Cyclin D1 amplification is associated with HBV DNA integration and pathology in human hepatocellular carcinoma*ª¤ª¤

    Hui-Yun Wang¹, Rui-Qi Yan¹, Jiang-Bin Long¹ and Qiu-Liang Wu<sup>2

    1</sup>Cancer Institute,ª¬²Department of Pathology, Cancer Center, Sun Yat-Sen University of Medical Sciences, Guangzhou 510060, Guangdong Province, Chinaª¤ª¤

    

    Abstractª¤

    AIM To explore the amplification of cyclin ª«D1ª« and its correlation to HBV infection and pathology during the development of human hepatocellular carcinoma (HCC).ª¤

    METHODS Fourty cases of fresh paired tumor and nontumor liver tissues from surgical resections were used for detection of genetic alterations of cyclin ª«D1ª«, CDK4 and HBV infection by the Southern hybridization. The samples were also examined histologically for pathological correlation with the genetic studies.ª¤

    RESULTS 2-to 30-fold amplifications of cyclin ª«D1ª« gene were detected in 9 of 39 HCC samples (23.7%) and a structural aberration of the gene was found in one of those samples with amplification. The amplifications of CDª«K4 gene were found in 5 of 37 HCC cases. HBV DNA was detected in 37 of 39 HCC samples (94.9%) and the integration of HBV DNA in 29 of 39 HCC samples (74.4%). By the correlation analysis, the cyclin ª«D1ª« amplification significantly correlated with the amplification of CDª«K4, the integrated HBV DNA, the differentiation degree and size of tumors. The correlation coefficients were 0.51, 0.31 ......