【摘要】 目的 简化黄芩苷提取纯化工艺，建立胃肠康颗粒剂中黄芩苷的含量测定方法。方法 通过超声提取酸沉、调节水溶液碱酸度除杂、甲醇超声助溶重结晶纯化工艺，获得黄芩苷对照品;并通过薄层层析、紫外光谱、红外光谱对其定性，HPLC法定量:色谱柱为Symmetry十八烷基键合硅胶柱，4.6mm×75mm，HPLC Column(Waters公司);甲醇-水-磷酸(47:53:0.2)作流动相，检测波长278nm。结果 得到的黄芩苷对照品理化性质与标准品一致，含量达99.25%，得率4.12%。在0.058～0.58μg范围，样品含量与色谱峰面积线性关系良好(r=0.9950)，加样回收率为98.80%～102.80%。结论 含量测定方法简便、快速，可用于胃肠康颗粒剂和相关中药颗粒剂产品中黄芩苷的含量测定。超声提取工艺简单，在中药活性成分提取方面有良好的应用前景。

    关键词 黄芩苷 制备 胃肠康颗粒 HPLC法 含量测定

    Preparation of baicalin and content determination in weichangkang granula by HPLC

    Li Qinglu，He Haibin，Cai Biqiong，et al.

    College of Life Science，Fujian Agriculture and Forestry University，Fujian350002.

    【Abstract】 Objective To simplify the process of extraction and purification for baicalin in traditional Chinese medicine，and establish a determination method of the content of baicalin in weichangkang granula.Methods The baicalin was extracted from the powder of scutellaria baicalensis georgi in ethanol with ultrasonic，precipitated in acid，the precipitation was dissolved in water and precipitated by adjusting pH，the deposid was dissolved with ultrasonic and recrystallized in methanol ......