胆囊结石患者胆汁、粘膜和结石需氧菌和厌氧菌脱氧核糖核酸研究
胆结石;DNA;细菌;胆汁,项目负责人,Correspondenceto,AstudyonDNAofaerobicandanaerobicbacteriainbile,mucosaandstoneingallstonepatients,,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings,摘要
中国人民解放军第一军医大学珠江医院肝胆外科 广东省广州市 510282方驰华,男,1958-06-20生,湖北省汉川市人,1981年毕业于第四军医大学附属空军医学院,1994年同济医科大学肝胆外科硕士,副主任医师. 主攻胆道外科尤其梗阻性黄疸基础、临床及手术、腹腔镜外科和重症急性胰腺炎. 发表学术论文265篇,参加编著2部,获全军科技奖15项.
项目负责人 方驰华,510282,广东省广州市工业大道中253号,中国人民解放军第一军医大学珠江医院肝胆外科.
Correspondence to Chi-Hua Fang, Department of Hepatobiliary Surgery, Zhujiang Hospital, 253 Gongye Avenue, Guangzhou 510282, Guangdong Province, China
Tel. +86-20-85143557
收稿日期 1999-06-30 接收日期 1999-11-20
A study on DNA of aerobic and anaerobic bacteria in bile, mucosa and stone in gallstone patients
Chi-Hua Fang
Department of Hepatobiliary Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China
Abstract
AIM To study bacteria deoxyribonucleic acid (DNA) in bile, mucosa and stone, and provide evidence of molecular biology of bacteria in gallstones formation.
METHODS DNA of five aerobic bacteria including Bacillus Coli, Kelbsiella Pneumonie, Bacillus Typhoid, Helicobacter pylori, Bacillus Proteus and four anaerobic bacteria including Bacteroides Fragilis, Peptococcus, Clostridium and Bacillus Perfringens in previous samples was tested with polymerase chain reaction (PCR) in 70 gallstone patients. ......
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