烧伤湿性医疗技术对表皮再生干细胞作用的研究

http://www.100md.com 《中国烧伤创疡杂志》 2000年第3期

中国烧伤创疡科技中心(100053)徐荣祥许增禄,[摘要]目的：,方法：,结果：,结论：,[关键词],TheEffectofMEBTonEpidermalRegenerativeStemCells,[Abstract

     中国烧伤创疡科技中心(100053) 徐荣祥许增禄

    [摘要] 目的：探索烧伤湿性医疗技术于浅Ⅲ度烧伤时脂肪层烧伤创面自然修复的机制。方法：应用细胞免疫化学方法生物素抗生物素蛋白(biotinavidi n)DCS体系间接免疫荧光技术。以小鼠抗人角蛋白19的单克隆抗体检测表皮再生干细胞。结果：烧伤后24h可见表皮再生干细胞出现。烧伤后4天表皮再生干细胞数量增多。烧伤后7天和14天表皮再生干细胞数量最多。烧伤后21天和28天表皮再生干细胞减少。结论：烧伤湿性医疗技术对浅Ⅲ度烧伤残余组织中的再生表皮干细胞具有促进其激活和增生的作用。

    [关键词] 烧伤湿性医疗技术表皮干细胞细胞免疫化学

    The Effect of MEBT on Epidermal Regenerative Stem Cells Xu Ronxiang, Xu Zenglu, China National Science and Technology Center for Burns, Wounds and Ulce rs, Beijing,100053

    [Abstract] Objective:To investigate the mechanism of the effect of M EBT on the s pontaneous repair of fatty layer burn wound of superficial third degree burn. Me thod: Immunocytochemistry method---biotin-avidin DCS system, indirect immunofluo rescence technique (IFT) was applied. Mouse antihuman type 19 keratin monoclona l antibody (McAb) was used to detect epidermal regenerative stem cells. Results: Epidermal regenerative stem cells emerged in 24 hr post burn and the number of epidermal regenerative stem cell increased on day 4 post burn. On days 7 and 14 post burn, the number of epidermal stem cells increased to the peak. On days 21 and 28 post burn, the number of epidermal stem cells decreased. Conclusion: MEBT has the effect of promoting the activation and proliferation o f regenerative epidermal stem cell in the residual surviving tissue of superfici al third degree burn wounds. ......

百拇医药网 http://www.100md.com/html/DirDu/2004/11/05/92/38/31.htm

您现在查看是摘要页，全文长 5957 字符。