潘勤，李定国，汪余勤，徐芹芳，上海第二医科大学附属新华医院消化内科 上海市200092

    潘勤，男，1971-03-12生，上海市人，汉族.1994年上海铁道医学院本科毕业，2000年上海第二医科大学硕士研究生毕业，现为上海第二医科大学博士研究生.主要从事肝纤维化的发生机制及药物防治研究.

    项目负责人 潘勤，200092，上海市控江路1665号，上海第二医科大学附属新华医院. fangchunhua@online.sh.cn

    电话:021-65790000-5319 传真:021-55296348

    收稿日期 2002-05-02 接受日期 2002-06-10

    Effects of somatostatin on activated rat hepatic stellate cells and its mechanisms

    Qin Pan, Ding-Guo Li, Yue-Qin Wang, Qin-Fang Xue

    Qin Pan, Ding-Guo Li, Yue-Qin Wang, Qin-Fang Xue, Department of Gastroenterology, Xin Hua Hospital, Shanghai Second Medical University, Shanghai 200092, China

    Correspondence to: Qin Pan, Xin Hua Hospital, 1665 KongJiang Road, Shanghai 200092, China. fangchunhua@online.sh.cn

    Received 2002-05-02 Accepted 2002-06-10

    AbstractAIM:To investigate the effects of somatostatin on proliferation and apoptosis of activated rat hepatic stellate cells (HSCs).

    METHODS:Being isolated from liver of SD rat by in situ perfusion with collagenase and pronase and density gradient, HSCs became activated after 10 days. Then the rates of apoptosis and proliferation of HSCs, which had been treated with different dosage of somatostatin, were measured by acridine orange/ethidium bromide fluorescent stain, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, transmission electron microscopy, flow cytometry and MTT assay. Furthermore, the mechanisms of somatostatin were investigated with the aid of cytodynamic analysis. ......