枳实消痞颗粒对糖尿病大鼠血浆和胃组织中胃肠激素的影响
黄妙珍,杭州市第二人民医院消化科 浙江省杭州市 310015
杭州市卫生局资助项目, No. 2002B013
通讯作者:黄妙珍, 310015, 浙江省杭州市温州路126号, 杭州市第二人民医院. hmzhope@sina.com
电话: 0571-88303653
收稿日期: 2005-01-25 接受日期: 2005-02-26
摘要
目的:探讨枳实消痞颗粒对糖尿病大鼠胃肠激素的影响.
, http://www.100md.com
方法:SD雄性大鼠60只，随机选9只为正常组，不造模，其余大鼠禁食不禁水16 h，用四氧嘧啶进行腹腔注射，72 h后大鼠血糖大于11.1 mmol/L的为糖尿病模型成立.糖尿病大鼠随机分枳实消痞颗粒治疗组、多潘力酮混悬液对照组、模型组，各17只.每日相应灌枳实消痞颗粒溶液、多潘力酮混悬液和蒸馏水.实验4 wk后测定各大鼠血浆和胃组织中胃泌素、胃动素、胰高糖素、P物质.
结果:模型组、治疗组大鼠血浆胃泌素水平均低于正常组大鼠(t= 3.03，P= 0.01;t = 2.29，P= 0.04).模型组大鼠血浆胃动素水平低于正常组大鼠(t= 2.17，P= 0.04).治疗组、对照组大鼠胃动素水平均上升(t= 2.26，P= 0.04;t = 2.66，P= 0.02).模型组、对照组大鼠胰高糖素水平均高于正常组(t= 2.41，P= 0.03;t = 2.61，P= 0.02).P物质在各大鼠中表达无显著差异.模型组大鼠胃组织中胃泌素、胃动素水平比正常偏低，而枳实消痞颗粒能显著提高他们的水平(t= 2.43，P = 0.03;t = 2.25，P = 0.04).与模型组相比，对照组胃动素水平也显著上升(t= 2.22，P = 0.04).在大鼠胃组织中没有测出胰高糖素和P物质.
, 百拇医药
结论:枳实消痞颗粒能通过提高糖尿病大鼠血浆胃动素水平、胃组织中胃泌素和胃动素水平而改善其胃肠功能.
黄妙珍. 枳实消痞颗粒对糖尿病大鼠血浆和胃组织中胃肠激素的影响. 世界华人消化杂志 2005;13(6):790-7931 Domschke S, Bloom SR, Adrian TE, Lux G, Domschke W. Chronic pancreatitis and diabetes mellitus:plasma and
gastroduodenal mucosal profiles of regulatory peptides(gastrin, motilin, secretin, cholecystokinin, gastric inhibitory
polypeptide, somatostatin, VIP, substance P, pancreatic polypeptide, glucagon, enteroglucagon, neurotensin).
, http://www.100md.com
Hepatogastroenterology 1988;35:229-237
2 Pohle T, Domschke W. Gastric function measurements in drug development. Br J Clin Pharmacol 2003;56:156-164
3 Inui H, Yasuno R, Takenoshita M, Ohnishi Y, Sakamoto M, Matsuzaki J, Yamaji R, Miyatake K, Yamatodani A, Nakano Y.
Increases in gastric histidine decarboxylase activity and plasma gastrin level in streptozotocin-induced type 1 diabetic
, 百拇医药
rats. J Nutr Sci Vitaminol(Tokyo) 2000;46:144-148
4 Migdalis L, Thomaides T, Chairopoulos C, Kalogeropoulou C, Charalabides J, Mantzara F. Changes of gastric emptying
rate and gastrin levels are early indicators of autonomic neuropathy in type II diabetic patients. Clin Auton Res
2001;11:259-263
5 Rong SL, Li TG, Fang HM, Zheng YP. Effect of Chinese herbs for cool-moistening and freeing collaterals on serum
, http://www.100md.com
gastrin and surface electrogastrogram in patients of diabetes mellitus with gastroparesis.
Zhongguo Zhongxiyi Jiehe Zazhi 2004;24:976-978
6 El-Salhy M. Gastrointestinal transit in an animal model of human diabetes type 2: relationship to gut neuroendocrine
peptide contents. Ups J Med Sci 2002;107:101-110
7 Rooman I, Bouwens L. Combined gastrin and epidermal growth factor treatment induces islet regeneration and restores
, http://www.100md.com
normoglycaemia in C57Bl6/J mice treated with alloxan. Diabetologia 2004;47:259-265
8 Xu S, Yang Q, Cheng XF, Zhu JH, Xu JL. Effect of Lewei Fruid retention on gastrin and motilin in experimental model
of Spleen-deficiency rats. Zhongguo Yiyao Xuebao 2004;19:20-22
9 Xiao W, Liu J, Liu LY. Effect of jianpi wenshen decoction on serum gastrin, plasma motilin and somatostatin in patients
, http://www.100md.com
of diabetic diarrhea. Zhongguo Zhongxiyi Jiehe Zazhi 2002;22:587-589
10 Nilsson BI, Svenberg T, Tollstrom T, Hellstrom PM, Samuelson K, Schnell PO. Relationship between interdigestive
gallbladder emptying, plasma motilin and migrating motor complex in man. Acta Physiol Scand 1990;139:55-61
11 Zhou L, Wang LJ, Yuan B, Wang L. Effect of motilin on gastric smooth contraction induced by interstitial cells of
, http://www.100md.com
Cajal. Zhonghua Yixue Zazhi 2003;83:1422-1427
12 Huang J, Zhou H, Mahavadi S, Sriwai W, Lyall V, Murthy KS. Signaling pathways mediating gastrointestinal smooth
muscle contraction and MLC20 phosphorylation by motilin receptors. Am J Physiol Gastrointest Liver Physiol
2005;288:G23-31
13 Li H, Li GC. Changes of plasma MOT and antroduodenal SP expression in rats with functional dyspepsia and effect
, 百拇医药
of Qingzhishu. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:12-15
14 Liu SL, Mei GQ, Zhao YQ, Sun JQ, Wan XG, Zhang ML. Effect of Shugan Hewei decoction on motilin and gastrin in
blood of functional dyspepsia rats. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2004;12:198-200
15 Bai CC, Ma C, Li JY. Effect of Hewei decotion in motilin and gastrin with functional dyspepsia.
, http://www.100md.com
Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:373-374
16 Mao BY, Huang GF. Study of Jianpi Liqi medicine on gastrointestinal function and plasma motilin with dyspepsia.
Xin Zhongyi 2003;35:31-33
17 Xu SJ, Tang CH, Li XL, Dai Y. Effect of Yiqi Zengye granule on serum gastrin and plasma motilin in experimental model
of Spleen-deficiency diarrhea. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:9-11
, http://www.100md.com
18 Guo YQ, Yao SK, Fan HY, Tian YX, Huang SY, Feng L. Effect of Qingre Huashi detoction on serum gastrin, motilin,TNF-a1 and IL-10 in the dyspepsia patients with spleen-stomach damp-heat syndrome. Zhongguo Zhongyi Jichu
Yixue Zazhi 2004;10:63
19 Yuan J, Liu SY. Fasting serum motilin level and clinical meanings in diabetes. Dongnan Daxue Xuebao(Yixueban)
2003;22:262263
, 百拇医药
20 El-Salhy M, Spangeus A. Gastric emptying in animal models of human diabetes:correlation to blood glucose level and
gut neuroendocrine peptide content. Ups J Med Sci 2002;107:89-99
21 Kawagishi T, Nishizawa Y, Okuno Y, Sekiya K, Morii H. Effect of cisapride on gastric emptying of indigestible solids
and plasma motilin concentration in diabetic autonomic neuropathy. Am J Gastroenterol 1993;88:933-938
, 百拇医药
22 Peeters TL, Muls E, Janssens J, Urbain JL, Bex M, Van Cutsem E, Depoortere I, De Roo M, Vantrappen G, Bouillon R.
Effect of motilin on gastric emptying in patients with diabetic gastroparesis. Gastroenterology 1992;102:97-101
23 Vazeou A, Papadopoulou A, Papadimitriou A, Kitsou E, Stathatos M, Bartsocas CS. Autonomic neuropathy and
gastrointestinal motility disorders in children and adolescents with type 1 diabetes mellitus. J Pediatr Gastroenterol
, 百拇医药
Nutr 2004;38:61-65
24 Greenbaum CJ, Prigeon RL, D'Alessio DA. Impaired beta-cell function, incretin effect, and glucagon suppression
in patients with type 1 diabetes who have normal fasting glucose. Diabetes 2002;51:951-957
25 Holst JJ, Gromada J. Role of incretin hormones in the regulation of insulin secretion in diabetic and nondiabetic
humans. Am J Physiol Endocrinol Metab 2004;287:E199-206
, 百拇医药
26 Han G, Li XJ, Tian HM, Tong NW, Ouyang Q, Yin P. Study on gastric motility and its relevant factors in types 2 diabetes.
Huaxi Yike Daxue Xuebao 2001;32:296-299
27 Holzer P, Holzer-Petsche U. Tachykinin receptors in the gut:physiological and pathological implications.
Curr Opin Pharmacol 2001;1:583-590
28 Ji M, Lin L, Zhao ZQ. Substance p of plasma and enteric tissues in diabetes rats.
, 百拇医药
Nanjing Yike Daxue Xuebao(Ziran Kexueban) 2002;22:291-292
29 Zou YQ, Wang RJ, Lai TS, Li ZM. Experimental study of Zhishi Xiaopi pill on functional dyspepsia.
Zhongyao Xinyao Yu Linchuang Yaoli 2001;12:221-223
30 Wang CF, Yang DZ, WeiYQ, Xun QY. Study on effect of fructus aurantii immaturus on myoectric activity of
gastrointestinal tract in rats. Dongnan Daxue Xuebao(Yixueban) 2001;20:153-154
31 Zhu JZ, Zhang J, Xu QZ, Zhang ZJ, Leng ER, Chen DF. Mechanism of atractylodes macrocephala enhancing
gastrointestinal motility. Zhongguo Linchuang Yaoxue Zazhi 2001;10:365-368
编辑 张海宁, http://www.100md.com
杭州市卫生局资助项目, No. 2002B013
通讯作者:黄妙珍, 310015, 浙江省杭州市温州路126号, 杭州市第二人民医院. hmzhope@sina.com
电话: 0571-88303653
收稿日期: 2005-01-25 接受日期: 2005-02-26
摘要
目的:探讨枳实消痞颗粒对糖尿病大鼠胃肠激素的影响.
, http://www.100md.com
方法:SD雄性大鼠60只，随机选9只为正常组，不造模，其余大鼠禁食不禁水16 h，用四氧嘧啶进行腹腔注射，72 h后大鼠血糖大于11.1 mmol/L的为糖尿病模型成立.糖尿病大鼠随机分枳实消痞颗粒治疗组、多潘力酮混悬液对照组、模型组，各17只.每日相应灌枳实消痞颗粒溶液、多潘力酮混悬液和蒸馏水.实验4 wk后测定各大鼠血浆和胃组织中胃泌素、胃动素、胰高糖素、P物质.
结果:模型组、治疗组大鼠血浆胃泌素水平均低于正常组大鼠(t= 3.03，P= 0.01;t = 2.29，P= 0.04).模型组大鼠血浆胃动素水平低于正常组大鼠(t= 2.17，P= 0.04).治疗组、对照组大鼠胃动素水平均上升(t= 2.26，P= 0.04;t = 2.66，P= 0.02).模型组、对照组大鼠胰高糖素水平均高于正常组(t= 2.41，P= 0.03;t = 2.61，P= 0.02).P物质在各大鼠中表达无显著差异.模型组大鼠胃组织中胃泌素、胃动素水平比正常偏低，而枳实消痞颗粒能显著提高他们的水平(t= 2.43，P = 0.03;t = 2.25，P = 0.04).与模型组相比，对照组胃动素水平也显著上升(t= 2.22，P = 0.04).在大鼠胃组织中没有测出胰高糖素和P物质.
, 百拇医药
结论:枳实消痞颗粒能通过提高糖尿病大鼠血浆胃动素水平、胃组织中胃泌素和胃动素水平而改善其胃肠功能.
黄妙珍. 枳实消痞颗粒对糖尿病大鼠血浆和胃组织中胃肠激素的影响. 世界华人消化杂志 2005;13(6):790-7931 Domschke S, Bloom SR, Adrian TE, Lux G, Domschke W. Chronic pancreatitis and diabetes mellitus:plasma and
gastroduodenal mucosal profiles of regulatory peptides(gastrin, motilin, secretin, cholecystokinin, gastric inhibitory
polypeptide, somatostatin, VIP, substance P, pancreatic polypeptide, glucagon, enteroglucagon, neurotensin).
, http://www.100md.com
Hepatogastroenterology 1988;35:229-237
2 Pohle T, Domschke W. Gastric function measurements in drug development. Br J Clin Pharmacol 2003;56:156-164
3 Inui H, Yasuno R, Takenoshita M, Ohnishi Y, Sakamoto M, Matsuzaki J, Yamaji R, Miyatake K, Yamatodani A, Nakano Y.
Increases in gastric histidine decarboxylase activity and plasma gastrin level in streptozotocin-induced type 1 diabetic
, 百拇医药
rats. J Nutr Sci Vitaminol(Tokyo) 2000;46:144-148
4 Migdalis L, Thomaides T, Chairopoulos C, Kalogeropoulou C, Charalabides J, Mantzara F. Changes of gastric emptying
rate and gastrin levels are early indicators of autonomic neuropathy in type II diabetic patients. Clin Auton Res
2001;11:259-263
5 Rong SL, Li TG, Fang HM, Zheng YP. Effect of Chinese herbs for cool-moistening and freeing collaterals on serum
, http://www.100md.com
gastrin and surface electrogastrogram in patients of diabetes mellitus with gastroparesis.
Zhongguo Zhongxiyi Jiehe Zazhi 2004;24:976-978
6 El-Salhy M. Gastrointestinal transit in an animal model of human diabetes type 2: relationship to gut neuroendocrine
peptide contents. Ups J Med Sci 2002;107:101-110
7 Rooman I, Bouwens L. Combined gastrin and epidermal growth factor treatment induces islet regeneration and restores
, http://www.100md.com
normoglycaemia in C57Bl6/J mice treated with alloxan. Diabetologia 2004;47:259-265
8 Xu S, Yang Q, Cheng XF, Zhu JH, Xu JL. Effect of Lewei Fruid retention on gastrin and motilin in experimental model
of Spleen-deficiency rats. Zhongguo Yiyao Xuebao 2004;19:20-22
9 Xiao W, Liu J, Liu LY. Effect of jianpi wenshen decoction on serum gastrin, plasma motilin and somatostatin in patients
, http://www.100md.com
of diabetic diarrhea. Zhongguo Zhongxiyi Jiehe Zazhi 2002;22:587-589
10 Nilsson BI, Svenberg T, Tollstrom T, Hellstrom PM, Samuelson K, Schnell PO. Relationship between interdigestive
gallbladder emptying, plasma motilin and migrating motor complex in man. Acta Physiol Scand 1990;139:55-61
11 Zhou L, Wang LJ, Yuan B, Wang L. Effect of motilin on gastric smooth contraction induced by interstitial cells of
, http://www.100md.com
Cajal. Zhonghua Yixue Zazhi 2003;83:1422-1427
12 Huang J, Zhou H, Mahavadi S, Sriwai W, Lyall V, Murthy KS. Signaling pathways mediating gastrointestinal smooth
muscle contraction and MLC20 phosphorylation by motilin receptors. Am J Physiol Gastrointest Liver Physiol
2005;288:G23-31
13 Li H, Li GC. Changes of plasma MOT and antroduodenal SP expression in rats with functional dyspepsia and effect
, 百拇医药
of Qingzhishu. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:12-15
14 Liu SL, Mei GQ, Zhao YQ, Sun JQ, Wan XG, Zhang ML. Effect of Shugan Hewei decoction on motilin and gastrin in
blood of functional dyspepsia rats. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2004;12:198-200
15 Bai CC, Ma C, Li JY. Effect of Hewei decotion in motilin and gastrin with functional dyspepsia.
, http://www.100md.com
Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:373-374
16 Mao BY, Huang GF. Study of Jianpi Liqi medicine on gastrointestinal function and plasma motilin with dyspepsia.
Xin Zhongyi 2003;35:31-33
17 Xu SJ, Tang CH, Li XL, Dai Y. Effect of Yiqi Zengye granule on serum gastrin and plasma motilin in experimental model
of Spleen-deficiency diarrhea. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi 2003;11:9-11
, http://www.100md.com
18 Guo YQ, Yao SK, Fan HY, Tian YX, Huang SY, Feng L. Effect of Qingre Huashi detoction on serum gastrin, motilin,TNF-a1 and IL-10 in the dyspepsia patients with spleen-stomach damp-heat syndrome. Zhongguo Zhongyi Jichu
Yixue Zazhi 2004;10:63
19 Yuan J, Liu SY. Fasting serum motilin level and clinical meanings in diabetes. Dongnan Daxue Xuebao(Yixueban)
2003;22:262263
, 百拇医药
20 El-Salhy M, Spangeus A. Gastric emptying in animal models of human diabetes:correlation to blood glucose level and
gut neuroendocrine peptide content. Ups J Med Sci 2002;107:89-99
21 Kawagishi T, Nishizawa Y, Okuno Y, Sekiya K, Morii H. Effect of cisapride on gastric emptying of indigestible solids
and plasma motilin concentration in diabetic autonomic neuropathy. Am J Gastroenterol 1993;88:933-938
, 百拇医药
22 Peeters TL, Muls E, Janssens J, Urbain JL, Bex M, Van Cutsem E, Depoortere I, De Roo M, Vantrappen G, Bouillon R.
Effect of motilin on gastric emptying in patients with diabetic gastroparesis. Gastroenterology 1992;102:97-101
23 Vazeou A, Papadopoulou A, Papadimitriou A, Kitsou E, Stathatos M, Bartsocas CS. Autonomic neuropathy and
gastrointestinal motility disorders in children and adolescents with type 1 diabetes mellitus. J Pediatr Gastroenterol
, 百拇医药
Nutr 2004;38:61-65
24 Greenbaum CJ, Prigeon RL, D'Alessio DA. Impaired beta-cell function, incretin effect, and glucagon suppression
in patients with type 1 diabetes who have normal fasting glucose. Diabetes 2002;51:951-957
25 Holst JJ, Gromada J. Role of incretin hormones in the regulation of insulin secretion in diabetic and nondiabetic
humans. Am J Physiol Endocrinol Metab 2004;287:E199-206
, 百拇医药
26 Han G, Li XJ, Tian HM, Tong NW, Ouyang Q, Yin P. Study on gastric motility and its relevant factors in types 2 diabetes.
Huaxi Yike Daxue Xuebao 2001;32:296-299
27 Holzer P, Holzer-Petsche U. Tachykinin receptors in the gut:physiological and pathological implications.
Curr Opin Pharmacol 2001;1:583-590
28 Ji M, Lin L, Zhao ZQ. Substance p of plasma and enteric tissues in diabetes rats.
, 百拇医药
Nanjing Yike Daxue Xuebao(Ziran Kexueban) 2002;22:291-292
29 Zou YQ, Wang RJ, Lai TS, Li ZM. Experimental study of Zhishi Xiaopi pill on functional dyspepsia.
Zhongyao Xinyao Yu Linchuang Yaoli 2001;12:221-223
30 Wang CF, Yang DZ, WeiYQ, Xun QY. Study on effect of fructus aurantii immaturus on myoectric activity of
gastrointestinal tract in rats. Dongnan Daxue Xuebao(Yixueban) 2001;20:153-154
31 Zhu JZ, Zhang J, Xu QZ, Zhang ZJ, Leng ER, Chen DF. Mechanism of atractylodes macrocephala enhancing
gastrointestinal motility. Zhongguo Linchuang Yaoxue Zazhi 2001;10:365-368
编辑 张海宁, http://www.100md.com
