Chin Crit Care Med, Aug 2004,Vol.16,No 10
PAGE: 577-578
Study on the method of first critical care on spot disaster shoaled be strengthened
YUE Mao-xing
Center for special medicine of 306th Hospital of the PLA, Beijing, 100101, China
PAGE: 579-581
Comment on contentions problewm of multiple organ dysfunction syndrime
Jing Bing-wen
, http://www.100md.com
Department of Emergency, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
PAGE: 582-584
Application of Glucocorticoid in the Treatment of Septic shock
Yang Yi
ICU, Zhongda Hospital, South-east University Nanjing 210009, Jiangsu, China
PAGE: 585-588
, 百拇医药
Studies on the expression of mRNA of anit- and pro-inflammatory cytokines in acute lung injury induced by lipopolysaccharide in rat
Zhang Qing, Li Qi, Mao Bao-ling, Qian Gui-sheng, Xu Jian-cheng, Chen Zheng-tang
Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Objective: To study the role of pro-inflammatory cytokines including tumor necrosis factor-α(TNF-α)， interleukin-1β(IL-1β)，IL-6， and anti-inflammatory cytokines including IL-4， IL-10， and IL-13 in acute lung injury(ALI). Methods: Reverse transcription-polymerase chain reaction(RT-PCR) were used to assess the expressions of mRNA of TNF-α, IL-1β, IL-6, IL-4, IL-10 and IL-13 in the lung tissue after lipopolysaccharide challenge. Results: The expression of TNF-α mRNA was seen to peak at 1 hour and IL-6 mRNA at 4 hours in lung tissue after the challenge. The highest expression of mRNA of other cytokines, such as IL-1β, IL-4, IL-10 and IL-13 in lung tissue appeared 2 hours after LPS-induced lung injury. Conclusion: TNF-α is an early presenting cytokine in ALI, and IL-6 may play an important role in delayed development of ALI. The over-expression of anti-inflammatory cytokines IL-4, IL-10 and IL-13 may act as promoters rather than protectors in amplification of inflammatory process.
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PAGE: 588
Comparison of First Aid Between Tianjin and Singapore
WANG Tao
The Second Teaching Hospital of Tianjin College of Traditional Chinese Medicine, Tianjin 300051, China
PAGE: 589-591
Establishment of a mobile intensive care unit
YUE Mao-xing, ZOU De-wei， ZHANG Jian, LIU Zhi-guo, CUI Shao-jie, FANG Wei-wu, ZHOU Xue-feng, GAO Tie-shan, HUA Nan.
, 百拇医药
Center for Special Medicine of 306th Hospital of the PLA, Beijing 100101, China
Objective: To discuss the experience of establishment of a mobile intensive care unit(ICU) for emergency treatment following astronaut flight, disaster, and regional war. Methods: The data from both foreign and our countries as well as our own clinical experience of first-aid were reviewed, and a mobile ICU was established, including the equipment, supply of drugs, training, and organization. Results: The mobile ICU was set up at the site of landing of the first Chinese astronaut, and proved to be efficient. The new model could be expected to be prompt in first-aid for casualties in disasters and warfare. Conclusion: The first-aid on spot of casualties might reduce the rates of invalidity and mortality during disasters and regional war.
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PAGE: 592-595
High mobility group box-1 protein activates Janus kinase-signal transducer and activator of transcription pathway in rat peritoneal macrophages
LIU Hui, YAO Yong-ming, DONG Yue-qing, YU Yan, SHENG Zhi-yong.
Burns Institute, 304th Hospital of PLA, Beijing 100037, China
Objective: To investigate the potential signal transduction mechanism in high mobility group box-1 protein (HMGB1)-induced inflammatory response in rat peritoneal macrophages. Methods:Peritoneal macrophages obtained from male Wistar rats were incubated for 3 days before they were stimulated by HMGB1 (10 μg/ml). At various time points after HMGB1 stimulation, macrophages were denatured directly in cell culture flasks to detect activation of Janus kinase-2 (JAK2), signal transducer and activator of transcription-1 (STAT1) and STAT3 by immunoprecipitation, Western blotting and electrophoretic mobility shift assay, respectively. Results: HMGB1 stimulation could activate STAT1 and STAT3 in peritoneal macrophages in 2 hours, among them the activation of STAT3 appeared to be the quickest, peaking as early as 10 minutes after stimulation. But no marked change in JAK2 activity was observed within 2 hours following HMGB1 stimulation. Conclusion: These data suggest that Janus kinase-signal transducer and activator of transcription pathway might be involved in regulation of HMGB1-induced inflammatory response in peritoneal macrophages.
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PAGE: 595
Analysis of Clinical Characteristic of 8 SARS Accompany with Upper alimontary tract hemorrhages
Wang Shuo
Beijing Chaoyang Hospital, Capital University of Medical Science, Beijing 100020, China
PAGE: 596-598
Effect of α-melanocyte stimulating hormone on the apoptosis of the vascular endothelial cell of the lung in two-hit acute respiratory distress syndrome in rat
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MIAO Yu-liang , DENG Xiao-ming, LI Jin-bao, ZHAO Xiang-kun, CAO Jin-mei， TIAN Ye-ping.
Department of Anaesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
Objective: To investigate the effect of α -melanocyte stimulating hormone (α-MSH) on the apoptosis of the vascular endothelial cells of the lung in acute respiratory distress syndrome(ARDS) reproduced with acute hemorrhagic shock followed by intratracheal lipopolysaccharide(LPS,two-hit model) in rat.Methods: Ten male Sprague Dawley rats, weighing (33.7±2.5)g, were randomly divided into two groups(A and B) with 5 in each group. All rats were anesthesized and ventilated mechanically with fractional concentration of inspired oxygen (FiO 2) of 0.5, breath rate 100 times/min, tidal volume(V T) 12 ml/kg and inspiratory/expiratory ratio (I/E) 1∶15. The blood was withdrawn to induce hemorrhagic shock via the carotid artery until blood pressure reached (45±5)mm Hg(1 mm Hg=0.133 kPa), which was maintained for 1 hour, and the shed blood and Ringer's lactate in volume equal to the shed blood were reinfused in 2 hours for resuscitation. Afterwards, LPS was given via the tracheal (200 μg/kg，in 500 μl normal saline) to establish the ARDS model. Group A was ARDS control group, group B was α-MSH administration group. α-MSH was intravenously administrated simultaneously, 3 hours and 6 hours after LPS given, the dosage was 17 mg/kg at each time point. The rats were sacrificed at 9 hours after LPS challenge, and the lung tissue was examined with microscope and electron microscope to observe the pathological changes and apoptosis of the vascular endothelial cells. Results: In ARDS control group, remarkable infiltration of inflammatory cells was found in the alveoli, and the apoptosis of the vascular endothelial cells had developed to late stage. In α-MSH treatment group, few inflammatory cells were found in the alveoli, and the apoptosis of the endothelial cells was still in an early stage. Conclusion: α-MSH could inhibit the apoptosis of the vascular endothelial cells of the lung in the two-hit ARDS in rats. Therefore, it might have a protective effect on the lung after hemorrhagic shock and endotoxin challenge.
, 百拇医药
PAGE: 599-602
Value of blinded sampling methods in the pathogenic diagnosis of ventilator-associated pneumonia
CAI Shao-hua ， YU Sen-yang， ZHANG Jin-chuan， LIU Chang-ting， QIAN Gui-sheng.
Department of Geriatric-Respiratory, General Hospital of PLA, Beijing 100853, China
Objective: To evaluate the diagnostic value of blinded protected specimen brush and quantitative culture (BPSB-QC) in the pathogenic diagnosis of ventilator-associated pneumonia (VAP). Methods: A prospective, self-controlled clinical trial was conducted during a 36-month period. QC of paired samples of BPSB and PSB via a fiberoptic bronchoscopy(FOB- PSB) in a total of 54 patients during 125 suspected episodes of VAP was compared. The sensitivity and accuracy, as well as the concordance between BPSB-QC and FOB-PSB-QC result in 48 patients with 106 episodes of VAP were assessed. Both BPSB-QC and FOB-PSB-QC greater than 103 cfu/ml (positive cutoff) was considered diagnostic of VAP. Results: Forty-eight patients with 106 episodes were considered to have VAP (84.8%). The accuracy of BPSB-QC and FOB-PSB-QC were 80.8%(101/125) and 83.2%(104/125), respectively. The rate of complete concordant results was high (75.2%) for BPSB-QC and FOB-PSB-QC. The pathogenic diagnostic agreement between the two techniques was 84.8%(106/125). There were no significant differences with regard to site of pneumonia and positive diagnostic rate between the two techniques. Conclusion: BPSB-QC has similar accuracy and same feasibility compared with FOB-PSB-QC which was commonly primarily used in the pathogenic diagnosis of VAP, and that its use is substantially simpler, safe and cost saving, especially when FOB technique is not available.
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PAGE: 603
Rescue Acute Oranophosphones Pesticides Poisoning Muscles, Respiratory Parlysis Three Key Links
LUO Qing-yi, SUN Hong-wei
ICU Cao Country People's Hospital, Shandong 27440, China
PAGE:603-607
Effect of sustained inflation on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome in rabbit
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YANG Yi, QIU Hai-bo, YAN Yan-li, XU Hong-yang, WANG Li, ZHOU Shao-xia, ZHENG Rui-qiang.
Department of Critical Care Medicine, Zhong-da Hospital and School of Clinical Medicine, Southeast University, Nanjing 210009, Jiangsu, China
Objective: To evaluate the influence of sustained inflation (SI) on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome(ARDS) in rabbit. Methods: The ARDS rabbit model were replicated by saline alveoli-lavage. The rabbits were randomly divided into four groups: ①normal group; ②ARDS group; ③low -volume(V T)+best end-expiratory pressure group(LVBP): V T 6 ml/kg, PEEP 2 cm H2O(1 cm H2O =0.098 kPa) greater than the pressure of lower inflection point in pressure-volume curve(Pinf); ④SI group: same settings as LVBP group plus SI performed every hour. After 4 hours of ventilation, liver and intestine homogenates were prepared to assay tumor necrosis factor(TNF)-α and interleukin(IL)-10 levels by enzyme-linked immunoadsorbent assay and their mRNA expression by reverse transcription-polymerase chain reaction. Myeloperoxidase (MPO) and malondialdehyde (MDA) were assessed at the same time. Results: In SI group, TNF-α mRNA expression in the liver and intestine tissues (24.82±7.88 and 25.06±10.57) were decreased significantly compared with ARDS(36.88±6.83 and 36.48±8.23) and LVBP groups (42.34±8.55 and 37.59±10.63, all P<0.05). The expression of IL-10 mRNA in the liver and intestine of rabbits in SI group(17.31±5.72 and 10.90±3.30) was lower than that of ARDS(24.64±3.85 and 24.31±7.69) and LVBP group(25.03±9.38 and 21.83±7.34, all P<0.05) signifantly. In SI group, the levels of TNF-α and IL-10 in liver and intestine tissue were similar to that of the other groups. The contents of MPO and MDA had no difference compared with the other groups too. Conclusion: SI can down-regulate inflammatory mediator expression of extra-pulmonary organs in rabbits with ARDS, and it may inhibit the inflammatory response in extra pulmonary organs.
, 百拇医药
PAGE:607
Clinical Efficacy on Na-DMPS Combined with Vecuronium in Emergency Treatment of Acute Tetramine Intoxication
Zhang Yong-tian, Tian Yong-qiang, Wang Wei-cheng, Kang Le-chang, Guo Chang-xing
Department of Emergency, Xiji People's Hospital, Ningxia 756200, China
PAGE: 608-610
Inflammatory injurious effect of angiotensin Ⅱ on pulmonary microvascular endothelium in rat
, 百拇医药
ZHANG Hong, SUN Geng-yun.
Department of Emergency, First Affiliated Hospital, Anhui Medical University, Hefei 230061, Anhui, China
Objective: To investigate the effects of angiotensin Ⅱ (AngⅡ) and its receptors on monolayer permeability of pulmonary microvascular endothelial cells in rat. Methods: The following examinations were done on cultured rat pulmonary microvascular endothelial cells (RPMVECs). ①Micro-infiltrator was used to assay the variations of lipopolysaccharide(LPS)-induced increased RPMVECs monolayer permeability coefficient (Kf) in different periods. ②Effect of angiotensin Ⅱ on LPS-induced permeability injury to the endothelium, and preventive effects of angiotensin Ⅱ type 1 receptor antagonist (Sar1, Ile8)-Ang Ⅱ. Results: LPS increased RPMVECs monolayer permeability compared with normal control, and AngⅡ exacerbated LPS-induced RPMVECs monolayer permeability significantly. This synergistic effect was significantly prevented by the addition of (Sar1, Ile8 )-Ang Ⅱ. Conclusion: AngⅡ and LPS have synergistic injurious effects to the pulmonary microvascular endothelium in rat, and AngⅡ exacerbates increase in LPS-induced RPMVECs monolayer permeability. This synergistic effect is significantly prevented by the addition of (Sar1, Ile8)-Ang Ⅱ.
, 百拇医药
PAGE:611-614
Protective effect of N-acetylcysteine on acute lung injury caused by exposure to rocket liquid propellant
LIU Song, YU Sen-yang. Department of Respiratory Diseases， General Hospital of PLA, Beijing 100853, China
Objective: To investigate the protective effect and its mechanisms of N-acetylcysteine (NAC) on acute lung injury (ALI) caused by exposure to high concentration rocket liquid propellants asymmetrical dimethylhydrazine (UDMH) and dinitrogen tetroxide (N 2O 4). Methods: Forty-two rats were randomly divided into three groups: the control group, the exposure group and the exposure plus the treatment group (NAC group). The rats of the latter two groups were exposed to UDMH 098 mg/L for 10 minutes and then N 2O 4 0.19 mg/L for another 10 minutes. After the exposure, the NAC group rats received immediately 150 mg/kg of NAC intravenously, and rein forced by intraperitoneal injection of NAC with a dose of 50 mg/kg 3 hours after the intravenous injection. The rats of other group were treated with saline in equal volume. All rats were killed after 6 hours. The lung wet to dry ratio (W/D ), the contents of lactate dehydrogenase (LDH) and total protein in bronchoalveolar lavage fluid (BALF), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in lung tissue, and the malondialdehyde (MDA) of plasma were measured. Pathological examination was performed. Results: The lung W/D ratio, the LDH and total protein in BALF, and the MDA of plasma were increased in the exposure group, while the activities of SOD and GSH-Px in lung tissue were decreased. The histopathology of the rats of exposure groups showed that there was exudation within alveolar spaces and prominant interstitial thickening of septa. In the NAC group, the values of the above findings were lowered, and the degree of lung injury was alleviated in histopathology. The lung W/D were negatively correlated with the activities of SOD and GSH-Px in lung tissue, and the correlation coefficient were-0662 (P<0.01) and -0707(P<0.01) respectively. Conclusion: The administration of NAC appears to attenuate the injury to the lung after an exposure to UDMH and N 2O 4 in high concentration, and the antioxidant activity of NAC may be responsible for the protective effect.
, 百拇医药
PAGE: 614
Management of acute abdomen caused by cholecystoenteral fistula, one case report of acute perforation of cholecystoduodenal fistula with carcinoma of rectum
LIU Chun, SHAN Dan
Department of General Surgery, Tianhe Hospital, Tianjin 300050, China
PAGE: 615-617
Clinical investigation of application of lung biopsy in the diagnosis of diffuse pulmonary interstitial disease
, 百拇医药
GAO Zhan-cheng, MA Mei-xing, GU Li-xin, LIU Jun, WANG Jun, SHEN Dan-hua.
Departments of Respiratory Medicine, Peking University, People's Hospital, Beijing
100044, China
Objective: To evaluate the application of lung biopsy in the diagnosis of diffuse pulmonary interstitial disease (DPID). Methods: The data of lung biopsy from 17 cases with uncertain DPID obtained during the period of July 1997 and December 2002 were retrospectively analysed. Results: All the cases were diagnosed by pathology. Three of them were diagnosed as sarcoidosis. Two were pulmonary alveolar proteinosis. One with usual interstitial pneumonia (UIP), one with bronchialitis obliterous and organizing pneumonia, one with nonspecific pneumonia, one with Castleman's disease, one with pulmonary lymph angioleiomyomatosis, two with bronchioalveolar carcinoma, one as rheumatoid pulmonary fibrosis complicated with pulmonary squamatous cancer and locally metastas is in lungs, two as pulmonary lymphangitic carcinomatosis, one as lung squamatous cancer with pulmonary lymphangitic metastasis, and one with primary pulmonary paraganglioma. Conclusion: Harvest of lung tissue directly by biopsy could be the most useful diagnostic method for DPID, and it is a highly valuable diagnostic tool for those patients whose diagnosis is uncertain with routine chest film and high resolution computed tomography.
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PAGE: 617
Application of the Technique of Continuous Blood Purification(CBP) to a case of Tetramine and Multiple Organ Failure(MOF) Complications
WU Xiao-ning, LIU Huan-hao
Nephro-intermal Medicine of the Fifth Affiliated Hospital of Guangxi Medical university, Liu zhou, Guangxi 545001, China
PAGE: 618-621
Study on effect of dexamethasone on the expression of matrix metalloproteinase and its tissue inhibitors in hyperoxia-induced lung injury
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LIU Cheng-jun, XU Feng, KUANG Feng-wu, DANG Run, LU Zhong-yi, WANG Xing-yong.
PICU of Children's Hospital of Chongqing University of Medical Sciences, Chongqing 400014, China
Objective: To observe the effect of dexamethasone on the mRNA expression of matrix metalloproteinase (MMPs) and tissue inhibitor of metalloproteinase(TIMPs) in the lung tissue and to explore the protective mechanism of dexamethasone in hyeroxia-induced lung injury. Methods: Thirty-two two-week old Wistar rats were randomly divided into atmospheric-air group (n=16) and hyperoxia group (n=16). After 7 days of continuous exposure to high concentration O 2(≥95%), the lung wet/dry(W/D) ratio, the protein content in bronchoalveolar lavage fluid(BALF) and histopathological changes of the lung were measured in 16 rats(8 in each group). The lung tissue specimens of the other 16 rats were cultured, 8 among which served as stmospheric-air control group, the remainder in the hyperoxia group were divided into hyperoxia control group,hyperoxia+dexamethasone (1×10-8mol/L) group, hyperoxia+dexamethasone (1×10-6 mol/L) group, and hyperoxia+dexamethasone (1×10-4 mol/L) group. Eight samples were examined in each group. After cultured for 24 hours, the lung tissue were collected and its mRNA expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: ①Pulmonary edema, hemorrhage and extensive inflammatory cells infiltration were observed in hyperoxia group, but no such changes were found in the atmospheric-air group. The lung W/D and the protein content in BALF in hyperoxia group were significantly higher than those in atmospheric air groups. ②The mRNA expressions of MMP-2, MMP-9, TIMP-1, TIMP-2 and the ratio of MMP-2/TIMP-2, MMP-9/TIMP-1 were significantly higher in the hyperoxic group than those in the atmospheric-air group. ③Dexamethasone could down-regulate the mRNA expressions of MMP-2 and MMP-9 in a concentration dependant manner. The mRNA expressions of TIMP-1, TIMP-2 also could be reduced by dexamethasone. Decreasing ratios of MMP-2/TIMP-2 and MMP-9/TIMP-1 were found in correlation with increasing concentration of dexamethasone. Conclusion:: Dexamethasone can reduce the mRNA expressions of MMPs as well as regulate the balance of MMPs/TIMPs, which may be one of the mechanism of its protective effect on hyperoxia-induced lung injury.
, 百拇医药
PAGE: 622-625
Change in Toll-like receptor 4 expression on interstitial macrophages in acute lung injury
ZHANG Wei, JIANG Yao-guang, XIE Zhi-jian, LU Fenglin, HU Cheng-xiang, LI Lei.
Department of Thoracic Surgery, South-West Hospital, Third Military Medical University, Chongqing 400038, China； Corresponding author: LI Lei. First Department, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
, 百拇医药
Objective: To observe changes in the level of Toll-like recepter 4(TLR4) mRNA and the expression of TLR4 protein in interstitial macrophages in serious thoracic injury. Methods: A rat model of severe thoracic trauma was reproduced,then interstitial macrophages were isolated and collected by enzymatic digestion before and after trauma cultured. TLR4 mRNA and the expression of TLR4 protein were measured by Northern and Western blotting before trauma and 2, 4, 8, 16, 24 hours after trauma.Results: A stable and reliable severe thoracic trauma model was successfully reproduced with 400 kPa impact on the up-right chest of rat by a multiple-function collision apparatus. The expression was up-regulated by trauma and the highest expression of interstitial macrophages occurred 8, 16 hours after the trauma, and returned to normal range at 24 hours. Conclusion: The up-regulation of TLR4 might participate in the pathological process of excessive inflammation after trauma to the lung.
, 百拇医药
PAGE:626-628
Study on molecular mechanisms of indirect suppression of albumin mRNA expression of hepatocytes by lipopolysaccharide in rat
WANG Xin-ying, LI Wei-qin, LI Ning, LI Jie-shou . Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu, China
Objective: To explore the molecular mechanisms of relationship between the decreased expression of albumin gene of the cultured hepatocytes by lipopolysaccharide (LPS) and cytokines. Methods: As an inhibitor of protein synthesis, cycloheximide (CHX) could inhibit the LPS-induced synthesis of cytokines. Therefore, the culture system pretreated with CHX could exclude the influence of cytokines on the regulation of albumin gene expression, then it might prove that LPS induced down-regulation of expression of albumin gene was a through cytokine-dependent pathway. The concentrations of interleukin(IL-6) and tumor necrosis factor-α(TNF -α) in the supernatant were also assayed. Results: The expression of albumin mRNA is inhibited significantly by LPS. CHX could block the pathway of LPS-induced down-regulation of expression of albumin gene at least at the transcription level. And the prevention was in a dose-related manner. The increase of IL-6 and TNF-α induced by LPS was inhibited by CHX(both P<0.05). Conclusion: The results suggest that LPS can indirectly inhibit the transcription of albuminogene through cytokines such as IL-6 and TNF-α.
, 百拇医药
PAGE: 629-631
Effects of norepinephrine on the therapeutic effect of nitric oxide inhalation in the treatment of acute respiratory distress syndrome in goat
LIU Shao-hua, XU Xin-rong, XU Ji, ZUO Xiang-rong, QIN Ping-ping.The First Affiliated Hospital of Nanjing Medicial University, Nanjing 210029, Jiangsu, China
Objective: To observe effects of norepinephrine (NE) on the therapeutic effects of nitric oxide (NO) inhalation in goats with endotoxin-induced acute respiratory distress syndrome (ARDS). Methods: A model of septic ARDS was reproduced by an intravenous infusion of low dose endotoxin in six goats, and then these animals were treated with 40×10-6 NO inhalation. After 30 minutes, intravenous infusion of NE in dose of 0.5 ug·kg-1·min-1 was given. The dynamic changes in gas exchange and hemodynamics were measured with the aid of Swan-Ganz catheter and arterial blood gas analysis before and after the onset of ARDS, 30 minutes after NO inhalation and administration of NE. Results: Inhalation of NO rapidly reduced mean pulmonary arterial pressure (MPAP), increased PaO2, decreased alveolar-arterial partial pressure of oxygen difference (P (A-a) O2) and intrapulmonary shunt fraction (Qs/Qt) in septic ARDS goats. These changes were more pronounced when NE was given compared with NO inhalaion alone. The combination of NO inhalation and NE infusion resulted in an increase in mean arterial pressure. Conclusion: Norepinephrine enhances the beneficial effect of nitric oxide inhalation on lung gas exchange in goats with endotoxin induced acute respiratory distress syndrome.
, 百拇医药
PAGE: 632
Applications of hemoperfusiion associated with hemodialysis in renal transplantation
ZHOU Shu-hua, GU Zhen-xiang, ZENG Sheng
Dezhou People‘s Hospital, Dezhou 253014, China
PAGE: 633
Clinic observations on pulmonary tidal volume during closed-chest lift-compression
WANG Li-xiang, WANG Fa-qiang, ZHANG Jian-peng, LIU Hui-liang, LEI Zhi-li, WEI Zhong-yu, XIA Hong-jie, LEI lianl-hui, WANG Yu-gui, ZHANG Jie, HOU Hui-ya
, 百拇医药
ICU, The General Hosptial of the Chinese people’s armed police forces, Beijing 100039, China
PAGE:634-635
The relationship between chlamydia Pneumoniae Infection and Asthma
GUO Qiang, ZHANG Lian-xiang, CAI Zhong
Tianjin Thoracic Hospital, Tianjin 300051, China
PAGE: 635
Hemo-purification in treatment of 6 cases with acute Dushugqiang poisoning
, 百拇医药
YU Qing-chun, WEI Jin-zhi, WEI Hong-zhen, SONG Ying-hua, WU Bin, WANG Yan-xu, Zang Yi-ming
Pingdingshan General Company of Coal Hospital, Henan 467000, China
PAGE: 636-638
The potential mechanisms underlying dissonance of cell-mediated immunity diring sepsis
DONG Yue-qing, YAO Yong-ming
Burns Institute, 304th Hospital of PLA, Beijing 100037, China
PAGE: 639-640
Local rennin-angiotensin system and sepsis
LI Jing-ling, YIN Chen-hong, ZHANG Shu-wen, WANG Bao-en
Department of infection and critical care medicine of (Beijing) friendship hospital affiliate of capital university of medical sciences, 百拇医药
Study on the method of first critical care on spot disaster shoaled be strengthened
YUE Mao-xing
Center for special medicine of 306th Hospital of the PLA, Beijing, 100101, China
PAGE: 579-581
Comment on contentions problewm of multiple organ dysfunction syndrime
Jing Bing-wen
, http://www.100md.com
Department of Emergency, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
PAGE: 582-584
Application of Glucocorticoid in the Treatment of Septic shock
Yang Yi
ICU, Zhongda Hospital, South-east University Nanjing 210009, Jiangsu, China
PAGE: 585-588
, 百拇医药
Studies on the expression of mRNA of anit- and pro-inflammatory cytokines in acute lung injury induced by lipopolysaccharide in rat
Zhang Qing, Li Qi, Mao Bao-ling, Qian Gui-sheng, Xu Jian-cheng, Chen Zheng-tang
Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Objective: To study the role of pro-inflammatory cytokines including tumor necrosis factor-α(TNF-α)， interleukin-1β(IL-1β)，IL-6， and anti-inflammatory cytokines including IL-4， IL-10， and IL-13 in acute lung injury(ALI). Methods: Reverse transcription-polymerase chain reaction(RT-PCR) were used to assess the expressions of mRNA of TNF-α, IL-1β, IL-6, IL-4, IL-10 and IL-13 in the lung tissue after lipopolysaccharide challenge. Results: The expression of TNF-α mRNA was seen to peak at 1 hour and IL-6 mRNA at 4 hours in lung tissue after the challenge. The highest expression of mRNA of other cytokines, such as IL-1β, IL-4, IL-10 and IL-13 in lung tissue appeared 2 hours after LPS-induced lung injury. Conclusion: TNF-α is an early presenting cytokine in ALI, and IL-6 may play an important role in delayed development of ALI. The over-expression of anti-inflammatory cytokines IL-4, IL-10 and IL-13 may act as promoters rather than protectors in amplification of inflammatory process.
, http://www.100md.com
PAGE: 588
Comparison of First Aid Between Tianjin and Singapore
WANG Tao
The Second Teaching Hospital of Tianjin College of Traditional Chinese Medicine, Tianjin 300051, China
PAGE: 589-591
Establishment of a mobile intensive care unit
YUE Mao-xing, ZOU De-wei， ZHANG Jian, LIU Zhi-guo, CUI Shao-jie, FANG Wei-wu, ZHOU Xue-feng, GAO Tie-shan, HUA Nan.
, 百拇医药
Center for Special Medicine of 306th Hospital of the PLA, Beijing 100101, China
Objective: To discuss the experience of establishment of a mobile intensive care unit(ICU) for emergency treatment following astronaut flight, disaster, and regional war. Methods: The data from both foreign and our countries as well as our own clinical experience of first-aid were reviewed, and a mobile ICU was established, including the equipment, supply of drugs, training, and organization. Results: The mobile ICU was set up at the site of landing of the first Chinese astronaut, and proved to be efficient. The new model could be expected to be prompt in first-aid for casualties in disasters and warfare. Conclusion: The first-aid on spot of casualties might reduce the rates of invalidity and mortality during disasters and regional war.
, http://www.100md.com
PAGE: 592-595
High mobility group box-1 protein activates Janus kinase-signal transducer and activator of transcription pathway in rat peritoneal macrophages
LIU Hui, YAO Yong-ming, DONG Yue-qing, YU Yan, SHENG Zhi-yong.
Burns Institute, 304th Hospital of PLA, Beijing 100037, China
Objective: To investigate the potential signal transduction mechanism in high mobility group box-1 protein (HMGB1)-induced inflammatory response in rat peritoneal macrophages. Methods:Peritoneal macrophages obtained from male Wistar rats were incubated for 3 days before they were stimulated by HMGB1 (10 μg/ml). At various time points after HMGB1 stimulation, macrophages were denatured directly in cell culture flasks to detect activation of Janus kinase-2 (JAK2), signal transducer and activator of transcription-1 (STAT1) and STAT3 by immunoprecipitation, Western blotting and electrophoretic mobility shift assay, respectively. Results: HMGB1 stimulation could activate STAT1 and STAT3 in peritoneal macrophages in 2 hours, among them the activation of STAT3 appeared to be the quickest, peaking as early as 10 minutes after stimulation. But no marked change in JAK2 activity was observed within 2 hours following HMGB1 stimulation. Conclusion: These data suggest that Janus kinase-signal transducer and activator of transcription pathway might be involved in regulation of HMGB1-induced inflammatory response in peritoneal macrophages.
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PAGE: 595
Analysis of Clinical Characteristic of 8 SARS Accompany with Upper alimontary tract hemorrhages
Wang Shuo
Beijing Chaoyang Hospital, Capital University of Medical Science, Beijing 100020, China
PAGE: 596-598
Effect of α-melanocyte stimulating hormone on the apoptosis of the vascular endothelial cell of the lung in two-hit acute respiratory distress syndrome in rat
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MIAO Yu-liang , DENG Xiao-ming, LI Jin-bao, ZHAO Xiang-kun, CAO Jin-mei， TIAN Ye-ping.
Department of Anaesthesiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
Objective: To investigate the effect of α -melanocyte stimulating hormone (α-MSH) on the apoptosis of the vascular endothelial cells of the lung in acute respiratory distress syndrome(ARDS) reproduced with acute hemorrhagic shock followed by intratracheal lipopolysaccharide(LPS,two-hit model) in rat.Methods: Ten male Sprague Dawley rats, weighing (33.7±2.5)g, were randomly divided into two groups(A and B) with 5 in each group. All rats were anesthesized and ventilated mechanically with fractional concentration of inspired oxygen (FiO 2) of 0.5, breath rate 100 times/min, tidal volume(V T) 12 ml/kg and inspiratory/expiratory ratio (I/E) 1∶15. The blood was withdrawn to induce hemorrhagic shock via the carotid artery until blood pressure reached (45±5)mm Hg(1 mm Hg=0.133 kPa), which was maintained for 1 hour, and the shed blood and Ringer's lactate in volume equal to the shed blood were reinfused in 2 hours for resuscitation. Afterwards, LPS was given via the tracheal (200 μg/kg，in 500 μl normal saline) to establish the ARDS model. Group A was ARDS control group, group B was α-MSH administration group. α-MSH was intravenously administrated simultaneously, 3 hours and 6 hours after LPS given, the dosage was 17 mg/kg at each time point. The rats were sacrificed at 9 hours after LPS challenge, and the lung tissue was examined with microscope and electron microscope to observe the pathological changes and apoptosis of the vascular endothelial cells. Results: In ARDS control group, remarkable infiltration of inflammatory cells was found in the alveoli, and the apoptosis of the vascular endothelial cells had developed to late stage. In α-MSH treatment group, few inflammatory cells were found in the alveoli, and the apoptosis of the endothelial cells was still in an early stage. Conclusion: α-MSH could inhibit the apoptosis of the vascular endothelial cells of the lung in the two-hit ARDS in rats. Therefore, it might have a protective effect on the lung after hemorrhagic shock and endotoxin challenge.
, 百拇医药
PAGE: 599-602
Value of blinded sampling methods in the pathogenic diagnosis of ventilator-associated pneumonia
CAI Shao-hua ， YU Sen-yang， ZHANG Jin-chuan， LIU Chang-ting， QIAN Gui-sheng.
Department of Geriatric-Respiratory, General Hospital of PLA, Beijing 100853, China
Objective: To evaluate the diagnostic value of blinded protected specimen brush and quantitative culture (BPSB-QC) in the pathogenic diagnosis of ventilator-associated pneumonia (VAP). Methods: A prospective, self-controlled clinical trial was conducted during a 36-month period. QC of paired samples of BPSB and PSB via a fiberoptic bronchoscopy(FOB- PSB) in a total of 54 patients during 125 suspected episodes of VAP was compared. The sensitivity and accuracy, as well as the concordance between BPSB-QC and FOB-PSB-QC result in 48 patients with 106 episodes of VAP were assessed. Both BPSB-QC and FOB-PSB-QC greater than 103 cfu/ml (positive cutoff) was considered diagnostic of VAP. Results: Forty-eight patients with 106 episodes were considered to have VAP (84.8%). The accuracy of BPSB-QC and FOB-PSB-QC were 80.8%(101/125) and 83.2%(104/125), respectively. The rate of complete concordant results was high (75.2%) for BPSB-QC and FOB-PSB-QC. The pathogenic diagnostic agreement between the two techniques was 84.8%(106/125). There were no significant differences with regard to site of pneumonia and positive diagnostic rate between the two techniques. Conclusion: BPSB-QC has similar accuracy and same feasibility compared with FOB-PSB-QC which was commonly primarily used in the pathogenic diagnosis of VAP, and that its use is substantially simpler, safe and cost saving, especially when FOB technique is not available.
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PAGE: 603
Rescue Acute Oranophosphones Pesticides Poisoning Muscles, Respiratory Parlysis Three Key Links
LUO Qing-yi, SUN Hong-wei
ICU Cao Country People's Hospital, Shandong 27440, China
PAGE:603-607
Effect of sustained inflation on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome in rabbit
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YANG Yi, QIU Hai-bo, YAN Yan-li, XU Hong-yang, WANG Li, ZHOU Shao-xia, ZHENG Rui-qiang.
Department of Critical Care Medicine, Zhong-da Hospital and School of Clinical Medicine, Southeast University, Nanjing 210009, Jiangsu, China
Objective: To evaluate the influence of sustained inflation (SI) on inflammatory response of extra pulmonary organs in acute respiratory distress syndrome(ARDS) in rabbit. Methods: The ARDS rabbit model were replicated by saline alveoli-lavage. The rabbits were randomly divided into four groups: ①normal group; ②ARDS group; ③low -volume(V T)+best end-expiratory pressure group(LVBP): V T 6 ml/kg, PEEP 2 cm H2O(1 cm H2O =0.098 kPa) greater than the pressure of lower inflection point in pressure-volume curve(Pinf); ④SI group: same settings as LVBP group plus SI performed every hour. After 4 hours of ventilation, liver and intestine homogenates were prepared to assay tumor necrosis factor(TNF)-α and interleukin(IL)-10 levels by enzyme-linked immunoadsorbent assay and their mRNA expression by reverse transcription-polymerase chain reaction. Myeloperoxidase (MPO) and malondialdehyde (MDA) were assessed at the same time. Results: In SI group, TNF-α mRNA expression in the liver and intestine tissues (24.82±7.88 and 25.06±10.57) were decreased significantly compared with ARDS(36.88±6.83 and 36.48±8.23) and LVBP groups (42.34±8.55 and 37.59±10.63, all P<0.05). The expression of IL-10 mRNA in the liver and intestine of rabbits in SI group(17.31±5.72 and 10.90±3.30) was lower than that of ARDS(24.64±3.85 and 24.31±7.69) and LVBP group(25.03±9.38 and 21.83±7.34, all P<0.05) signifantly. In SI group, the levels of TNF-α and IL-10 in liver and intestine tissue were similar to that of the other groups. The contents of MPO and MDA had no difference compared with the other groups too. Conclusion: SI can down-regulate inflammatory mediator expression of extra-pulmonary organs in rabbits with ARDS, and it may inhibit the inflammatory response in extra pulmonary organs.
, 百拇医药
PAGE:607
Clinical Efficacy on Na-DMPS Combined with Vecuronium in Emergency Treatment of Acute Tetramine Intoxication
Zhang Yong-tian, Tian Yong-qiang, Wang Wei-cheng, Kang Le-chang, Guo Chang-xing
Department of Emergency, Xiji People's Hospital, Ningxia 756200, China
PAGE: 608-610
Inflammatory injurious effect of angiotensin Ⅱ on pulmonary microvascular endothelium in rat
, 百拇医药
ZHANG Hong, SUN Geng-yun.
Department of Emergency, First Affiliated Hospital, Anhui Medical University, Hefei 230061, Anhui, China
Objective: To investigate the effects of angiotensin Ⅱ (AngⅡ) and its receptors on monolayer permeability of pulmonary microvascular endothelial cells in rat. Methods: The following examinations were done on cultured rat pulmonary microvascular endothelial cells (RPMVECs). ①Micro-infiltrator was used to assay the variations of lipopolysaccharide(LPS)-induced increased RPMVECs monolayer permeability coefficient (Kf) in different periods. ②Effect of angiotensin Ⅱ on LPS-induced permeability injury to the endothelium, and preventive effects of angiotensin Ⅱ type 1 receptor antagonist (Sar1, Ile8)-Ang Ⅱ. Results: LPS increased RPMVECs monolayer permeability compared with normal control, and AngⅡ exacerbated LPS-induced RPMVECs monolayer permeability significantly. This synergistic effect was significantly prevented by the addition of (Sar1, Ile8 )-Ang Ⅱ. Conclusion: AngⅡ and LPS have synergistic injurious effects to the pulmonary microvascular endothelium in rat, and AngⅡ exacerbates increase in LPS-induced RPMVECs monolayer permeability. This synergistic effect is significantly prevented by the addition of (Sar1, Ile8)-Ang Ⅱ.
, 百拇医药
PAGE:611-614
Protective effect of N-acetylcysteine on acute lung injury caused by exposure to rocket liquid propellant
LIU Song, YU Sen-yang. Department of Respiratory Diseases， General Hospital of PLA, Beijing 100853, China
Objective: To investigate the protective effect and its mechanisms of N-acetylcysteine (NAC) on acute lung injury (ALI) caused by exposure to high concentration rocket liquid propellants asymmetrical dimethylhydrazine (UDMH) and dinitrogen tetroxide (N 2O 4). Methods: Forty-two rats were randomly divided into three groups: the control group, the exposure group and the exposure plus the treatment group (NAC group). The rats of the latter two groups were exposed to UDMH 098 mg/L for 10 minutes and then N 2O 4 0.19 mg/L for another 10 minutes. After the exposure, the NAC group rats received immediately 150 mg/kg of NAC intravenously, and rein forced by intraperitoneal injection of NAC with a dose of 50 mg/kg 3 hours after the intravenous injection. The rats of other group were treated with saline in equal volume. All rats were killed after 6 hours. The lung wet to dry ratio (W/D ), the contents of lactate dehydrogenase (LDH) and total protein in bronchoalveolar lavage fluid (BALF), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in lung tissue, and the malondialdehyde (MDA) of plasma were measured. Pathological examination was performed. Results: The lung W/D ratio, the LDH and total protein in BALF, and the MDA of plasma were increased in the exposure group, while the activities of SOD and GSH-Px in lung tissue were decreased. The histopathology of the rats of exposure groups showed that there was exudation within alveolar spaces and prominant interstitial thickening of septa. In the NAC group, the values of the above findings were lowered, and the degree of lung injury was alleviated in histopathology. The lung W/D were negatively correlated with the activities of SOD and GSH-Px in lung tissue, and the correlation coefficient were-0662 (P<0.01) and -0707(P<0.01) respectively. Conclusion: The administration of NAC appears to attenuate the injury to the lung after an exposure to UDMH and N 2O 4 in high concentration, and the antioxidant activity of NAC may be responsible for the protective effect.
, 百拇医药
PAGE: 614
Management of acute abdomen caused by cholecystoenteral fistula, one case report of acute perforation of cholecystoduodenal fistula with carcinoma of rectum
LIU Chun, SHAN Dan
Department of General Surgery, Tianhe Hospital, Tianjin 300050, China
PAGE: 615-617
Clinical investigation of application of lung biopsy in the diagnosis of diffuse pulmonary interstitial disease
, 百拇医药
GAO Zhan-cheng, MA Mei-xing, GU Li-xin, LIU Jun, WANG Jun, SHEN Dan-hua.
Departments of Respiratory Medicine, Peking University, People's Hospital, Beijing
100044, China
Objective: To evaluate the application of lung biopsy in the diagnosis of diffuse pulmonary interstitial disease (DPID). Methods: The data of lung biopsy from 17 cases with uncertain DPID obtained during the period of July 1997 and December 2002 were retrospectively analysed. Results: All the cases were diagnosed by pathology. Three of them were diagnosed as sarcoidosis. Two were pulmonary alveolar proteinosis. One with usual interstitial pneumonia (UIP), one with bronchialitis obliterous and organizing pneumonia, one with nonspecific pneumonia, one with Castleman's disease, one with pulmonary lymph angioleiomyomatosis, two with bronchioalveolar carcinoma, one as rheumatoid pulmonary fibrosis complicated with pulmonary squamatous cancer and locally metastas is in lungs, two as pulmonary lymphangitic carcinomatosis, one as lung squamatous cancer with pulmonary lymphangitic metastasis, and one with primary pulmonary paraganglioma. Conclusion: Harvest of lung tissue directly by biopsy could be the most useful diagnostic method for DPID, and it is a highly valuable diagnostic tool for those patients whose diagnosis is uncertain with routine chest film and high resolution computed tomography.
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PAGE: 617
Application of the Technique of Continuous Blood Purification(CBP) to a case of Tetramine and Multiple Organ Failure(MOF) Complications
WU Xiao-ning, LIU Huan-hao
Nephro-intermal Medicine of the Fifth Affiliated Hospital of Guangxi Medical university, Liu zhou, Guangxi 545001, China
PAGE: 618-621
Study on effect of dexamethasone on the expression of matrix metalloproteinase and its tissue inhibitors in hyperoxia-induced lung injury
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LIU Cheng-jun, XU Feng, KUANG Feng-wu, DANG Run, LU Zhong-yi, WANG Xing-yong.
PICU of Children's Hospital of Chongqing University of Medical Sciences, Chongqing 400014, China
Objective: To observe the effect of dexamethasone on the mRNA expression of matrix metalloproteinase (MMPs) and tissue inhibitor of metalloproteinase(TIMPs) in the lung tissue and to explore the protective mechanism of dexamethasone in hyeroxia-induced lung injury. Methods: Thirty-two two-week old Wistar rats were randomly divided into atmospheric-air group (n=16) and hyperoxia group (n=16). After 7 days of continuous exposure to high concentration O 2(≥95%), the lung wet/dry(W/D) ratio, the protein content in bronchoalveolar lavage fluid(BALF) and histopathological changes of the lung were measured in 16 rats(8 in each group). The lung tissue specimens of the other 16 rats were cultured, 8 among which served as stmospheric-air control group, the remainder in the hyperoxia group were divided into hyperoxia control group,hyperoxia+dexamethasone (1×10-8mol/L) group, hyperoxia+dexamethasone (1×10-6 mol/L) group, and hyperoxia+dexamethasone (1×10-4 mol/L) group. Eight samples were examined in each group. After cultured for 24 hours, the lung tissue were collected and its mRNA expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: ①Pulmonary edema, hemorrhage and extensive inflammatory cells infiltration were observed in hyperoxia group, but no such changes were found in the atmospheric-air group. The lung W/D and the protein content in BALF in hyperoxia group were significantly higher than those in atmospheric air groups. ②The mRNA expressions of MMP-2, MMP-9, TIMP-1, TIMP-2 and the ratio of MMP-2/TIMP-2, MMP-9/TIMP-1 were significantly higher in the hyperoxic group than those in the atmospheric-air group. ③Dexamethasone could down-regulate the mRNA expressions of MMP-2 and MMP-9 in a concentration dependant manner. The mRNA expressions of TIMP-1, TIMP-2 also could be reduced by dexamethasone. Decreasing ratios of MMP-2/TIMP-2 and MMP-9/TIMP-1 were found in correlation with increasing concentration of dexamethasone. Conclusion:: Dexamethasone can reduce the mRNA expressions of MMPs as well as regulate the balance of MMPs/TIMPs, which may be one of the mechanism of its protective effect on hyperoxia-induced lung injury.
, 百拇医药
PAGE: 622-625
Change in Toll-like receptor 4 expression on interstitial macrophages in acute lung injury
ZHANG Wei, JIANG Yao-guang, XIE Zhi-jian, LU Fenglin, HU Cheng-xiang, LI Lei.
Department of Thoracic Surgery, South-West Hospital, Third Military Medical University, Chongqing 400038, China； Corresponding author: LI Lei. First Department, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
, 百拇医药
Objective: To observe changes in the level of Toll-like recepter 4(TLR4) mRNA and the expression of TLR4 protein in interstitial macrophages in serious thoracic injury. Methods: A rat model of severe thoracic trauma was reproduced,then interstitial macrophages were isolated and collected by enzymatic digestion before and after trauma cultured. TLR4 mRNA and the expression of TLR4 protein were measured by Northern and Western blotting before trauma and 2, 4, 8, 16, 24 hours after trauma.Results: A stable and reliable severe thoracic trauma model was successfully reproduced with 400 kPa impact on the up-right chest of rat by a multiple-function collision apparatus. The expression was up-regulated by trauma and the highest expression of interstitial macrophages occurred 8, 16 hours after the trauma, and returned to normal range at 24 hours. Conclusion: The up-regulation of TLR4 might participate in the pathological process of excessive inflammation after trauma to the lung.
, 百拇医药
PAGE:626-628
Study on molecular mechanisms of indirect suppression of albumin mRNA expression of hepatocytes by lipopolysaccharide in rat
WANG Xin-ying, LI Wei-qin, LI Ning, LI Jie-shou . Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, Jiangsu, China
Objective: To explore the molecular mechanisms of relationship between the decreased expression of albumin gene of the cultured hepatocytes by lipopolysaccharide (LPS) and cytokines. Methods: As an inhibitor of protein synthesis, cycloheximide (CHX) could inhibit the LPS-induced synthesis of cytokines. Therefore, the culture system pretreated with CHX could exclude the influence of cytokines on the regulation of albumin gene expression, then it might prove that LPS induced down-regulation of expression of albumin gene was a through cytokine-dependent pathway. The concentrations of interleukin(IL-6) and tumor necrosis factor-α(TNF -α) in the supernatant were also assayed. Results: The expression of albumin mRNA is inhibited significantly by LPS. CHX could block the pathway of LPS-induced down-regulation of expression of albumin gene at least at the transcription level. And the prevention was in a dose-related manner. The increase of IL-6 and TNF-α induced by LPS was inhibited by CHX(both P<0.05). Conclusion: The results suggest that LPS can indirectly inhibit the transcription of albuminogene through cytokines such as IL-6 and TNF-α.
, 百拇医药
PAGE: 629-631
Effects of norepinephrine on the therapeutic effect of nitric oxide inhalation in the treatment of acute respiratory distress syndrome in goat
LIU Shao-hua, XU Xin-rong, XU Ji, ZUO Xiang-rong, QIN Ping-ping.The First Affiliated Hospital of Nanjing Medicial University, Nanjing 210029, Jiangsu, China
Objective: To observe effects of norepinephrine (NE) on the therapeutic effects of nitric oxide (NO) inhalation in goats with endotoxin-induced acute respiratory distress syndrome (ARDS). Methods: A model of septic ARDS was reproduced by an intravenous infusion of low dose endotoxin in six goats, and then these animals were treated with 40×10-6 NO inhalation. After 30 minutes, intravenous infusion of NE in dose of 0.5 ug·kg-1·min-1 was given. The dynamic changes in gas exchange and hemodynamics were measured with the aid of Swan-Ganz catheter and arterial blood gas analysis before and after the onset of ARDS, 30 minutes after NO inhalation and administration of NE. Results: Inhalation of NO rapidly reduced mean pulmonary arterial pressure (MPAP), increased PaO2, decreased alveolar-arterial partial pressure of oxygen difference (P (A-a) O2) and intrapulmonary shunt fraction (Qs/Qt) in septic ARDS goats. These changes were more pronounced when NE was given compared with NO inhalaion alone. The combination of NO inhalation and NE infusion resulted in an increase in mean arterial pressure. Conclusion: Norepinephrine enhances the beneficial effect of nitric oxide inhalation on lung gas exchange in goats with endotoxin induced acute respiratory distress syndrome.
, 百拇医药
PAGE: 632
Applications of hemoperfusiion associated with hemodialysis in renal transplantation
ZHOU Shu-hua, GU Zhen-xiang, ZENG Sheng
Dezhou People‘s Hospital, Dezhou 253014, China
PAGE: 633
Clinic observations on pulmonary tidal volume during closed-chest lift-compression
WANG Li-xiang, WANG Fa-qiang, ZHANG Jian-peng, LIU Hui-liang, LEI Zhi-li, WEI Zhong-yu, XIA Hong-jie, LEI lianl-hui, WANG Yu-gui, ZHANG Jie, HOU Hui-ya
, 百拇医药
ICU, The General Hosptial of the Chinese people’s armed police forces, Beijing 100039, China
PAGE:634-635
The relationship between chlamydia Pneumoniae Infection and Asthma
GUO Qiang, ZHANG Lian-xiang, CAI Zhong
Tianjin Thoracic Hospital, Tianjin 300051, China
PAGE: 635
Hemo-purification in treatment of 6 cases with acute Dushugqiang poisoning
, 百拇医药
YU Qing-chun, WEI Jin-zhi, WEI Hong-zhen, SONG Ying-hua, WU Bin, WANG Yan-xu, Zang Yi-ming
Pingdingshan General Company of Coal Hospital, Henan 467000, China
PAGE: 636-638
The potential mechanisms underlying dissonance of cell-mediated immunity diring sepsis
DONG Yue-qing, YAO Yong-ming
Burns Institute, 304th Hospital of PLA, Beijing 100037, China
PAGE: 639-640
Local rennin-angiotensin system and sepsis
LI Jing-ling, YIN Chen-hong, ZHANG Shu-wen, WANG Bao-en
Department of infection and critical care medicine of (Beijing) friendship hospital affiliate of capital university of medical sciences, 百拇医药