Volume10 May2003 Number3
CHINESE JOURNAL OF INTEGRATED TRADITIONAL
AND WESTERN MEDICINE IN INTENSIVE AND CRITICAL CARE
Volume 10 May 2003 Number 3
PAGE：131-134
Inhibitory effect of egg white lysozyme on ceftazidime-induced release of lipopolysaccharide from Shigella dysenteriae
WANG Dan, LIANG Ai-hua
Institute of Chinese Materia Medical, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
, 百拇医药
Abstract： Objective： To investigate the inhibition of lysozyme (LZM) on ceftazidime (CFT) induced lipopolysaccharide (LPS) release through experiments both in vitro and in vivo. Methods： Shigella dysenteriae ATCC 51067 which was cultured in the nutrition broth or the diluted rabbit blood was treated with CFT, a β-lactam antibiotic, alone or in combination with LZM. The culture supernatants were collected by centrifuge at 3 hours after addition of CFT. The concentrations of LPS in the supernatants were tested. The diluted rabbit blood culture supernatants were added into RAW 264.7 cells, a macrophage cell line. Tumor necrosis factor-α(TNF-α) productivity of the supernatants from the cell cultures was assayed with dye ingestion methods. Specific pathogen-free male institute for cancer research(ICR) mice were challenged with intravenous (i.v.) injection of the nutrition broth culture supernatants. TNF-α induced in mice was assayed with dye ingestion methods. Rabbits were challenged with intraperitoned injection of Shigella dysenteriae nutrition broth culture suspension, and then CFT or CFT and LZM were intraperitoned injected for various groups of rabbits 3 hours later. LPS, TNF-α and nitric oxide(NO) were investigated in the rabbit plasm. Results： CFT (50 mg/L, 100 mg/L) caused high concentrations of LPS in the culture supernatants. The nutrition broth culture supernatants induced high level of TNF-α production in mouse plasm. The diluted rabbit blood culture supernatants induced high level of TNF-α production in RAW 264.7 cells. LZM in combination with CFT resulted in a decreased LPS concentration in the culture supernatants as well as a low TNF-α production both in mouse plasm and in RAW 264.7 cells. The combination of LZM with CFT could abate LPS concentration and reduce release of inflammatory substances assessed by TNF-α and NO in rabbit plasm. Conclusion： LZM may inhibit CFT-induced LPS release from Shigella dysenteriae.
, 百拇医药
PAGE：135-137
Proliferation of hematopoietic progenitor cells from human bone marrow induced by panax notoginosides(三七总皂苷)
ZHENG Yin-hong, GAO Rui-lan, ZHU Da-yuan, QIAN Xu-dai, NIU Yang-ping
Affiliated Hospital, Zhejiang Traditional Chinese Medical College, Hangzhou 310006, Zhejiang, China.
Abstract： Objective： To investigate the proliferation efficiency of both panax notoginosides (PNS, 三七总皂苷) and monomers on hematopoietic progenitor cells of colony-forming unit-granulocyte macrophage (CFU-GM) and colony-forming unit-erythroid (CFU-E). Methods： Human bone marrow cells were incubated with semi -solid culture systems in the presence of vary concentration of PNS and its monomers of Rg1, Rb1, Re+R1 for seven days. The increasing rate of CFU-GM and CFU-E colony formation induced by PNS was estimated in vitro. Results： ①The hematopoietic progenitor cells from bone marrow were proliferated in the response to stimulation of PNS. After being incubated with PNS 8, 40 and 100 mg/L, the increasing rates of CFU-GM colony forming were (32.5±6.8)%, (59.3±8.7)% and (37.0±1.6)% respectively (all P<0.01). The CFU-E colonies was raised by (33.3±7.3)% (P<0.01) at the concentration of PNS 40 mg/L. ②The monomer Rg1 could raise the colony formation of CFU-GM with increasing rate of (33.5±3.8)% and (59.7±4.7)% (both P<0.01) when the cells were exposed to Rg1 8 and 40 mg/L respectively. While 40 mg/L of Rb1 could elevate CFU-E colony by (47.3±6.3)% (P<0.01). However, the monomer Re+R1 did not raise the colony formation of both CFU-GM and CFU-E. Conclusion： PNS can promote the proliferation on hematopoietic progenitor cells of CFU-GM and CFU-E. Meanwhile, Rg1 and Rb1 isolated from PNS are the effective monomer to enhance the proliferation on CFU–GM and CFU-E respectively.
, 百拇医药
PAGE：138-141
Effect of sodium ferulate on activation of postsynaptic density-95 after transient focal cerebral ischemia
WANG Qiang, XIONG Li-ze, CHEN Shao-yang, JIN Wei-lin, HU Sheng, DONG Hu
Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shanxi, China.
Abstract： Objective： To study the neuroprotection of sodium ferulate (SF) and effect of SF on activation of postsynaptic density-95 (PSD-95) after transient cerebral artery occlusion in rats. Methods： Forty-six male Sprague-Dawley rats were randomly divided into two groups (n=23 in each group)： control group and SF group. They received intraperitoneal normal saline 4 ml and SF 100 mg/kg which was dissolved in 4 ml normal saline respectively when the rats were ischemic. Middle cerebral artery occlusion (MCAO) model was used. At 24 hours after reperfusion, 16 animals (n=8 in each group) were assessed by neurological scale, and brain tissue was taken to assess the infarct volume with 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The other rats(n=3 at different timepoint in each group) were decapitated at 30 minutes, 2 hours, 6 hours, 24 hours and 72 hours after reperfusion. Immediately the activation of PSD-95 was measured using Western blot analysis system in ischemia and contralateral cortex and striatum of rat brain. Results： All the ratssurvived after operation. The neurologic deficit score of rats in SF group decreased significantly compared with control group(P<0.01). The infarct volume of control group 〔(168.1±42.2)mm3〕 was significant greater than those of SF group〔(61.5±28.7) mm3,P<0.01〕 at 24 hours after reperfusion. The ischemic cortex and striatum in control group showed a markedly increased level of PSD-95 activation and peaked at 30 minutes after reperfusion. Activation of PSD-95 was moderately increased 30 minutes after reperfusion, and peaked at 2 hours, followed by a moderate decline at 6 hours in SF group. Moreover, activation of PSD-95 at each time point in SF group was less than that in control group, and activation of PSD-95 persisted a shorter course in SF group than in control group. No upregulation of PSD-95 protein could be detected in the contralateral cortex. Conclusion： SF could induce neuroprotective effects in transient focal cerebral ischemic injury, weaken PSD-95 activation in ischemic area.
, 百拇医药
PAGE：142-145
Effects and mechanisms of panaxadiol saponins(人参二醇组皂甙) promoting the proliferation of scathing cultured human renal tubular cells
PAN Qiang-wen, RAN Bing, FENG Zhi-qiang, GUO Yong, WANG Qiong
Laboratory of Renal Function of Luzhou Medical College, Luzhou 646000, Sichuan,China.
Abstract： Objective ： To study the effects and mechanisms of panaxadiol saponins(PDS, 人参二醇组皂甙) promoting the proliferation of normally cultured human renal tubular cells and the cells which suffered by the rabbit serum of acute renal ischemic-reperfusion. Methods： The bilateral renal arteries of rabbits were occluded then reperfusion and the serum (SIR) were acquired. The rabbit serums of control (SC) were acquired in the same time. The proliferative activity of cells was measured with the method of MTT. And the rates of living cells were counted. Results： The value of optical density(OD, as A) in 10 mg/L PDS group (0.62766±0.0392) was significant higher than that in control groups (0.5075±0.0597)， P<0.05. The value of A and the rate of living cells in SIR group (0.1989±0.0313 and 46.0%) were significant lower than those in other groups (0.6594-0.7850 and 91.5%-98.9%). But there were not significant differences in the proliferative activity of PDS +SIR groups (0.6594±0.1678) and control group (0.7350±0.0812). Conclusion： Moderate panaxadiol saponins could accelerate the proliferation of normally cultured human renal tubular cells and the cells which suffered by the rabbit serum of acute renal ischemic-reperfusion.
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PAGE：146-148
Effects of ginkgo biloba extract(银杏叶提取物) on platelet activating factor in rats with nephrotoxic serum glomerulonephritis
LIAN Xi-yan, YANG Ling, CHEN Qing, SHEN An-bei, ZHAO Jin-tao
Departmentof Nephrology, the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan, China.
Abstract：Objective： To investigate the effect of ginkgo biloba extract(银杏叶提取物) on platelet activating factor(PAF) and the protection on renal function in rat with nephrotoxic serum glomerulonephritis (as Masugi glomerulonephritis). Methods： The rat model of Masugi glomerulonephritis was induced by Vasslli's methods. Twenty-four animals were randomly divided into the model group, treatment group and non-treated control group. The rats were treated with ginkgo biloba extract. PAF and proteinuria in urine were measured before and after treatment. PAF in serum, renal cortex and renal medulla as well as serum creatinine were also measured after treatment. The effect of ginkgo biloba extract on the renal histopathology in this model was observed. Results：Compared to the model group, ginkgo biloba extract could significantly decrease proteinuria and PAF in urine of 24 hours (P<0.05), serum(P0.05), the renal cortex (P<0.05) and renal medulla, especially in the renal cortex.Prominent histopathological changes and improvement of renal function (P<0.01) were observed in treatment group. Conclusion： Ginkgo biloba extract can inhibit PAF level and has beneficial effects on Masugi glomerulonephritis.
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PAGE：149-151
Study of Xuezhongxiao(血肿消) on pre-protection of kidney in hypertensive cerebral hemorrhage
WANG Yu-qin, WANG Zhen-yu, WANG Ai-feng, WANG Song-ling
Henan Traditional Chinese Medical Hospital, Zhengzhou 450002, Henan, China.
Abstract：Objective： To study the pre-protective effects of integrated traditional Chinese and western medicine on kidney in hypertensive cerebral hemorrhage. Methods： Ninety hypertensive cerebral hemorrhagic patients were randomly divided into three groups： Xuezhongxiao (血肿消) combined with dopamine treatment group(n=30), dopamine treatment group(n=30) and medical treatment group(n=30). The same manitol treatment (250 ml, 1 time per 6 hours) and conventional therapy were used in the three groups. The clinical effects, renal function before and after treatment, absorption of cerebral haematoma and the incidence rate of renal injury were observed in the three groups. Results： The clinical effects and incidence rate of renal injury were significantly different among the three groups (all P<0.05). After treatment, the curative effects in Xuezhongxiao combined with dopamine treatment group was superior to those in dopamine treatment group 〔the total curative effect： 96.67% vs. 90.00%; blood urea nitrogen: (5.72±0.58) mmol/L vs. (6.90±5.35) mmol/L; serum creatinine： (91.00±20.11) μmol/L vs. (98.31±37.66) μmol/L; incidence rate of kidney injury： 3.33% vs. 6.67%; mortality： 0 vs. 0〕, and those in dopamine treatment group were superior to those in medical treatment group 〔63.33%, (7.80±3.67)mmol/L, (148.62±42.02)μmol/L, 26.66% and 6.67% respectively〕. The absorption of haematoma was (95.60±16.21)%, (87.10±12.70)% and (76.35±14.36)% in three groups respectively(all P<0.05). Conclusion： Xuezhongxiao can prevent renal injury induced by manitol, it can quicken absorption of haematoma, raise curative rate and reduce mortality. The curative effect of Xuezhongxiao combined with dopamine is the best in three groups.
, 百拇医药
PAGE：152-154
Clinical research of treatment with high-dose intravenous γ-globulin combined low dose corticosteriod in severe idiopathic thrombocytopenic purpura
FANG Bing-mu, LIU Ying, LI Lin-jie, LIU Wei-hong, JIN Yang-jin
Department of Hematology, Zhejiang Lishui City Central Hospital, Fifth Affiliated Hospital of Wenzhou Medical College, Lishui 323000, Zhejiang, China.
, 百拇医药
Abstract： Objective ： To evaluate the effectiveness of high-dose intravenous γ-globulin (HDIG) combine with low dose corticosteroid in severe idiopathic thrombocytopenic purpura (ITP). Methods： A 6-year retrospective study of 75 patients hospitalized with severe ITP was conducted to compare the effectiveness of corticosteroid and HDIG. HDIG group was given γ-globulin 0.4 g·kg-1·d-1 for 5 days, and prednisolone 0.5 mg· kg-1·d-1 for 28 days. Corticosteroid group was given prednisolone 1-2 mg·kg-1·d-1 for 28 days. Results： In HDIG group, 25 (65.8%) cases were markedly improved, 9 cases (23.7%) were more improved, 2 cases (5.3%) were improved, 2 cases (5.3%) showed no effect, the total effective rate was 94.7%, and they were 23 cases (62.2%), 9 cases (24.3%), 3 cases (8.1%), 2 cases (5.4%) and 94.6% in corticosteroid group. Blood platelet count(BPC) increased significantly after treatment in both two groups compared to those before treatment (both P<0.01), and BPC-peak values in the HDIG group〔(212.56±90.25)×109/L〕 were higher than that in the corticosteroid group 〔(127.26±81.26)×109/L〕. After treatment the time of getting BPC-peak value in the HDIG group〔(7.80±4.50) d〕 was shorter than that in corticosteroid group〔(27.00±9.32)d〕. Conclusion： HDIG combined low dose corticosteroid is more effectively in increasing the platelet count rapidly to safe levels in severe ITP.
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PAGE：155-157
Effect of Xiaoke Antang prescription(消渴安糖方) on the levels of gastrointestinal hormones in patients with type 2 diabetes mellitus of Qi-Yin deficiency syndrome(气阴两虚证)
LIN Shou-ning, LIU Peng, ZHOU Zhuo-ning, WEI Xiao-hong, TAN Chi.
The Second Affiliated Hospital, Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi, China.
Abstract ： Objective： To study the effect of Xiaoke Antang prescription (消渴安糖方) on the serum motilin (MTL), pancreatic glucagons (PG) and substance P (SP) in patients with type 2 diabetes mellitus (DM) of Qi-Yin deficiency syndrome (气阴两虚证), and to observe its therapeutic effects on patients with Qi-Yin deficiency syndrome in diabetic neuropathy. Methods： Ninety cases with type 2 DM of Qi-Yin deficiency syndrome were randomly divided into observation group and control group, 30 healthy people were selected as control. In addition to regular therapy of DM, the observation group was additionally treated with XiaokeAntang prescription. The two groups were observed for a month. MTL, PG and SP were measured by radioimmunoassay before and after treatment, fasting blood sugar (FBS) and 2 hours postprandial blood sugar (PBS) were also measured. During the treatment the syndrome score of gastrointestinal function disorder were observed. MTL, PG and SP of the healthy control group were tested only one time. Results： The levels of MTL and PG in type 2 DM patients of the two groups were higher than the healthy normal group, but the level of SP was lower. There were significant differences between the levels of three above hormones of the two observation groups and that of the normal control group(all P<0.01), and there were great improvement in the abnormal of MTL, PG, SP and in the syndromes of gastrointestinal function disorder after treatment compared with those before treatment (all P<0.01). The improvement of blood sugar(BS) of the observation group was superior to that of the control group(all P<0.05). Conclusion： Xiaoke Antang prescription can betterly improve the abnormal of MTL, PG and SP of Qi-Yin deficiency syndrome in patients with type 2 DM, it proves that Qi-Yin deficiency syndrome in type 2 DM has relativity with the abnormal of MTL, PG and SP. Xiaoke Antang prescription can provide better therapeutic effects on Qi-Yin deficiency syndrome in patients with diabetic neuropathy, and has certainly auxiliary effect on reducing the level of BS.
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PAGE：158-160
Relation between pathogenesis of traditional Chinese medicine and hepatitis B virus-DNA or T lymphocytes in chronic hepatitis B
YANG Hong-zhi, BIAN Zhuang, WANG Yong-ze, GUAN Wei-bing, YANG Yue-wu
The Third Hospital Affiliated Sun Yat-sen University, Guangzhou 510630, Guangdong, China.
Abstract： Objective ：To provide objective indexes for clinical syndrome differentiation in patients with chronic hepatitis B, and research the relation between pathogenesis and hepatitis B virus (HBV-DNA) or T lymphocytes. Methods： The eligible patients with slight and moderate chronic hepatitis B, HBeAg-positive and detectable copy of HBV-DNA in serum were divided into sthenia syndrome (实证, SS) group, intermingled asthenia and sthenia syndrome (虚实兼夹证, IASS) group and asthenia syndrome (虚证, AS) group. The indexes were analyzed in research groups. Results： Compared to controls, level of CD 4 cell in patients with chronic hepatitis B decreased, while there was a significant difference in level of CD+4/CD+8 ratio. The levels of HBV-DNA were SS>IASS>AS(P<0.05). The level of CD+4/CD+8 ratio was AS>IASS>SS(P<0.05). Conclusion： It is suggested that there is disorder in immune regulation in hepatitis B patients. The levels of HBV-DNA could be regarded as the index of SS. The proportion of CD+8 could be regarded as the index of AS. The value of CD+4/CD+8 could be regarded as the index of IASS.
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PAGE：161-163
Effects of Xiaoxintong capsule(消心痛胶囊) on electrocardiogram segment ST and wave T of rats with acute myocardial ischemia
TENG Jiu-xiang, CHEN Xiao-yang, JIANG Meng-liang, PENG Zhi-pei, HAN Guo-dong, SUN Gui-Xiang
Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract： Objective： To investigate effects of Xiaoxintong capsule (消心痛胶囊) on electrocardiogram(ECG) segment ST, elevation of wave T, time and incidence of segment ST and wave T in excess of 0.2 mV. Methods： Rat's acute myocardial infarction (AMI) model was built by using the Pituitrin of hypophysis cerebri. Segment ST and wave T of ECGⅡ were observed as the indexes of the AMI improvement in the medical group. The effects of Xiaoxintong capsule and Diao Xinxuekang capsule(地奥心血康胶囊) were observed. Results： Compared to model group, Xiaoxintong capsule could greatly improve the elevation of segment ST and wave T of the ischemia(P<0.05), reduce the incidence and the time for elevation(both P<0.05). The effect was almost the same between high dosage of Xiaoxintong capsule group and Diao Xinxuekang capsule group. At different time slot, compared to the model group, high dosage of Xiaoxintong capsule group had an obvious significance within one minute (all P<0.01). It could significantly descend the elevation of segment ST and wave T caused by Pituitrin of hypophysis cerebri. Conclusion： Xiaoxintong capsule has good preventive and therapeutic effects of anti-myocardial ischemia. It maybe associate with the function of expanding coronary artery, enhancing blood circulation, improving the blood supply of the heart muscle and its endurance of the oxygen deficiency and regulating the balance of the whole organism.
, 百拇医药
PAGE：164-166
Effect of Feixin mixture(肺心合剂) on monocrotaline induced pulmonary hypertension in rats
YANG Mao-nong, WANG Fei, XU Fang-yuan
The People's Hospital of Sichuan Province, Chengdu 610072, Sichuan, China.
Abstract： Objective： To explore the effect of Feixin mixture(FXM, 肺心合剂) on pulmonary hypertension (PAHT). Methods： Adult male Wistar rats were given a single dose of subcutaneous monocrotaline(MCT, 50 mg/kg) to induce the model of pulmonary hypertension. Sixty cases were randomly divided into normal contral group, PAHT mold group, FXM group and nifedipine group (n=10). Then the rats were treated with gastric infusion of normal saline, FXM or nifedipine in 14th days respectively. The parameters of pulmonary hemodynamics were monitored by Gould3400 polygraph system through the cannulation of polyvinyl tube. Right ventricle and left ventricle added with interventricular septum weight ratio, the lung wet weight and body weight ratio were measured. Results： FXM and nifedipine significantly inhibited the progression of pulmonary artery pressure and decreased the right heart index and reduced the seepage out of the lung (P<0.05 or P<0.01). The effect of high dose FXM group was better. Conclusion： FXM is able to reduce the pulmonary hypertension and improved the heart function.
, 百拇医药
PAGE：167-169
Effect of Xinlening concentrates(心乐宁浓缩液) on hemodynamics in dog with heart failure
ZHI Li-qin, PEI Ji-wen, LI Xi-kuan
Department of Cardiology, Traditional Chinese Medicine Hospital, Xi'an 710001, Shanxi, China.
Abstract： Objective： To investigate the influence of Xinlening concentrates (心乐宁浓缩液) on hemo dynamics in dogs with failure heart. Methods： The experimental dogs were randomly divided into six groups. Negative control group was infused with normal saline, heart failure group was infused with normal saline, and different doses of Xinlening concentrates groups were given Xinlening concentrates 16 g/kg, 32 g/kg, and 64 g/kg respectively. Positive control group was treated with digoxin. The cardiac output was measured with the magnetic volumetric meter at the root of aorta. The inner pressure of left ventricle and the systemic artery pressure were measured by insertion of catheter into carotid and femoral artery respectively. Other hemodynamic indexes were also calculated by confirmed formula. Results： A different dose of Xinlening concentrates and digoxin could mildly increase the average artery pressures. The canine cardiac output could be improved dramatically by different dosage of Xinlening concentrate, the effect of increasing cardiac output of digoxin was not significant different(P>0.05). The inner pressure of left ventricle and the working indexes were also increased with the treatment of different doses of Xinlening concentrates, similar to that of digoxin (P<0.05). The effect of large dosage of Xinlening concentrate was similar to digoxin. Conclusion： The Xinlening concentrates may improve the cardiac output and left ventricular working indexes, improve the hemodynamic status of the canines with failure heart.
, 百拇医药
PAGE：170-173
Experimental research on the effect of E-bing Shuguan drop-pill(鹅冰舒冠滴丸) on arterial lipid plague
CHENG Chou-fu, TAN Yuan-sheng, TAN Sheng-e, JIN Chao-hui. The First Affiliated Hospital, Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract： Objective： To investigate the mechanism of E-bing Shuguan drop-pill (鹅冰舒冠滴丸) on arterial lipid plague in treating coronary angina pectoris. Methods： The hyperlipemia and atherosclerosis rabbit model was established with the phago-source, and the Pituitrin was given by intravenous injection to induce the acute myocardial ischemia. The animals were randomly divided into negative control group, model group, composite salvia miltiorrhiza group and low-, middle-, high-dose of E-bing Shuguan drop-pill group. The biochemistry indexes and pathological histology changes were observed. Results： E-bing Shuguan drop-pills could regulate the blood lipid(high-dose of it had the best effect) and the balance of endothelin (ET)/nitric oxide(NO), increasing high-density lipoprotein cholesterol(compared to the composite salvia miltiorrhiza group, P<0.05), protect the endothelial cell, improve myocardial ischemia of the experimental rabbit. High-dose group had the better effect on decreasing the increased T wave at 30 minutes compared to the composite salvia miltiorrhiza group (P<0.05). E-bing Shuguan drop-pill could decrease the size of arterial lipid plague, especially in middle-and small-dose group(compared to the composite salvia miltiorrhiza group, both P<0.05). Conclusion： E-Bing Shuguan drop-pills can protect myocardial, delay atherosclerosis lipid plaque happening and developing, even extincting atherosclerosis lipid plaque.
, 百拇医药
PAGE：174-176
Study on treatment of upper digestive tract hemorrhage by Huangmi starch(黄蜜浆) and proton pump inhibitor
LIN Ping，LIAN Ming-feng，ZHANG Qiang，ZHANG Lie-hu，YANG Jian-min
The First Hospital of Longyan, Longyan 364000, Fujian, China.
Abstract： Objective： To investigate the therapeutic value and application regularity on treatment of upper digestive tract hemorrhage with traditional Chinese and western medicine Huangmi starch(黄蜜浆) and proton pump inhibitor. Methods：One hundred and thirty patients with upper digestive tract hemorrhage were randomly divided into three groups，42 patients were treated with Huangmi starch， 42 patients were treated with proton pump inhibitor，and 46 patients were treated combined with Huangmi starch and proton pump inhibitor.The therapeutic efficacy and hemostatic time were observed and compared in three groups. Results： The fully recovery rate in patients treated by Huangmi starch and proton pump inhibitor were highter than treated by proton pump inhibitor(87.0% vs.69.0%， P<0.05)，hemostatic time in patients treated by Huangmi starch and proton pump inhibitor were better than only Huangmi starch or proton pump inhibitor groups 〔the time of tarry stood turned yellow and stood occult blood disappeared: (36.5±10.2) h,(2.5±0.9) d vs.(52.3±15.6)h,(3.9±1.2)d, and (81.4±25.5)h,(5.2±1.9)d, all P<0.01〕. Conclusion：Treatment of upper digestive tract hemorrhage combine with Huangmi starch and proton pump inhibitor can shorten hemostatic time，increase therapeutic efficacy. Huangmi starch and proton pump inhibitor have synergistic action.
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PAGE：177-180
Management of acute postoperative bleeding after pancreatoduodenectomy
JIANG Kai, ZHOU Ning-xin, HUANG Zhi-qiang, LIU Rong, HUANG Xiao-qiang, ZHANG Wen-zhi, WANG Mao-qiang
The General Hospital of People's Liberation Army, Beijing 100853, China.
Abstract: Objective: To investigate the management principle and method for the acute postoperative bleeding after pancreatoduodenectomy(PDT). Methods: The experience of six patients with hemorrhage after pancreatoduodenectomy (PDT) in our hospital from 1991 to 2001 were summarized, and some literatures were reviewed for analyzing the clinical presentation, bleeding reason, management and outcome. Results: Sudden severe super abdomen pain and in/out- drainage tube bleeding of peritoneal cavity were the main manifestation for all patients, and combining supper gastrointestinal tract bleeding for two of them. All patients had pancreatic leak. Emergency arterial angiography examination was made for all cases to confirm reason of the bleeding from gastroduodenal artery (3 cases), inferior pancreatoduodenal artery (2 cases) and posterior edge of pancreatic stump (1 case), respectively. The former (3 cases) were treated by hepatic arterial embolization without rebleeding, and the latter (3 cases) were undergone by relaparotomy hemostasis. One patient died of multiple organ failure because of repeat postoperative rebleeding after 5 days. Intestine function could restore after treatment with traditional Chinese herbs of strengthening spleen and activating Qi (健脾行气). Conclusion: Necessary preoperative preparation and emergency arterial angiography should be done rapidly for all acute hemorrhage of the patients to confirm the bleeding source. Selecting hepatic aterial embolization or operation hemostasis is depended on the bleeding position.
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PAGE：181-182
Clinical study of naloxone of severe craniocerebral trauma with both corectasis
MIAO Yu-lu, XI Yin-xue, JIANG Zheng-ming, NIE Yu-lin, YU Shao-xiong, YAO Li-zhi, ZOU Shi-cai, FU Hong-jin, HUANG Zhi-bin
Buji People's Hospital, Shenzhen 518112, Guangdong, China.
Abstract： Objective： To assess clinical effects of naloxone on severe craniocerebral trauma with both corectasis. Methods： Patients (GCS were 3-8 scores) with severe craniocerebral trauma were randomly divided into two groups： treatment group and control group. Control group was treated by oxygen uptake, compression of cranium, dehydration, using antibiotic, keeping nutritive equilibrium, tracheostomy, etc. Treatment group was treated with naloxone except for control group. Observation indexes： 24 hours electrocardiograph, blood gas analysis, degree of blood oxygen saturation; breath and heart rate after 7-day treatment, hemorrhage of digestive tract after 10- day treatment, cerebral infarction, mortality, linguistic and move function after 3- week treatment. Results： Compared to the control group, the time of recovery coma was shorter 〔(10.61±4.11) days vs.(15.78±3.95) days〕, heart rate, breath rate, imbalance of acid and basic, the incidence of digestive tract hemorrhage were improved, the incidence of cerebral infarction, linguistic, and mortality were decreased (P<0.05 or P<0.01). Conclusion： Naloxone treating severe craniocerebral trauma with both corectasis have good clinical effects, significantly reduce mortality and disability, improve the recovery of coma.
, 百拇医药
PAGE：183-185
Evaluation of randomized controlled trials in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi
XUE Xin-sheng, ZHANG Zhong-wei, ZHOU Yan
ICU, Huaxi Hospital of Sichuan University, Chengdu 610041, Sichuan, China
Abstract： Objective： To evaluate the quantity of the randomized controlled trials (RCTs) papers published in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi and offer the basic information to Chinese Cochrane center. Methods： The control trials of clinical therapeutic studies in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi from 1994 to 2001 were checked to identify strictly the RCT and controlled clinical tests (CCTs) according to the criteria of the handbook of Cochrane collaboration in 1997. Results： One thousand six hundred and six papers on clinical trials were published in 67 issues covered 8 volumes of Zhongguo Zhongxiyi Jiehe Jijiu Zazhi. Among them, there were 618 papers of RCT (38.48%), 203 papers of CCT (12.64%), and the papers of RCT increased more significantly, especially after 1998, and it was 60.32% in 2000.Conclusion： The quality of RCT papers published in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi has been improved year by year., 百拇医药
AND WESTERN MEDICINE IN INTENSIVE AND CRITICAL CARE
Volume 10 May 2003 Number 3
PAGE：131-134
Inhibitory effect of egg white lysozyme on ceftazidime-induced release of lipopolysaccharide from Shigella dysenteriae
WANG Dan, LIANG Ai-hua
Institute of Chinese Materia Medical, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
, 百拇医药
Abstract： Objective： To investigate the inhibition of lysozyme (LZM) on ceftazidime (CFT) induced lipopolysaccharide (LPS) release through experiments both in vitro and in vivo. Methods： Shigella dysenteriae ATCC 51067 which was cultured in the nutrition broth or the diluted rabbit blood was treated with CFT, a β-lactam antibiotic, alone or in combination with LZM. The culture supernatants were collected by centrifuge at 3 hours after addition of CFT. The concentrations of LPS in the supernatants were tested. The diluted rabbit blood culture supernatants were added into RAW 264.7 cells, a macrophage cell line. Tumor necrosis factor-α(TNF-α) productivity of the supernatants from the cell cultures was assayed with dye ingestion methods. Specific pathogen-free male institute for cancer research(ICR) mice were challenged with intravenous (i.v.) injection of the nutrition broth culture supernatants. TNF-α induced in mice was assayed with dye ingestion methods. Rabbits were challenged with intraperitoned injection of Shigella dysenteriae nutrition broth culture suspension, and then CFT or CFT and LZM were intraperitoned injected for various groups of rabbits 3 hours later. LPS, TNF-α and nitric oxide(NO) were investigated in the rabbit plasm. Results： CFT (50 mg/L, 100 mg/L) caused high concentrations of LPS in the culture supernatants. The nutrition broth culture supernatants induced high level of TNF-α production in mouse plasm. The diluted rabbit blood culture supernatants induced high level of TNF-α production in RAW 264.7 cells. LZM in combination with CFT resulted in a decreased LPS concentration in the culture supernatants as well as a low TNF-α production both in mouse plasm and in RAW 264.7 cells. The combination of LZM with CFT could abate LPS concentration and reduce release of inflammatory substances assessed by TNF-α and NO in rabbit plasm. Conclusion： LZM may inhibit CFT-induced LPS release from Shigella dysenteriae.
, 百拇医药
PAGE：135-137
Proliferation of hematopoietic progenitor cells from human bone marrow induced by panax notoginosides(三七总皂苷)
ZHENG Yin-hong, GAO Rui-lan, ZHU Da-yuan, QIAN Xu-dai, NIU Yang-ping
Affiliated Hospital, Zhejiang Traditional Chinese Medical College, Hangzhou 310006, Zhejiang, China.
Abstract： Objective： To investigate the proliferation efficiency of both panax notoginosides (PNS, 三七总皂苷) and monomers on hematopoietic progenitor cells of colony-forming unit-granulocyte macrophage (CFU-GM) and colony-forming unit-erythroid (CFU-E). Methods： Human bone marrow cells were incubated with semi -solid culture systems in the presence of vary concentration of PNS and its monomers of Rg1, Rb1, Re+R1 for seven days. The increasing rate of CFU-GM and CFU-E colony formation induced by PNS was estimated in vitro. Results： ①The hematopoietic progenitor cells from bone marrow were proliferated in the response to stimulation of PNS. After being incubated with PNS 8, 40 and 100 mg/L, the increasing rates of CFU-GM colony forming were (32.5±6.8)%, (59.3±8.7)% and (37.0±1.6)% respectively (all P<0.01). The CFU-E colonies was raised by (33.3±7.3)% (P<0.01) at the concentration of PNS 40 mg/L. ②The monomer Rg1 could raise the colony formation of CFU-GM with increasing rate of (33.5±3.8)% and (59.7±4.7)% (both P<0.01) when the cells were exposed to Rg1 8 and 40 mg/L respectively. While 40 mg/L of Rb1 could elevate CFU-E colony by (47.3±6.3)% (P<0.01). However, the monomer Re+R1 did not raise the colony formation of both CFU-GM and CFU-E. Conclusion： PNS can promote the proliferation on hematopoietic progenitor cells of CFU-GM and CFU-E. Meanwhile, Rg1 and Rb1 isolated from PNS are the effective monomer to enhance the proliferation on CFU–GM and CFU-E respectively.
, 百拇医药
PAGE：138-141
Effect of sodium ferulate on activation of postsynaptic density-95 after transient focal cerebral ischemia
WANG Qiang, XIONG Li-ze, CHEN Shao-yang, JIN Wei-lin, HU Sheng, DONG Hu
Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shanxi, China.
Abstract： Objective： To study the neuroprotection of sodium ferulate (SF) and effect of SF on activation of postsynaptic density-95 (PSD-95) after transient cerebral artery occlusion in rats. Methods： Forty-six male Sprague-Dawley rats were randomly divided into two groups (n=23 in each group)： control group and SF group. They received intraperitoneal normal saline 4 ml and SF 100 mg/kg which was dissolved in 4 ml normal saline respectively when the rats were ischemic. Middle cerebral artery occlusion (MCAO) model was used. At 24 hours after reperfusion, 16 animals (n=8 in each group) were assessed by neurological scale, and brain tissue was taken to assess the infarct volume with 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The other rats(n=3 at different timepoint in each group) were decapitated at 30 minutes, 2 hours, 6 hours, 24 hours and 72 hours after reperfusion. Immediately the activation of PSD-95 was measured using Western blot analysis system in ischemia and contralateral cortex and striatum of rat brain. Results： All the ratssurvived after operation. The neurologic deficit score of rats in SF group decreased significantly compared with control group(P<0.01). The infarct volume of control group 〔(168.1±42.2)mm3〕 was significant greater than those of SF group〔(61.5±28.7) mm3,P<0.01〕 at 24 hours after reperfusion. The ischemic cortex and striatum in control group showed a markedly increased level of PSD-95 activation and peaked at 30 minutes after reperfusion. Activation of PSD-95 was moderately increased 30 minutes after reperfusion, and peaked at 2 hours, followed by a moderate decline at 6 hours in SF group. Moreover, activation of PSD-95 at each time point in SF group was less than that in control group, and activation of PSD-95 persisted a shorter course in SF group than in control group. No upregulation of PSD-95 protein could be detected in the contralateral cortex. Conclusion： SF could induce neuroprotective effects in transient focal cerebral ischemic injury, weaken PSD-95 activation in ischemic area.
, 百拇医药
PAGE：142-145
Effects and mechanisms of panaxadiol saponins(人参二醇组皂甙) promoting the proliferation of scathing cultured human renal tubular cells
PAN Qiang-wen, RAN Bing, FENG Zhi-qiang, GUO Yong, WANG Qiong
Laboratory of Renal Function of Luzhou Medical College, Luzhou 646000, Sichuan,China.
Abstract： Objective ： To study the effects and mechanisms of panaxadiol saponins(PDS, 人参二醇组皂甙) promoting the proliferation of normally cultured human renal tubular cells and the cells which suffered by the rabbit serum of acute renal ischemic-reperfusion. Methods： The bilateral renal arteries of rabbits were occluded then reperfusion and the serum (SIR) were acquired. The rabbit serums of control (SC) were acquired in the same time. The proliferative activity of cells was measured with the method of MTT. And the rates of living cells were counted. Results： The value of optical density(OD, as A) in 10 mg/L PDS group (0.62766±0.0392) was significant higher than that in control groups (0.5075±0.0597)， P<0.05. The value of A and the rate of living cells in SIR group (0.1989±0.0313 and 46.0%) were significant lower than those in other groups (0.6594-0.7850 and 91.5%-98.9%). But there were not significant differences in the proliferative activity of PDS +SIR groups (0.6594±0.1678) and control group (0.7350±0.0812). Conclusion： Moderate panaxadiol saponins could accelerate the proliferation of normally cultured human renal tubular cells and the cells which suffered by the rabbit serum of acute renal ischemic-reperfusion.
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PAGE：146-148
Effects of ginkgo biloba extract(银杏叶提取物) on platelet activating factor in rats with nephrotoxic serum glomerulonephritis
LIAN Xi-yan, YANG Ling, CHEN Qing, SHEN An-bei, ZHAO Jin-tao
Departmentof Nephrology, the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan, China.
Abstract：Objective： To investigate the effect of ginkgo biloba extract(银杏叶提取物) on platelet activating factor(PAF) and the protection on renal function in rat with nephrotoxic serum glomerulonephritis (as Masugi glomerulonephritis). Methods： The rat model of Masugi glomerulonephritis was induced by Vasslli's methods. Twenty-four animals were randomly divided into the model group, treatment group and non-treated control group. The rats were treated with ginkgo biloba extract. PAF and proteinuria in urine were measured before and after treatment. PAF in serum, renal cortex and renal medulla as well as serum creatinine were also measured after treatment. The effect of ginkgo biloba extract on the renal histopathology in this model was observed. Results：Compared to the model group, ginkgo biloba extract could significantly decrease proteinuria and PAF in urine of 24 hours (P<0.05), serum(P0.05), the renal cortex (P<0.05) and renal medulla, especially in the renal cortex.Prominent histopathological changes and improvement of renal function (P<0.01) were observed in treatment group. Conclusion： Ginkgo biloba extract can inhibit PAF level and has beneficial effects on Masugi glomerulonephritis.
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PAGE：149-151
Study of Xuezhongxiao(血肿消) on pre-protection of kidney in hypertensive cerebral hemorrhage
WANG Yu-qin, WANG Zhen-yu, WANG Ai-feng, WANG Song-ling
Henan Traditional Chinese Medical Hospital, Zhengzhou 450002, Henan, China.
Abstract：Objective： To study the pre-protective effects of integrated traditional Chinese and western medicine on kidney in hypertensive cerebral hemorrhage. Methods： Ninety hypertensive cerebral hemorrhagic patients were randomly divided into three groups： Xuezhongxiao (血肿消) combined with dopamine treatment group(n=30), dopamine treatment group(n=30) and medical treatment group(n=30). The same manitol treatment (250 ml, 1 time per 6 hours) and conventional therapy were used in the three groups. The clinical effects, renal function before and after treatment, absorption of cerebral haematoma and the incidence rate of renal injury were observed in the three groups. Results： The clinical effects and incidence rate of renal injury were significantly different among the three groups (all P<0.05). After treatment, the curative effects in Xuezhongxiao combined with dopamine treatment group was superior to those in dopamine treatment group 〔the total curative effect： 96.67% vs. 90.00%; blood urea nitrogen: (5.72±0.58) mmol/L vs. (6.90±5.35) mmol/L; serum creatinine： (91.00±20.11) μmol/L vs. (98.31±37.66) μmol/L; incidence rate of kidney injury： 3.33% vs. 6.67%; mortality： 0 vs. 0〕, and those in dopamine treatment group were superior to those in medical treatment group 〔63.33%, (7.80±3.67)mmol/L, (148.62±42.02)μmol/L, 26.66% and 6.67% respectively〕. The absorption of haematoma was (95.60±16.21)%, (87.10±12.70)% and (76.35±14.36)% in three groups respectively(all P<0.05). Conclusion： Xuezhongxiao can prevent renal injury induced by manitol, it can quicken absorption of haematoma, raise curative rate and reduce mortality. The curative effect of Xuezhongxiao combined with dopamine is the best in three groups.
, 百拇医药
PAGE：152-154
Clinical research of treatment with high-dose intravenous γ-globulin combined low dose corticosteriod in severe idiopathic thrombocytopenic purpura
FANG Bing-mu, LIU Ying, LI Lin-jie, LIU Wei-hong, JIN Yang-jin
Department of Hematology, Zhejiang Lishui City Central Hospital, Fifth Affiliated Hospital of Wenzhou Medical College, Lishui 323000, Zhejiang, China.
, 百拇医药
Abstract： Objective ： To evaluate the effectiveness of high-dose intravenous γ-globulin (HDIG) combine with low dose corticosteroid in severe idiopathic thrombocytopenic purpura (ITP). Methods： A 6-year retrospective study of 75 patients hospitalized with severe ITP was conducted to compare the effectiveness of corticosteroid and HDIG. HDIG group was given γ-globulin 0.4 g·kg-1·d-1 for 5 days, and prednisolone 0.5 mg· kg-1·d-1 for 28 days. Corticosteroid group was given prednisolone 1-2 mg·kg-1·d-1 for 28 days. Results： In HDIG group, 25 (65.8%) cases were markedly improved, 9 cases (23.7%) were more improved, 2 cases (5.3%) were improved, 2 cases (5.3%) showed no effect, the total effective rate was 94.7%, and they were 23 cases (62.2%), 9 cases (24.3%), 3 cases (8.1%), 2 cases (5.4%) and 94.6% in corticosteroid group. Blood platelet count(BPC) increased significantly after treatment in both two groups compared to those before treatment (both P<0.01), and BPC-peak values in the HDIG group〔(212.56±90.25)×109/L〕 were higher than that in the corticosteroid group 〔(127.26±81.26)×109/L〕. After treatment the time of getting BPC-peak value in the HDIG group〔(7.80±4.50) d〕 was shorter than that in corticosteroid group〔(27.00±9.32)d〕. Conclusion： HDIG combined low dose corticosteroid is more effectively in increasing the platelet count rapidly to safe levels in severe ITP.
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PAGE：155-157
Effect of Xiaoke Antang prescription(消渴安糖方) on the levels of gastrointestinal hormones in patients with type 2 diabetes mellitus of Qi-Yin deficiency syndrome(气阴两虚证)
LIN Shou-ning, LIU Peng, ZHOU Zhuo-ning, WEI Xiao-hong, TAN Chi.
The Second Affiliated Hospital, Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi, China.
Abstract ： Objective： To study the effect of Xiaoke Antang prescription (消渴安糖方) on the serum motilin (MTL), pancreatic glucagons (PG) and substance P (SP) in patients with type 2 diabetes mellitus (DM) of Qi-Yin deficiency syndrome (气阴两虚证), and to observe its therapeutic effects on patients with Qi-Yin deficiency syndrome in diabetic neuropathy. Methods： Ninety cases with type 2 DM of Qi-Yin deficiency syndrome were randomly divided into observation group and control group, 30 healthy people were selected as control. In addition to regular therapy of DM, the observation group was additionally treated with XiaokeAntang prescription. The two groups were observed for a month. MTL, PG and SP were measured by radioimmunoassay before and after treatment, fasting blood sugar (FBS) and 2 hours postprandial blood sugar (PBS) were also measured. During the treatment the syndrome score of gastrointestinal function disorder were observed. MTL, PG and SP of the healthy control group were tested only one time. Results： The levels of MTL and PG in type 2 DM patients of the two groups were higher than the healthy normal group, but the level of SP was lower. There were significant differences between the levels of three above hormones of the two observation groups and that of the normal control group(all P<0.01), and there were great improvement in the abnormal of MTL, PG, SP and in the syndromes of gastrointestinal function disorder after treatment compared with those before treatment (all P<0.01). The improvement of blood sugar(BS) of the observation group was superior to that of the control group(all P<0.05). Conclusion： Xiaoke Antang prescription can betterly improve the abnormal of MTL, PG and SP of Qi-Yin deficiency syndrome in patients with type 2 DM, it proves that Qi-Yin deficiency syndrome in type 2 DM has relativity with the abnormal of MTL, PG and SP. Xiaoke Antang prescription can provide better therapeutic effects on Qi-Yin deficiency syndrome in patients with diabetic neuropathy, and has certainly auxiliary effect on reducing the level of BS.
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PAGE：158-160
Relation between pathogenesis of traditional Chinese medicine and hepatitis B virus-DNA or T lymphocytes in chronic hepatitis B
YANG Hong-zhi, BIAN Zhuang, WANG Yong-ze, GUAN Wei-bing, YANG Yue-wu
The Third Hospital Affiliated Sun Yat-sen University, Guangzhou 510630, Guangdong, China.
Abstract： Objective ：To provide objective indexes for clinical syndrome differentiation in patients with chronic hepatitis B, and research the relation between pathogenesis and hepatitis B virus (HBV-DNA) or T lymphocytes. Methods： The eligible patients with slight and moderate chronic hepatitis B, HBeAg-positive and detectable copy of HBV-DNA in serum were divided into sthenia syndrome (实证, SS) group, intermingled asthenia and sthenia syndrome (虚实兼夹证, IASS) group and asthenia syndrome (虚证, AS) group. The indexes were analyzed in research groups. Results： Compared to controls, level of CD 4 cell in patients with chronic hepatitis B decreased, while there was a significant difference in level of CD+4/CD+8 ratio. The levels of HBV-DNA were SS>IASS>AS(P<0.05). The level of CD+4/CD+8 ratio was AS>IASS>SS(P<0.05). Conclusion： It is suggested that there is disorder in immune regulation in hepatitis B patients. The levels of HBV-DNA could be regarded as the index of SS. The proportion of CD+8 could be regarded as the index of AS. The value of CD+4/CD+8 could be regarded as the index of IASS.
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PAGE：161-163
Effects of Xiaoxintong capsule(消心痛胶囊) on electrocardiogram segment ST and wave T of rats with acute myocardial ischemia
TENG Jiu-xiang, CHEN Xiao-yang, JIANG Meng-liang, PENG Zhi-pei, HAN Guo-dong, SUN Gui-Xiang
Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract： Objective： To investigate effects of Xiaoxintong capsule (消心痛胶囊) on electrocardiogram(ECG) segment ST, elevation of wave T, time and incidence of segment ST and wave T in excess of 0.2 mV. Methods： Rat's acute myocardial infarction (AMI) model was built by using the Pituitrin of hypophysis cerebri. Segment ST and wave T of ECGⅡ were observed as the indexes of the AMI improvement in the medical group. The effects of Xiaoxintong capsule and Diao Xinxuekang capsule(地奥心血康胶囊) were observed. Results： Compared to model group, Xiaoxintong capsule could greatly improve the elevation of segment ST and wave T of the ischemia(P<0.05), reduce the incidence and the time for elevation(both P<0.05). The effect was almost the same between high dosage of Xiaoxintong capsule group and Diao Xinxuekang capsule group. At different time slot, compared to the model group, high dosage of Xiaoxintong capsule group had an obvious significance within one minute (all P<0.01). It could significantly descend the elevation of segment ST and wave T caused by Pituitrin of hypophysis cerebri. Conclusion： Xiaoxintong capsule has good preventive and therapeutic effects of anti-myocardial ischemia. It maybe associate with the function of expanding coronary artery, enhancing blood circulation, improving the blood supply of the heart muscle and its endurance of the oxygen deficiency and regulating the balance of the whole organism.
, 百拇医药
PAGE：164-166
Effect of Feixin mixture(肺心合剂) on monocrotaline induced pulmonary hypertension in rats
YANG Mao-nong, WANG Fei, XU Fang-yuan
The People's Hospital of Sichuan Province, Chengdu 610072, Sichuan, China.
Abstract： Objective： To explore the effect of Feixin mixture(FXM, 肺心合剂) on pulmonary hypertension (PAHT). Methods： Adult male Wistar rats were given a single dose of subcutaneous monocrotaline(MCT, 50 mg/kg) to induce the model of pulmonary hypertension. Sixty cases were randomly divided into normal contral group, PAHT mold group, FXM group and nifedipine group (n=10). Then the rats were treated with gastric infusion of normal saline, FXM or nifedipine in 14th days respectively. The parameters of pulmonary hemodynamics were monitored by Gould3400 polygraph system through the cannulation of polyvinyl tube. Right ventricle and left ventricle added with interventricular septum weight ratio, the lung wet weight and body weight ratio were measured. Results： FXM and nifedipine significantly inhibited the progression of pulmonary artery pressure and decreased the right heart index and reduced the seepage out of the lung (P<0.05 or P<0.01). The effect of high dose FXM group was better. Conclusion： FXM is able to reduce the pulmonary hypertension and improved the heart function.
, 百拇医药
PAGE：167-169
Effect of Xinlening concentrates(心乐宁浓缩液) on hemodynamics in dog with heart failure
ZHI Li-qin, PEI Ji-wen, LI Xi-kuan
Department of Cardiology, Traditional Chinese Medicine Hospital, Xi'an 710001, Shanxi, China.
Abstract： Objective： To investigate the influence of Xinlening concentrates (心乐宁浓缩液) on hemo dynamics in dogs with failure heart. Methods： The experimental dogs were randomly divided into six groups. Negative control group was infused with normal saline, heart failure group was infused with normal saline, and different doses of Xinlening concentrates groups were given Xinlening concentrates 16 g/kg, 32 g/kg, and 64 g/kg respectively. Positive control group was treated with digoxin. The cardiac output was measured with the magnetic volumetric meter at the root of aorta. The inner pressure of left ventricle and the systemic artery pressure were measured by insertion of catheter into carotid and femoral artery respectively. Other hemodynamic indexes were also calculated by confirmed formula. Results： A different dose of Xinlening concentrates and digoxin could mildly increase the average artery pressures. The canine cardiac output could be improved dramatically by different dosage of Xinlening concentrate, the effect of increasing cardiac output of digoxin was not significant different(P>0.05). The inner pressure of left ventricle and the working indexes were also increased with the treatment of different doses of Xinlening concentrates, similar to that of digoxin (P<0.05). The effect of large dosage of Xinlening concentrate was similar to digoxin. Conclusion： The Xinlening concentrates may improve the cardiac output and left ventricular working indexes, improve the hemodynamic status of the canines with failure heart.
, 百拇医药
PAGE：170-173
Experimental research on the effect of E-bing Shuguan drop-pill(鹅冰舒冠滴丸) on arterial lipid plague
CHENG Chou-fu, TAN Yuan-sheng, TAN Sheng-e, JIN Chao-hui. The First Affiliated Hospital, Hunan College of Traditional Chinese Medicine, Changsha 410007, Hunan, China.
Abstract： Objective： To investigate the mechanism of E-bing Shuguan drop-pill (鹅冰舒冠滴丸) on arterial lipid plague in treating coronary angina pectoris. Methods： The hyperlipemia and atherosclerosis rabbit model was established with the phago-source, and the Pituitrin was given by intravenous injection to induce the acute myocardial ischemia. The animals were randomly divided into negative control group, model group, composite salvia miltiorrhiza group and low-, middle-, high-dose of E-bing Shuguan drop-pill group. The biochemistry indexes and pathological histology changes were observed. Results： E-bing Shuguan drop-pills could regulate the blood lipid(high-dose of it had the best effect) and the balance of endothelin (ET)/nitric oxide(NO), increasing high-density lipoprotein cholesterol(compared to the composite salvia miltiorrhiza group, P<0.05), protect the endothelial cell, improve myocardial ischemia of the experimental rabbit. High-dose group had the better effect on decreasing the increased T wave at 30 minutes compared to the composite salvia miltiorrhiza group (P<0.05). E-bing Shuguan drop-pill could decrease the size of arterial lipid plague, especially in middle-and small-dose group(compared to the composite salvia miltiorrhiza group, both P<0.05). Conclusion： E-Bing Shuguan drop-pills can protect myocardial, delay atherosclerosis lipid plaque happening and developing, even extincting atherosclerosis lipid plaque.
, 百拇医药
PAGE：174-176
Study on treatment of upper digestive tract hemorrhage by Huangmi starch(黄蜜浆) and proton pump inhibitor
LIN Ping，LIAN Ming-feng，ZHANG Qiang，ZHANG Lie-hu，YANG Jian-min
The First Hospital of Longyan, Longyan 364000, Fujian, China.
Abstract： Objective： To investigate the therapeutic value and application regularity on treatment of upper digestive tract hemorrhage with traditional Chinese and western medicine Huangmi starch(黄蜜浆) and proton pump inhibitor. Methods：One hundred and thirty patients with upper digestive tract hemorrhage were randomly divided into three groups，42 patients were treated with Huangmi starch， 42 patients were treated with proton pump inhibitor，and 46 patients were treated combined with Huangmi starch and proton pump inhibitor.The therapeutic efficacy and hemostatic time were observed and compared in three groups. Results： The fully recovery rate in patients treated by Huangmi starch and proton pump inhibitor were highter than treated by proton pump inhibitor(87.0% vs.69.0%， P<0.05)，hemostatic time in patients treated by Huangmi starch and proton pump inhibitor were better than only Huangmi starch or proton pump inhibitor groups 〔the time of tarry stood turned yellow and stood occult blood disappeared: (36.5±10.2) h,(2.5±0.9) d vs.(52.3±15.6)h,(3.9±1.2)d, and (81.4±25.5)h,(5.2±1.9)d, all P<0.01〕. Conclusion：Treatment of upper digestive tract hemorrhage combine with Huangmi starch and proton pump inhibitor can shorten hemostatic time，increase therapeutic efficacy. Huangmi starch and proton pump inhibitor have synergistic action.
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PAGE：177-180
Management of acute postoperative bleeding after pancreatoduodenectomy
JIANG Kai, ZHOU Ning-xin, HUANG Zhi-qiang, LIU Rong, HUANG Xiao-qiang, ZHANG Wen-zhi, WANG Mao-qiang
The General Hospital of People's Liberation Army, Beijing 100853, China.
Abstract: Objective: To investigate the management principle and method for the acute postoperative bleeding after pancreatoduodenectomy(PDT). Methods: The experience of six patients with hemorrhage after pancreatoduodenectomy (PDT) in our hospital from 1991 to 2001 were summarized, and some literatures were reviewed for analyzing the clinical presentation, bleeding reason, management and outcome. Results: Sudden severe super abdomen pain and in/out- drainage tube bleeding of peritoneal cavity were the main manifestation for all patients, and combining supper gastrointestinal tract bleeding for two of them. All patients had pancreatic leak. Emergency arterial angiography examination was made for all cases to confirm reason of the bleeding from gastroduodenal artery (3 cases), inferior pancreatoduodenal artery (2 cases) and posterior edge of pancreatic stump (1 case), respectively. The former (3 cases) were treated by hepatic arterial embolization without rebleeding, and the latter (3 cases) were undergone by relaparotomy hemostasis. One patient died of multiple organ failure because of repeat postoperative rebleeding after 5 days. Intestine function could restore after treatment with traditional Chinese herbs of strengthening spleen and activating Qi (健脾行气). Conclusion: Necessary preoperative preparation and emergency arterial angiography should be done rapidly for all acute hemorrhage of the patients to confirm the bleeding source. Selecting hepatic aterial embolization or operation hemostasis is depended on the bleeding position.
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PAGE：181-182
Clinical study of naloxone of severe craniocerebral trauma with both corectasis
MIAO Yu-lu, XI Yin-xue, JIANG Zheng-ming, NIE Yu-lin, YU Shao-xiong, YAO Li-zhi, ZOU Shi-cai, FU Hong-jin, HUANG Zhi-bin
Buji People's Hospital, Shenzhen 518112, Guangdong, China.
Abstract： Objective： To assess clinical effects of naloxone on severe craniocerebral trauma with both corectasis. Methods： Patients (GCS were 3-8 scores) with severe craniocerebral trauma were randomly divided into two groups： treatment group and control group. Control group was treated by oxygen uptake, compression of cranium, dehydration, using antibiotic, keeping nutritive equilibrium, tracheostomy, etc. Treatment group was treated with naloxone except for control group. Observation indexes： 24 hours electrocardiograph, blood gas analysis, degree of blood oxygen saturation; breath and heart rate after 7-day treatment, hemorrhage of digestive tract after 10- day treatment, cerebral infarction, mortality, linguistic and move function after 3- week treatment. Results： Compared to the control group, the time of recovery coma was shorter 〔(10.61±4.11) days vs.(15.78±3.95) days〕, heart rate, breath rate, imbalance of acid and basic, the incidence of digestive tract hemorrhage were improved, the incidence of cerebral infarction, linguistic, and mortality were decreased (P<0.05 or P<0.01). Conclusion： Naloxone treating severe craniocerebral trauma with both corectasis have good clinical effects, significantly reduce mortality and disability, improve the recovery of coma.
, 百拇医药
PAGE：183-185
Evaluation of randomized controlled trials in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi
XUE Xin-sheng, ZHANG Zhong-wei, ZHOU Yan
ICU, Huaxi Hospital of Sichuan University, Chengdu 610041, Sichuan, China
Abstract： Objective： To evaluate the quantity of the randomized controlled trials (RCTs) papers published in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi and offer the basic information to Chinese Cochrane center. Methods： The control trials of clinical therapeutic studies in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi from 1994 to 2001 were checked to identify strictly the RCT and controlled clinical tests (CCTs) according to the criteria of the handbook of Cochrane collaboration in 1997. Results： One thousand six hundred and six papers on clinical trials were published in 67 issues covered 8 volumes of Zhongguo Zhongxiyi Jiehe Jijiu Zazhi. Among them, there were 618 papers of RCT (38.48%), 203 papers of CCT (12.64%), and the papers of RCT increased more significantly, especially after 1998, and it was 60.32% in 2000.Conclusion： The quality of RCT papers published in Zhongguo Zhongxiyi Jiehe Jijiu Zazhi has been improved year by year., 百拇医药