进展期胃癌HSP90a、CD44v6表达与其临床生物学行为关系的研究
费建东, 薛军, 河北北方学院附属第一医院普外科 河北省张家口市 075029
张林西, 金春亭, 薄爱华, 武欣, 张三明, 河北北方学院病理学教研室 河北省张家口市 075029
戴杰,首都医科大学教务处 北京市 100054
通讯作者:费建东, 075029, 河北省张家口市, 河北北方学院附属第一医院普外科.
电话: 0313-8043542
收稿日期: 2004-09-23 接受日期: 2004-10-20
, http://www.100md.com
摘要
目的:探讨HSP90a、CD44v6在进展期胃癌中的表达特点及其生物学行为，为进展期胃癌的临床治疗提供理论依据.
方法:对93例进展期胃癌及其56例淋巴结转移癌中HSP90a、CD44v6的表达，应用免疫组化方法进行检测.
结果:HSP90a在胃癌及淋巴结转移癌中的阳性率分别为54.84%(51/93)、66.07%(37/56).HSP90a在胃癌中表达分别与癌细胞浸润深度、淋巴结转移及TNM分期有关(P<0.05);CD44v6在胃癌及淋巴结转移癌中的阳性率分别为50.54%(47/93)、73.21%(41/56)，二者之间的差异显著(P<0.01).CD44v6在胃癌中的表达分别与癌细胞分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05).
, http://www.100md.com
结论:进展期胃癌中HSP90a、CD44v6阳性表达与其临床生物学行为尤其是淋巴结转移密切相关，患者可能预后较差，应采取积极的综合治疗措施.
费建东, 薛军, 张林西, 戴杰, 金春亭, 薄爱华, 武欣, 张三明. 进展期胃癌HSP90a、CD44v6表达与其临床生物学行为关系的研究. 世界华人消化杂志 2005;13(6):793-796
Sakate Y, Yashiro M, Tanaka H, Sunami T, Kosaka K, Hirakawa K. Usefulness of inhibiting the lymph node metastasis
in human gastric carcinoma by B7-1 gene transfection. J Surg Res 2004;122:89-95
, http://www.100md.com
3 Rodriguez Santiago JM, Munoz E, Marti M, Quintana S, Veloso E, Marco C. Metastatic lymph node ratio as a
prognostic factor in gastric cancer. Eur J Surg Oncol 2005;31:59-66
4 Lazaris AC, Theodoropoulos GE, Davaris PS, Panoussopoulos D, Nakopoulou L, Kittas C, Golematis BC. Heat shock
protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer. Dis Colon Rectum
, http://www.100md.com
1995;38:739-745
5 Lazaris AC, Theodoropoulos GE, Aroni K, Saetta A, Davaris PS. Immunohistochemical expression of C-myc oncogene,heat shock protein 70 and HLA-DR molecules in malignant cutaneous melanoma. Virchows Arch 1995;426:461-467
6 Volm M, Koomagi R, Mattern J, Stammler G. Heat shock(hsp70)and resistance proteins in non-small cell lung
carcinomas. Cancer Lett 1995;95:195-200
, 百拇医药
7 陈江, 张振玉, 祝金泉, 王崇文, 谢勇, 黄德强.CD44V6在食管癌中表达及临床意义. 华人消化杂志
1998;6:534
8 肖承志, 戴益民, 余宏宇, 王建军, 倪灿荣. CD44v6及nm23-H1表达与肝细胞癌侵袭转移.
华人消化杂志 1998;6:1036-1038
9 丁志杰, 单吉贤, 都姝妍. 直肠癌组织CD44v6,DNA含量的联合检测及临床意义. 世界华人消化杂志
2003;11:1382-1384
10 孙喜文, 申宝忠, 石美森, 戴旭东.CD44v6基因表达与胃癌危险因素的关系. 世界华人消化杂志
, 百拇医药
2002;10:1129-1132
11 米建强, 张朝晖, 沈铭昌. 胃癌及癌前病变组织中CD44v6表达的意义. 世界华人消化杂志 2000;8:156-158
12 Allgayer H, Heiss MM, Schildberg FW. Prognostic factors in gastriccancer. Br J Surg 1997;84:1651-1664
13 左东升, 范婕, 商爱民, 戴洁, 薄爱华. 热休克蛋白90a在人胃癌与癌旁及淋巴结转移癌中表达的研究.
张家口医学院学报 2002;19:4-5
14 Ciocca DR, Clark GM, Tandon AK, Fuqua SA, Welch WJ, McGuire WL. Heat shock protein hsp70 in patients with
, 百拇医药
axillary lymph node-negative breast cancer:prognostic implications. J Natl Cancer Inst 1993;85:570-574
15 Pearl LH, Prodromou C. Structure, function, and mechanism of the Hsp90 molecular chaperone.
Adv Protein Chem 2001;59:157-186
16 Zuo DS, Dai J, Bo AH, Fan J, Xiao XY. Significance of expression of heat shock protein90alpha in human gastric
cancer. World J Gastroenterol 2003;9:2616-2618
, http://www.100md.com
17 Kapranos N, Kominea A, Konstantinopoulos PA, Savva S, Artelaris S, Vandoros G, Sotiropoulou-Bonikou G,Papavassiliou AG. Expression of the 27-kDa heat shock protein(HSP27)in gastric carcinomas and adjacent normal,metaplastic, and dysplastic gastric mucosa, and its prognostic significance. J Cancer Res Clin Oncol
2002;128:426-432
18 Maehara Y, Oki E, Abe T, Tokunaga E, Shibahara K, Kakeji Y, Sugimachi K. Overexpression of the heat shock protein
, 百拇医药
HSP70 family and p53 protein and prognosis for patients with gastric cancer. Oncology 2000;58:144-151
19 Screaton GR, Bell MV, Jackson DG, Cornelis FB, Gerth U, Bell JI. Genomic structure of DNA encoding the
lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sci USA
1992;89:12160-12164
20 Gunthert U, Hofmann M, Rudy W, Reber S, Zoller M, Haussmann I, Matzku S, Wenzel A, Ponta H, Herrlich P. A new
variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 1991;65:13-24
21 Washington K, Gottfried MR, Telen MJ. Expression of the cell adhesion molecule CD44 in gastric adenocarcinomas.
Hum Pathol 1994;25:1043-1049
编辑 张海宁, http://www.100md.com( 费建东,薛 军,张林西, 戴 杰, 金春亭,薄爱华,武 欣,张三明)
张林西, 金春亭, 薄爱华, 武欣, 张三明, 河北北方学院病理学教研室 河北省张家口市 075029
戴杰,首都医科大学教务处 北京市 100054
通讯作者:费建东, 075029, 河北省张家口市, 河北北方学院附属第一医院普外科.
电话: 0313-8043542
收稿日期: 2004-09-23 接受日期: 2004-10-20
, http://www.100md.com
摘要
目的:探讨HSP90a、CD44v6在进展期胃癌中的表达特点及其生物学行为，为进展期胃癌的临床治疗提供理论依据.
方法:对93例进展期胃癌及其56例淋巴结转移癌中HSP90a、CD44v6的表达，应用免疫组化方法进行检测.
结果:HSP90a在胃癌及淋巴结转移癌中的阳性率分别为54.84%(51/93)、66.07%(37/56).HSP90a在胃癌中表达分别与癌细胞浸润深度、淋巴结转移及TNM分期有关(P<0.05);CD44v6在胃癌及淋巴结转移癌中的阳性率分别为50.54%(47/93)、73.21%(41/56)，二者之间的差异显著(P<0.01).CD44v6在胃癌中的表达分别与癌细胞分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05).
, http://www.100md.com
结论:进展期胃癌中HSP90a、CD44v6阳性表达与其临床生物学行为尤其是淋巴结转移密切相关，患者可能预后较差，应采取积极的综合治疗措施.
费建东, 薛军, 张林西, 戴杰, 金春亭, 薄爱华, 武欣, 张三明. 进展期胃癌HSP90a、CD44v6表达与其临床生物学行为关系的研究. 世界华人消化杂志 2005;13(6):793-796
Sakate Y, Yashiro M, Tanaka H, Sunami T, Kosaka K, Hirakawa K. Usefulness of inhibiting the lymph node metastasis
in human gastric carcinoma by B7-1 gene transfection. J Surg Res 2004;122:89-95
, http://www.100md.com
3 Rodriguez Santiago JM, Munoz E, Marti M, Quintana S, Veloso E, Marco C. Metastatic lymph node ratio as a
prognostic factor in gastric cancer. Eur J Surg Oncol 2005;31:59-66
4 Lazaris AC, Theodoropoulos GE, Davaris PS, Panoussopoulos D, Nakopoulou L, Kittas C, Golematis BC. Heat shock
protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer. Dis Colon Rectum
, http://www.100md.com
1995;38:739-745
5 Lazaris AC, Theodoropoulos GE, Aroni K, Saetta A, Davaris PS. Immunohistochemical expression of C-myc oncogene,heat shock protein 70 and HLA-DR molecules in malignant cutaneous melanoma. Virchows Arch 1995;426:461-467
6 Volm M, Koomagi R, Mattern J, Stammler G. Heat shock(hsp70)and resistance proteins in non-small cell lung
carcinomas. Cancer Lett 1995;95:195-200
, 百拇医药
7 陈江, 张振玉, 祝金泉, 王崇文, 谢勇, 黄德强.CD44V6在食管癌中表达及临床意义. 华人消化杂志
1998;6:534
8 肖承志, 戴益民, 余宏宇, 王建军, 倪灿荣. CD44v6及nm23-H1表达与肝细胞癌侵袭转移.
华人消化杂志 1998;6:1036-1038
9 丁志杰, 单吉贤, 都姝妍. 直肠癌组织CD44v6,DNA含量的联合检测及临床意义. 世界华人消化杂志
2003;11:1382-1384
10 孙喜文, 申宝忠, 石美森, 戴旭东.CD44v6基因表达与胃癌危险因素的关系. 世界华人消化杂志
, 百拇医药
2002;10:1129-1132
11 米建强, 张朝晖, 沈铭昌. 胃癌及癌前病变组织中CD44v6表达的意义. 世界华人消化杂志 2000;8:156-158
12 Allgayer H, Heiss MM, Schildberg FW. Prognostic factors in gastriccancer. Br J Surg 1997;84:1651-1664
13 左东升, 范婕, 商爱民, 戴洁, 薄爱华. 热休克蛋白90a在人胃癌与癌旁及淋巴结转移癌中表达的研究.
张家口医学院学报 2002;19:4-5
14 Ciocca DR, Clark GM, Tandon AK, Fuqua SA, Welch WJ, McGuire WL. Heat shock protein hsp70 in patients with
, 百拇医药
axillary lymph node-negative breast cancer:prognostic implications. J Natl Cancer Inst 1993;85:570-574
15 Pearl LH, Prodromou C. Structure, function, and mechanism of the Hsp90 molecular chaperone.
Adv Protein Chem 2001;59:157-186
16 Zuo DS, Dai J, Bo AH, Fan J, Xiao XY. Significance of expression of heat shock protein90alpha in human gastric
cancer. World J Gastroenterol 2003;9:2616-2618
, http://www.100md.com
17 Kapranos N, Kominea A, Konstantinopoulos PA, Savva S, Artelaris S, Vandoros G, Sotiropoulou-Bonikou G,Papavassiliou AG. Expression of the 27-kDa heat shock protein(HSP27)in gastric carcinomas and adjacent normal,metaplastic, and dysplastic gastric mucosa, and its prognostic significance. J Cancer Res Clin Oncol
2002;128:426-432
18 Maehara Y, Oki E, Abe T, Tokunaga E, Shibahara K, Kakeji Y, Sugimachi K. Overexpression of the heat shock protein
, 百拇医药
HSP70 family and p53 protein and prognosis for patients with gastric cancer. Oncology 2000;58:144-151
19 Screaton GR, Bell MV, Jackson DG, Cornelis FB, Gerth U, Bell JI. Genomic structure of DNA encoding the
lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sci USA
1992;89:12160-12164
20 Gunthert U, Hofmann M, Rudy W, Reber S, Zoller M, Haussmann I, Matzku S, Wenzel A, Ponta H, Herrlich P. A new
variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 1991;65:13-24
21 Washington K, Gottfried MR, Telen MJ. Expression of the cell adhesion molecule CD44 in gastric adenocarcinomas.
Hum Pathol 1994;25:1043-1049
编辑 张海宁, http://www.100md.com( 费建东,薛 军,张林西, 戴 杰, 金春亭,薄爱华,武 欣,张三明)
