【摘要】 目的 探讨灵芝对微小残留白血病小鼠的治疗效果及作用机制。方法 采用微小残留白血病(MRL)小鼠模型，用药组灌胃灵芝0.4g/kg，每日1次，连续12或22天，观察小鼠生存期、白血病细胞浸润脏器(脾脏和骨髓)的病理变化，应用流式细胞仪检测白血病细胞凋亡及细胞周期改变，采用ELISA方法检测小鼠血清中TNF-α含量，同时测定外周血白细胞、淋巴细胞数及亚群、骨髓粒系祖细胞集落形成单位(CFU-G)能力，与MRL模型组和正常对照组相比较。结果 灵芝给药组小鼠生存期较MRL模型组明显延长(P<0.001)。给予灵芝12天后，浸润脾脏的白血病细胞凋亡明显增加、活跃的DNA合成被有效抑制，脾脏白血病细胞浸润减少，外周血肿瘤坏死因子(TNF-α)水平显著升高。而外周血白细胞和淋巴细胞数在治疗后第3、7天明显高于MRL模型组，骨髓CFU-G数明显增加，紊乱的T细胞亚群基本恢复，脾脏白髓数增加且白髓体积增大，提示小鼠体内免疫反应增强。结论 灵芝可通过诱导白血病细胞凋亡、促进造血和提高免疫功能，延缓残留白血病的复发，其机制与体内TNF-α水平显著升高、免疫功能增加密切相关。

    关键词 灵芝 微小残留白血病 细胞凋亡 肿瘤坏死因子 粒系集落形成单位 小鼠

    Effects and its mechanism of ganoderma lucidum on the treatment of minimal residual leukemia in mice

    Chen Honghong，Cheng Wenying，Shi Jianhui，et al.

    Institute of Radiation Medicine，Fudan University，Shanghai200032

    【Abstract】 Objective To investigate the effects of ganoderma lucidum on the treatment of minimal residual leukemia in mice and explore its mechanism.Methods Ganoderma lucidum were administered orally to MRL model mice daily at the dose of0.4g/kg for twelve and twenty-two consecutive days，respectively.The survival time in MRL mice and pathological changes of spleen and bone marrow immersed by leukemic cell were observed.The apoptosis and cell cycle of leukemic cell were determined with flow cytometry.TNF-αactivity was measured by ELISA method.colony-forming tests were performed in bone marrow cells.The white blood cells ......