正反义人端粒酶RNA组分(hTR)基因真核表达载体的构建
人端粒酶RNA组分;端粒酶;真核表达载体;反义基因,国家自然科学基金资助项目，,No.39770302,项目负责人,SupportedbytheNationalNaturalScienceFoundationofChina,No.39770302,,Correspondenceto,Constructionofsensea
中国人民解放军第三军医大学西南医院消化内科 重庆市 400038杨金亮，男，1967-08-13生，山西省平顺人，汉族. 1991年第三军医大学预防医学专业毕业，学士，1997年第三军医大学内科学硕士，主治医师，在读博士研究生(导师专家房殿春)，主要从事胃癌的免疫和基因治疗研究，发表论文8篇.
国家自然科学基金资助项目，No.39770302
项目负责人 杨金亮，400038，重庆市第三军医大学西南医院消化内科.
Department of Gastroenterology of Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Supported by the National Natural Science Foundation of China, No.39770302
Correspondence to Jin-Liang Yang, Department of Gastroenterology, Southwest Hospital, Third Military Medical University,Chongqing 400038, China
Tel. 0086-23-65328301 Ext.73094
Email. yyjjll@usa.net
收稿日凇999-12-22 接收日期 2000-01-28
Construction of sense and antisense hTR eukaryotic expression vector
Jin-Liang Yang, Dian-Chun Fang, Shi-Ming Yang,Yuan-Hui Luo, Rong Lu, Kun-Lun Luo and Wei-Wen Liu
Abstract
AIM To construct an eukaryotic expression vector for sense and antisense human telomerase RNA components (hTR) gene.
METHODS The hTR cDNA fragment contained in the pGRN83 vector from restriction endonuclease digestion was inserted into eukaryotic expression vector pcl-neo in cis-direction or trans-direction using DNA recombinant technology. ......
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