螺杆菌感染;螺杆菌，幽门;细胞毒素相关蛋白基因A;基因表达;

幽门螺杆菌cagA基因的克隆表达及其产物的应用

http://www.100md.com 1998年12月15日《世界华人消化杂志》 1998年第12期

螺杆菌感染;螺杆菌，幽门;细胞毒素相关蛋白基因A;基因表达;聚合酶链反应,项目负责人,Correspondenceto,Hp,cagAgeneexpressionandapplicationofitsproduct,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings

     第四军医大学全军基因诊断技术应用研究所陕西省西安市 710033

    韩锋产，男，1963-05-08生，陕西省咸阳市人，汉族. 1985年延安大学医学系毕业，1990年西安医科大学生物化学硕士，1995年第四军医大学生物化学博士研究生，主要从事基因诊断方面的研究工作，发表论文10篇.

    项目负责人 韩锋产，710033，第四军医大学全军基因诊断技术应用研究所，陕西省西安市长乐西路17号.

    Correspondence to Feng-Chan Han, Chinese PLA Institute of Genetic Diagnosis, Fourth Military Medical University, 17 Changle Xilu, Xian 710033, Shaanxi Province, China

    Tel. +86·29·3285729

    E-mail address: Cuidx@igd.edu.cn

    收稿日期 1998-07-15

    Hp cagA gene expression and application of its product

    Feng-Chan Han, Xiao-Jun Yan and Cheng-Zhi Su

    Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xian 710033, Shaanxi Province, China

    

    Abstract

    AIM To express the cagA gene and develop a rapid immuno-detecting method for the infection of cagA+ Hp.

    METHODS We cloned the 5-end of cagA (854bp) into the expression vector pBV220 and transformed DH5α with the plasmid pBV220/fcagA, in which a single CagA fragment (FCagA) was produced when the temperature reached 42℃. After being renaturated, FcagA was purified by anion exchange and sephadex G-100 chromatography. The FcagA had a relative molecular weight of 38000. With the prepared FCagA, coloidal gold, and immunogold, we established the dot immunogold filtratoin assay (DIGFA) to detect anti-CagA antibody in serum. ......

    百拇医药网 http://www.100md.com/html/DirDu/1998/12/15/69/58/28.htm

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