内源性一氧化氮对急性坏死性胰腺炎大鼠肠黏膜通透性的影响
杨永久,辽宁朝阳市中心医院普通外科 辽宁省朝阳市 122000
高乃荣,东南大学附属中大医院普通外科 江苏省南京市 210009
项目负责人:杨永久, 122000, 辽宁省朝阳市朝阳大街二段6号, 朝阳中心医院. doctoryyj@sohu.com
电话: 0421-2816477
收稿日期: 2004-11-09 接受日期: 2004-12-08
摘要
目的:探讨内源性一氧化氮(NO)对急性坏死性胰腺炎(ANP)大鼠肠黏膜通透性的影响.
方法:84只Sprague-Dawley大鼠随机分为4组:假手术组，生理盐水组(ANP/NS)，硫酸甲基异硫脲组(ANP/SMT)，精氨酸组(ANP/L-Arg).测定12 h和24 h 血浆和小肠黏膜组织NO含量，观察小肠的病理改变，肠黏膜通透性以及脏器细菌移位率.
结果:ANP大鼠病程中存在NO含量降低、肠黏膜损伤，出现肠黏膜通透性升高和细菌移位，应用SMT可使NO的含量更加减少，肠黏膜损伤更加严重，肠黏膜通透性和脏器细菌移位率显著增加;应用左旋精氨酸(L-Arg)后小肠的病理损害明显减轻，肠黏膜的通透性显著降低，脏器细菌移位率显著减少.
结论:ANP大鼠病程中存在内源性NO水平降低，肠黏膜通透性升高，适当调节内源性NO的水平对降低ANP肠黏膜通透性具有一定的保护作用.
杨永久, 高乃荣. 内源性一氧化氮对急性坏死性胰腺炎大鼠肠黏膜通透性的影响. 世界华人消化杂志 2005;13(3):389-391
Aho HJ,Koskensalo ML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodiumtaurocholate-induced
acute haemorrhagic pancreatitis. Scand J Gastroenterol 1980;15:411-416
2 Resnick RH,Royal H, Marshall W, Barron R, Werth T. Intestinal permeability ingastrointestinal disorders, Use of
oral [99mTc]DTPA.Dig Dis Sci 1990;35:205-211
3 Ammori BJ, Leeder PC, King RF, Barclay GR, Martin IG, Larvin M, McMahon MJ. Early increase in intestinal permeability
in patients with severe acute pancreatitis:correlation with endotoxemia, organ failure, and mortality.
J Gastrointest Surg 1999;3:252-262
4 Hotz HG, Foitzik T, Roheder J, Schulzke JD, Fromm M, Runkel NS, Buhr HJ. Intestinal microcirculation and gut
permeability in acute pancreatitis:early changes and therapeutic implications. J Gastrointest Surg 1998;2:518-525
5 Kurose I, Wolf R, Grisham MB, Granger DN. Modulation of ischemia -reperfusion induced microvascular dysfunction by
nitric oxide. Circ Res 1994;74:376-382
6 Dobosz M, Hac S, Wajda Z. Dose nitric oxide protect from microcirculatory disturbances in experimental acute
pancreatitis in rat? Int J Microcirc Clin Exp 1996;16:221-226
编辑 张海宁, 百拇医药
高乃荣,东南大学附属中大医院普通外科 江苏省南京市 210009
项目负责人:杨永久, 122000, 辽宁省朝阳市朝阳大街二段6号, 朝阳中心医院. doctoryyj@sohu.com
电话: 0421-2816477
收稿日期: 2004-11-09 接受日期: 2004-12-08
摘要
目的:探讨内源性一氧化氮(NO)对急性坏死性胰腺炎(ANP)大鼠肠黏膜通透性的影响.
方法:84只Sprague-Dawley大鼠随机分为4组:假手术组，生理盐水组(ANP/NS)，硫酸甲基异硫脲组(ANP/SMT)，精氨酸组(ANP/L-Arg).测定12 h和24 h 血浆和小肠黏膜组织NO含量，观察小肠的病理改变，肠黏膜通透性以及脏器细菌移位率.
结果:ANP大鼠病程中存在NO含量降低、肠黏膜损伤，出现肠黏膜通透性升高和细菌移位，应用SMT可使NO的含量更加减少，肠黏膜损伤更加严重，肠黏膜通透性和脏器细菌移位率显著增加;应用左旋精氨酸(L-Arg)后小肠的病理损害明显减轻，肠黏膜的通透性显著降低，脏器细菌移位率显著减少.
结论:ANP大鼠病程中存在内源性NO水平降低，肠黏膜通透性升高，适当调节内源性NO的水平对降低ANP肠黏膜通透性具有一定的保护作用.
杨永久, 高乃荣. 内源性一氧化氮对急性坏死性胰腺炎大鼠肠黏膜通透性的影响. 世界华人消化杂志 2005;13(3):389-391
Aho HJ,Koskensalo ML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodiumtaurocholate-induced
acute haemorrhagic pancreatitis. Scand J Gastroenterol 1980;15:411-416
2 Resnick RH,Royal H, Marshall W, Barron R, Werth T. Intestinal permeability ingastrointestinal disorders, Use of
oral [99mTc]DTPA.Dig Dis Sci 1990;35:205-211
3 Ammori BJ, Leeder PC, King RF, Barclay GR, Martin IG, Larvin M, McMahon MJ. Early increase in intestinal permeability
in patients with severe acute pancreatitis:correlation with endotoxemia, organ failure, and mortality.
J Gastrointest Surg 1999;3:252-262
4 Hotz HG, Foitzik T, Roheder J, Schulzke JD, Fromm M, Runkel NS, Buhr HJ. Intestinal microcirculation and gut
permeability in acute pancreatitis:early changes and therapeutic implications. J Gastrointest Surg 1998;2:518-525
5 Kurose I, Wolf R, Grisham MB, Granger DN. Modulation of ischemia -reperfusion induced microvascular dysfunction by
nitric oxide. Circ Res 1994;74:376-382
6 Dobosz M, Hac S, Wajda Z. Dose nitric oxide protect from microcirculatory disturbances in experimental acute
pancreatitis in rat? Int J Microcirc Clin Exp 1996;16:221-226
编辑 张海宁, 百拇医药
