散发性大肠癌组织及粪便P53蛋白、K-ras及APC基因的检测
结直肠肿瘤,病理学;诊断;粪便,细胞学;蛋白质P53,分析;基因,ras;基因,APC,项目负责人,DetectionofP53protein,K-rasandAPCgenemutationinsporadiccolorectalcancertissueandexfoliativeepithelialcellsinstool,Correspondence
中国人民解放军北京军区总医院消化内科 北京市 100700范如英, 女, 1964-05-12生，河南省新乡市人，汉族. 1984年新乡医学院医疗系毕业，1998年兰州医学院获消化内科硕士学位，主要从事大肠癌诊治研究，发表论文13篇.
项目负责人 范如英，100700，北京市，中国人民解放军北京军区总医院消化内科，现系第三军医大学消化内科专业在读博士生.
Detection of P53 protein, K-ras and APC gene mutation in sporadic colorectal cancer tissue and exfoliative epithelial cells in stool
Ru Ying Fan, Shi Rong Li, Zi Tao Wu and Xia Wu
Department of Gastroenterology, General Hospital of Beijing Army Region, Beijing 100700, China
Correspondence to: Ru Ying Fan, Department of Gastroenterology, General Hospital of Beijing Army Region, Beijing 100700, China
Received 2001-03-19 Accepted 2001-04-08
Abstract
AIM To investigate the mutation of P53, K-ras and APC gene in human stools and its significance in diagnosis of colorectal cancer.
METHODS Tumor tissue and colorectal exfoliative cells in stools were collected from 27 patients and then, using S-P method, P53 protein was detected. The mutation of K-ras code 12, 13 and APC gene at exon 15 MCR in 22 specimens of tumor mucosa, 9 specimens of normal mucosa and 10 samples of stool were determined by polymerase chain reaction amplification and restriction enzy me analysis (PCR-RFLP) and polymerase chain reaction and its single strand conformation polymorphism (PCR-SSCP) respectively. ......
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