针灸治疗大鼠溃疡性结肠炎细胞因子基因表达的探讨
结肠炎，溃疡性,治疗;针灸疗法;细胞因子;基因表达;RNA，信使,*国家自然科学基金资助项目，No.39670899.,项目负责人,*SupportedbytheNationalNaturalScienceFoundationofChina,No.39670899.,Correspondenceto,Geneexpression
上海市针灸经络研究所 200030吴焕淦，男，1956-11-21生，浙江省仙居县人，1990年浙江中医学院硕士，1993年上海中医药大学博士，副教授，副所长，主要从事针灸免疫学研究，发表论文20篇.
*国家自然科学基金资助项目，No.39670899.
项目负责人 吴焕淦，200030，上海市针灸经络研究所.
*Supported by the National Natural Science Foundation of China, No.39670899.
Correspondence to Vice-Prof. Huan-Gan Wu, Shanghai Institute of Acupuncture-Moxibustion and Meridians, 650 Wanping Nanlu, Shanghai 200030, China
Tel. +86·21·64395972
收稿日期 1998-07-07
Gene expression of cytokines in acupuncture and moxibustion treatment for ulcerative colitis in rats*
Huan-Gan Wu, Li-Bin Zhou, Cheng Huang, Ying-Ying Pan, Han Ping Chen, Zheng Shi and Xue-Gui Hua
Shanghai Institute of Acupuncture-Moxibustion and Meridians, Shanghai 200030, China
Abstract
AIM To observe the effect of acupuncture and moxibustion on the expression of IL-1β and IL-6 mRNA in ulcerative colitis rats.
METHODS Human fresh surgical colonic mucosa was homogenized by adding appropriate amount of normal saline and centrifuged at 3000r/min. Protein content of the supernatant was measured and then mixed with Freund adjuvant, and injected into the plantae of the model group rats, then into the plantea, dorsa, inguen and abdominal cavities (no Freund adjuvant for the last injection) on the 10th, 17th, 24th and 31st day respectively. When a certain titer of serum anti-colonic antibody was reached, 20mL/L formalin and antigen fluid (no Frenud adjuvant) were administered by enema to set up ulcerative colitis rat model. The animals were randomly divided into four groups: model control group (MC=8), electropuncture group (EP=8), herb spartition moxibustion group (HPM=8) and normal control group (NC=8). HPM: Mosa cones made of refined mugwort floss were placed on the medicinal pads (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupuncture once a day and 14 times in all. EP: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, all rats were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanatephenol-chloroform extraction method. RT-PCR technique was used to observe the expression of IL-1β and IL-6 mRNA. ......
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