人肝再生增强因子在毕赤酵母中的表达
人肝再生增强因子;肝再生;肝细胞生长因子,遗传学;毕赤酵母,遗传学;基因表达;遗传载体摘要,项目负责人,ExpressionofhumanaugmenterofliverregenerationinPichiapastoris,Correspondenceto:,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjec
中国人民解放军302医院基因治疗研究中心 北京市 100039夏小兵, 男, 1971-03-11生, 江西省永新县人, 汉族. 1992年武汉大学毕业, 学士学位, 1995年与1999年分别获得军事医学科学院医学硕士与理学博士学位. 主要从事生物工程研究.
项目负责人 成军, 100039, 北京丰台路26号, 中国人民解放军302医院基因治疗研究中心.
Expression of human augmenter of liver regeneration in Pichia pastoris
Xiao Bing Xia, Jun Cheng, Gang Wang, Ji Zhen Yang, Yan Liu, Jing Dong, Lin Wang and Ke Li
Genetherapy Research Center, the 302nd Hospital of PLA, China
Correspondence to:Dr. Xiao Bing Xia, Gene-therapy Research Center, the 302 nd Hospital of PLA, 26 Fengtai Road,Beijing 100039, China
Tel. 0086-10-66933392, Fax. 0086-10-63801283
Email. xb@genetherapy.com.cn
Received 2001-02-06 Accepted 2001-02-12
Abstract
AIM To clone the human augmenter of liver regeneration (hALR) cDNA from the human fetal liver cDNA panel, and to express hALR in Pichia pastoris.
METHODS hALR cDNA was amplified by PCR, and then was sequenced. The expression vector was constructed after in subcloning hALR cDNA into the downstream of the α-factor secretion signal under the control of AOX1 promoter of the pPIC9K vector. His+ recombinant clones that can resist 4.0g·L-1 G418 and were selected, and hALR cDNA insertion was analyzed by PCR. The expression of recombinant Pichia pastoris strains were conducted under the inducer of methanol and analyzed by SDS-PAGE. ......
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