陆荫英，李克，成军，王琳，刘妍，段惠娟，张玲霞，中国人民解放军第302医院传染病研究所基因治疗研究中心 北京市 100039

    陆荫英，1974-08-27，女，贵州省贵阳人，2000年军医进修学院传染病学专业博士研究生，发表论文3篇.

    项目负责人 成军，100039，北京市，丰台区丰台路26号基因治疗研究中心. cj@genetherapy.com.cn

    电话: 010-66933392

    收稿日期 2001-10-21 接受日期 2001-11-01

    Cloning and expression of hepatitis B virus X gene in yeast

    Yin-Ying Lu, Ke Li, Jun Cheng, Lin Wang, Yan Liu, Hui-juan Duan，Ling-Xia Zhang

    Yin-Ying Lu, Ke Li, Jun Cheng, Lin Wang, Yan Liu, Hui-juan Duan，Ling-Xia Zhang.Gene Therapy Research Center, Institution of Infectious Diseases, Chinese PLA 302 Hospital, 100039, Beijing, China

    Correspondence to: Yin-Ying Lu, Gene Therapy Research Center, Institution of Infectious Diseases, PLA 302 Hospital, Luyinying 100039, Beijing, China. luyinying@263.net

    Received 2001-10-21 Accepted 2001-11-01

    

    AbstractAIM:To study the exact function of HBx protein so as to investigate the gene expression of HBxAg in yeast.

    METHODS:PCR was used to amplify the gene of HBx from the plasmid pCP10 containing the whole fragment of ayw subtype of HBV and the PCR product was cloned into pGEM-T vector and was evaluated by sequencing. The gene of HBx was cut from pGEM-T vector and cloned into yeast expression plasmid pGBKT7, and was transformed into yeast cell AH109. The yeast protein was isolated, and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. ......