Studies on 5-fluorouracil in human serum,biological fluid and tissue by HPLC(高效液相色谱法测定血液、体腔和组织中5-Fu浓度的研究).Tumor(肿瘤),1990,10(4)：168(in Chinese)
[3]WANG Dong-mei(王冬梅),XIE Jing-wen(谢景文).Determination of 5-Fluorouracil concentration in human serum by HPLC(高效液相色谱法测定人血清中5-氟尿嘧啶浓度).Chin J Chromatogr(色谱),1997,15(2)：164(in Chinese), http://www.100md.com