构建fas基因真核表达载体逆转胃癌耐药细胞MDR表型
fas基因;基因转导;多药耐药性;胃肿瘤,项目负责人,Correspondenceto,ConstructionofeukaryoticexpressionvectorpBK-fasandMDRreversaltestofdrug-resistantgastriccancercells,,Abstract,,AIM,METHODS,RESULTS,CONCLUS
第四军医大学西京医院消化疾病研究所 陕西省西安市 710033时永全，男，1973-11-03生，河南省上蔡县人，汉族. 1996年第四军医大学毕业，医学学士，现为西京医院消化内科硕士研究生，研究肿瘤相关基因的克隆与肿瘤基因治疗.
项目负责人 樊代明，710033，陕西省西安市，第四军医大学西京医院消化疾病研究所.
Correspondence to Dai-Ming Fan, Institute of Digestive Diseases, Xijing Hospital, 15 Changle West Road, Xi'an 710033,Shaanxi Province, China
Tel. +86·29·3375229
收稿日期 1998-11-26
Construction of eukaryotic expression vector pBK-fas and MDR reversal test of drug-resistant gastric cancer cells
Yong-Quan Shi, Bing Xiao, Ji-Yan Miao, Yan-Qiu Zhao, Han You and Dai-Ming Fan
Institute of Digestive Diseases, Xijing Hospital, 15 Changle West Road, Xi'an 710033, Shaanxi Province, China
Abstract
AIM To get fas overexpressing drug-resistant gastric cancer cells by gene transduction, to compare the expressing level of fas in gene transfected and non-transfected cells, and to observe drug sensitizing effect of fas gene in transfected cells.
METHODS With molecular cloning technique, the full length cDNA of fas was inserted into the multiple cloning site of the expressing vector pBK-CMV. Drug-resistant gastric cancer cell strain SGC7901/VCR was transpected with recombinant plasmids, and positive clones were selected by G418. Then the expressing levels of fas gene was determined by means of Northern blot and Western blot, and MTT assay was used to detect drug sensitivity of gene transfected cells. ......
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