HBsAgHBeAg作用PBLs对TCRVβ基因表达的影响
乙型肝炎病毒;肝肿瘤;乙型肝炎表面抗原;乙型肝炎e抗原;TCRVβ基因;基因表达,*国家自然科学基金资助项目，No.39770698和广东省五个一工程粤卫科[20号]资助.,,项目负责人,Correspondenceto,EffectsofHBsAgandHBeAgonTCRV,βgeneexpres
广东药学院分子生物学研究室 广东省广州市 510224黄树林，男，1953-03-15生，湖北省仙桃市人，汉族. 1980年湖北医科大学毕业，1987年湖北医科大学硕士，生物化学与分子生物学博士导师，主要从事肿瘤免疫调控与基因治疗的研究，发表论文43篇，出版专著1部.
*国家自然科学基金资助项目，No.39770698和广东省五个一工程粤卫科[20号]资助.
项目负责人 黄树林，510224，广东药学院分子生物学研究室，广东省广州市海珠区宝岗光汉直街40号.*
Correspondence to Shu-Lin Huang, Department of Molecular Biology, Guangdong Pharmacy College, 40 Baogang Guanghanzhijie, Haizhu District, Guangzhou 510224, Guangdong Province, China
Tel. +86·20·84429040 Ext. 440
收稿日期 1998-07-13 接收日期 1998-11-17
Effects of HBsAg and HBeAg on TCR V β gene expressions in peripheral blood lymphocytes*
Shu-Lin Huang, Hong-Qing Chen, Jing-Hua Li, Yi-Na Li and Li-QiongLuo
Department of Molecular Biology, Guangdong Pharmacy College, Guangzhou 510224, Guangdong Province, China
Abstract
AIM To investigate the relationship between hepatitis B virus (HBV) and hepatocellular carcinoma.
METHODS The TCR Vβ 1-20 gene repertoire was analysed by reverse transcription-polymerase chain reaction (RT-PCR) in peripheral blood lymphocytes (PBL) from individuals vaccinated with recombinant hepatitis B vaccine, PBLs from healthy donors stimulated by supernatants (HBsAg and HBeAg positive) of the HepG2 2215 cell line which was transfected by HBV-DNA. ......
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