枳实消痞颗粒对糖尿病大鼠血浆和胃组织中胃肠激素的影响

http://www.100md.com 2005年3月15日 《世界华人消化杂志》 2005年第6期

     黄妙珍,杭州市第二人民医院消化科 浙江省杭州市 310015

    杭州市卫生局资助项目, No. 2002B013

    通讯作者:黄妙珍, 310015, 浙江省杭州市温州路126号, 杭州市第二人民医院. hmzhope@sina.com

    电话: 0571-88303653

    收稿日期: 2005-01-25 接受日期: 2005-02-26

    摘要

    目的:探讨枳实消痞颗粒对糖尿病大鼠胃肠激素的影响.

    方法:SD雄性大鼠60只，随机选9只为正常组，不造模，其余大鼠禁食不禁水16 h，用四氧嘧啶进行腹腔注射，72 h后大鼠血糖大于11.1 mmol/L的为糖尿病模型成立.糖尿病大鼠随机分枳实消痞颗粒治疗组、多潘力酮混悬液对照组、模型组，各17只.每日相应灌枳实消痞颗粒溶液、多潘力酮混悬液和蒸馏水.实验4 wk后测定各大鼠血浆和胃组织中胃泌素、胃动素、胰高糖素、P物质.

    结果:模型组、治疗组大鼠血浆胃泌素水平均低于正常组大鼠(t= 3.03，P= 0.01;t = 2.29，P= 0.04).模型组大鼠血浆胃动素水平低于正常组大鼠(t= 2.17，P= 0.04).治疗组、对照组大鼠胃动素水平均上升(t= 2.26，P= 0.04;t = 2.66，P= 0.02).模型组、对照组大鼠胰高糖素水平均高于正常组(t= 2.41，P= 0.03;t = 2.61，P= 0.02).P物质在各大鼠中表达无显著差异.模型组大鼠胃组织中胃泌素、胃动素水平比正常偏低，而枳实消痞颗粒能显著提高他们的水平(t= 2.43，P = 0.03;t = 2.25，P = 0.04).与模型组相比，对照组胃动素水平也显著上升(t= 2.22，P = 0.04).在大鼠胃组织中没有测出胰高糖素和P物质.

    结论:枳实消痞颗粒能通过提高糖尿病大鼠血浆胃动素水平、胃组织中胃泌素和胃动素水平而改善其胃肠功能.

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