胶体金免疫层析法检测抗幽门螺杆菌细胞毒素相关蛋白A抗体
螺杆菌,幽门;细胞毒素相关蛋白A,分析;胶体金;免疫层析;抗体,分析,项目负责人,Correspondenceto,Goldimmunochromatographicassayforanti-,Helicobacterpyloriantibody,Abstract,AIM,METHODS,RESULTS,CONCLUSI
中国人民解放军第四军医大学全军基因诊断技术研究所 陕西省西安市 710033韩锋产，男，1963-05-08生，陕西省咸阳市人，汉族. 1985年延安大学医学系毕业，1990年西安医科大学生物化学硕士，1995年第四军医大学生物化学博士，主要从事基因诊断方面的研究工作，发表论文12篇.
项目负责人 韩锋产，710033，陕西省西安市长乐西路17号，第四军医大学全军基因诊断技术研究所.
Correspondence to Feng-Chan Han, Chinese PLA Institute of Gene Diagnosis, Fourth Military Medical University, 17 Changle Xilu, Xi'an 710033, Shaanxi Province, China
Tel. +86·29·3374771
Email.Hanfc@263.net
收稿日期 1999-05-24 接收日期 1999-07-25
Gold immunochromatographic assay for anti-Helicobacter pylori antibody
Feng-Chan Han, Xiao-Jun Yan, Yu Hou, Le-Yi Xiao, Yan-Hai Guo and Cheng-Zhi Su
Chinese PLA Institute of Gene Diagnosis, Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China
Abstract
AIM To develop a rapid, simple and self-performing gold immunochromatographic assay (GICA) for the detection of anti-cytotoxin associated gene A(CagA) protein of Helicobacter pylori in sera.
METHODS With prepared GICA apparatus and colloidal gold which were coupled with protein A(SPA), the gold-SPA conjugate reacted with human IgG in serum and migrated along the nitrocellose strip on which the CagA was immobilized. If the serum contained anti-CagA IgG, there would be a red line on the strip where the CagA was immobilized. ......
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