福建医科大学附属协和医院消化内科 福建省福州市 350001

    李丹，女，1976-11-22生，福建省福清市人，汉族，1999年福建医科大学临床医疗系毕业.同年考取福建医科大学硕士研究生，主要从事肝脏疾病研究.

    福建省教委基金资助项目. No. JA 01070

    项目负责人 王小众, 350001, 福州市新权路29号 福建医科大学附 属协和医院消化内科. drwangxz@public6.fz.fj.cn

    Telephone: 0591-3357896 Ext 8482

    收稿日期 2001-07-05 接受日期 2001-07-11

    Effects of TNFα, IL-6 and IL-10 on the development of experimental r at liver fibrosis

    Dan Li, Li-Juan Zhang, Zhi-Xin Chen, Yue-Hong Huang and Xiao-Zhong Wang

    Department of Gastroenterology, Union Hospital, Fu Jian Medical Univ ersity, Fu zhou 350001, Fu jian Province, China

    Supported by the Fund of Education Commision, Fu jian Province, China No . JA 01070

    Correspondence to: Dr. Xiao-Zhong Wang, Department of Gastroent erology, Union Hospital, Fu Jian Medical University, Fu zhou 350001, Fu jian Pro vince, China

    Received 2001-07-05 Accepted 2001-07-11

    Abstract

    AIM To investigate the role of tumor necrosis factorα, inte rleukin-6 and interleukin-10 on experimental rat liver fibrosis.

    METHODS One hundred clean SD rats were divided randomly into 3 groups: control group (intraperitoneal injection of saline 2mL·kg^-1,2· wk^-1), model group(intraperitoneal injection of 500mL·L^-1CCl₄ 2 mL·kg^-1,2·wk^-1), IL-10 group (in addition to the same dosage o f CCl₄, intraperitoneal injection of IL-10 4μg·kg^-1 was made from week 3). In week 5, 7 and 9, the rats of the three groups were sel ected randomly to collect plasma and resect liver. The serum levels of TNF-α, IL-6 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA), li ver tissues were made into paraffin section with sliver staining. ......