胰腺干细胞及其治疗糖尿病的研究进展
宋振顺, 安家泽, 中国人民解放军第四军医大学西京医院肝胆外科 陕西省西安市 710032
宋振顺,男, 1961-1-30生, 上海市人, 汉族, 1984年第四军医大学本科毕业, 教授, 主要从事肝胆外科疑难疾病的诊治及肝移植、胰腺干细胞的研究.
通讯作者:宋振顺, 710032, 陕西省西安市长乐西路15号, 中国人民解放军第四军医大学西京医院肝胆外科. zhenshunsong@yahoo.com.cn
电话: 029-83373564
收稿日期: 2005-03-12 接受日期: 2005-03-23
, 百拇医药
摘要
胰岛素产生细胞的缺陷或缺乏导致的Ⅰ型糖尿病是影响人类健康的重要疾病之一.最近细胞移植和组织工程的研究进展，使得糖尿病的细胞替代治疗成为可能，供体的缺乏和移植排斥反应制约了胰岛移植的广泛应用，胰腺干细胞将成为胰岛素产生细胞的潜在来源，随着干细胞领域研究的深人，胰腺干细胞分离及诱导分化技术的进步将有助于解决这一问题.
关键词:干细胞; 糖尿病
宋振顺, 安家泽. 胰腺干细胞及其治疗糖尿病的研究进展. 世界华人消化杂志 2005;13(9):1052-10541 Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV. Islet transplantation in
, 百拇医药
seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.
N Engl J Med 2000;343:230-238
2 Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R. Differentiation of embryonic stem cells to insulin-secreting
structures similar to pancreatic islets. Science 2001;292:1389-1394
3 Assady S, Maor G, Amit M, Itskovitz-Eldor J, Skorecki KL, Tzukerman M. Insulin production by human embryonic stem
, 百拇医药
cells. Diabetes 2001;50:1691-1697
4 Bonner-Weir S, Taneja M, Weir GC, Tatarkiewicz K, Song KH, Sharma A, O'Neil JJ. In vitro cultivation of human islets
from expanded ductal tissue. Proc Natl Acad Sci USA 2000;97:7999-8004
5 Ferber S, Halkin A, Cohen H, Ber I, Einav Y, Goldberg I, Barshack I, Seijffers R, Kopolovic J, Kaiser N, Karasik A.
Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates
, 百拇医药
streptozotocin-induced hyperglycemia. Nat Med 2000;6:568-572
6 Suzuki A, Zheng YW, Kaneko S, Onodera M, Fukao K, Nakauchi H, Taniguchi H. Clonal identification and characterization
of self-renewing pluripotent stem cells in the developing liver. J Cell Biol 2002;156:173-184
7 McKinnon CM, Docherty K. Pancreatic duodenal homeobox-1, PDX-1, a major regulator of beta cell identity and function.
, http://www.100md.com
Diabetologia 2001;44:1203-1214
8 Gmyr V, Kerr-Conte J, Belaich S, Vandewalle B, Leteurtre E, Vantyghem MC, Lecomte-Houcke M, Proye C, Lefebvre J,Pattou F. Adult human cytokeratin 19-positive cells reexpress insulin promoter factor 1 in vitro:further evidence for
pluripotent pancreatic stem cells in humans. Diabetes 2000;49:1671-1680
9 Wang R, Li J, Yashpal N. Phenotypic analysis of c-Kit expression in epithelial monolayers derived from postnatal rat
, 百拇医药
pancreatic islets. J Endocrinol 2004;182:113-122
10 Peters K, Panienka R, Li J, Kloppel G, Wang R. Expression of stem cell markers and transcription factors during the
remodeling of the rat pancreas after duct ligation. Virchows Arch 2005;446:56-63
11 Jensen J, Heller RS, Funder-Nielsen T, Pedersen EE, Lindsell C, Weinmaster G, Madsen OD, Serup P. Independent
, 百拇医药
development of pancreatic alpha- and beta-cells from neurogenin3-expressing precursors:a role for the notch pathway
in repression of premature differentiation. Diabetes 2000;49:163-176
12 Schmied BM, Ulrich A, Matsuzaki H, Ding X, Ricordi C, Weide L, Moyer MP, Batra SK, Adrian TE, Pour PM.
Transdifferen-tiation of human islet cells in a long-term culture. Pancreas 2001;23:157-171
, 百拇医药
13 宋振顺, 顾克菊. 成人胰腺干细胞转分化为胰岛的研究. 中华外科杂志 2002;40:807-810
14 Soria B. In-vitro differentiation of pancreatic beta-cells. Differentiation 2001;68:205-219
15 Ramiya VK, Maraist M, Arfors KE, Schatz DA, Peck AB, Cornelius JG. Reversal of insulin-dependent diabetes using
islets generated in vitro from pancreatic stem cells. Nat Med 2000;6:278-282
16 Garcia-Ocana A, Vasavada RC, Cebrian A, Reddy V, Takane KK, Lopez-Talavera JC, Stewart AF. Transgenic
, http://www.100md.com
overex-pression of hepatocyte growth factor in the beta-cell markedly improves islet function and islet transplant
outcomes in mice. Diabetes 2001;50:2752-2762
17 Cornelius JG, Tchernev V, Kao KJ, Peck AB. In vitro-generation of islets in long-term cultures of pluripotent stem
cells from adult mouse pancreas. Horm Metab Res 1997;29:271-277
18 Lechner A. Stem cells and regenerative medicine for the treatment of type 1 diabetes:the challenges lying ahead.
Pediatr Diabetes 2004;5:88-93
19 Blyszczuk P, Wobus AM. Stem cells and pancreatic differentiation in vitro. J Biotechnol 2004;113:3-13
编辑 张海宁, 百拇医药( 宋振顺,安家泽)
宋振顺,男, 1961-1-30生, 上海市人, 汉族, 1984年第四军医大学本科毕业, 教授, 主要从事肝胆外科疑难疾病的诊治及肝移植、胰腺干细胞的研究.
通讯作者:宋振顺, 710032, 陕西省西安市长乐西路15号, 中国人民解放军第四军医大学西京医院肝胆外科. zhenshunsong@yahoo.com.cn
电话: 029-83373564
收稿日期: 2005-03-12 接受日期: 2005-03-23
, 百拇医药
摘要
胰岛素产生细胞的缺陷或缺乏导致的Ⅰ型糖尿病是影响人类健康的重要疾病之一.最近细胞移植和组织工程的研究进展，使得糖尿病的细胞替代治疗成为可能，供体的缺乏和移植排斥反应制约了胰岛移植的广泛应用，胰腺干细胞将成为胰岛素产生细胞的潜在来源，随着干细胞领域研究的深人，胰腺干细胞分离及诱导分化技术的进步将有助于解决这一问题.
关键词:干细胞; 糖尿病
宋振顺, 安家泽. 胰腺干细胞及其治疗糖尿病的研究进展. 世界华人消化杂志 2005;13(9):1052-10541 Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV. Islet transplantation in
, 百拇医药
seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.
N Engl J Med 2000;343:230-238
2 Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R. Differentiation of embryonic stem cells to insulin-secreting
structures similar to pancreatic islets. Science 2001;292:1389-1394
3 Assady S, Maor G, Amit M, Itskovitz-Eldor J, Skorecki KL, Tzukerman M. Insulin production by human embryonic stem
, 百拇医药
cells. Diabetes 2001;50:1691-1697
4 Bonner-Weir S, Taneja M, Weir GC, Tatarkiewicz K, Song KH, Sharma A, O'Neil JJ. In vitro cultivation of human islets
from expanded ductal tissue. Proc Natl Acad Sci USA 2000;97:7999-8004
5 Ferber S, Halkin A, Cohen H, Ber I, Einav Y, Goldberg I, Barshack I, Seijffers R, Kopolovic J, Kaiser N, Karasik A.
Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates
, 百拇医药
streptozotocin-induced hyperglycemia. Nat Med 2000;6:568-572
6 Suzuki A, Zheng YW, Kaneko S, Onodera M, Fukao K, Nakauchi H, Taniguchi H. Clonal identification and characterization
of self-renewing pluripotent stem cells in the developing liver. J Cell Biol 2002;156:173-184
7 McKinnon CM, Docherty K. Pancreatic duodenal homeobox-1, PDX-1, a major regulator of beta cell identity and function.
, http://www.100md.com
Diabetologia 2001;44:1203-1214
8 Gmyr V, Kerr-Conte J, Belaich S, Vandewalle B, Leteurtre E, Vantyghem MC, Lecomte-Houcke M, Proye C, Lefebvre J,Pattou F. Adult human cytokeratin 19-positive cells reexpress insulin promoter factor 1 in vitro:further evidence for
pluripotent pancreatic stem cells in humans. Diabetes 2000;49:1671-1680
9 Wang R, Li J, Yashpal N. Phenotypic analysis of c-Kit expression in epithelial monolayers derived from postnatal rat
, 百拇医药
pancreatic islets. J Endocrinol 2004;182:113-122
10 Peters K, Panienka R, Li J, Kloppel G, Wang R. Expression of stem cell markers and transcription factors during the
remodeling of the rat pancreas after duct ligation. Virchows Arch 2005;446:56-63
11 Jensen J, Heller RS, Funder-Nielsen T, Pedersen EE, Lindsell C, Weinmaster G, Madsen OD, Serup P. Independent
, 百拇医药
development of pancreatic alpha- and beta-cells from neurogenin3-expressing precursors:a role for the notch pathway
in repression of premature differentiation. Diabetes 2000;49:163-176
12 Schmied BM, Ulrich A, Matsuzaki H, Ding X, Ricordi C, Weide L, Moyer MP, Batra SK, Adrian TE, Pour PM.
Transdifferen-tiation of human islet cells in a long-term culture. Pancreas 2001;23:157-171
, 百拇医药
13 宋振顺, 顾克菊. 成人胰腺干细胞转分化为胰岛的研究. 中华外科杂志 2002;40:807-810
14 Soria B. In-vitro differentiation of pancreatic beta-cells. Differentiation 2001;68:205-219
15 Ramiya VK, Maraist M, Arfors KE, Schatz DA, Peck AB, Cornelius JG. Reversal of insulin-dependent diabetes using
islets generated in vitro from pancreatic stem cells. Nat Med 2000;6:278-282
16 Garcia-Ocana A, Vasavada RC, Cebrian A, Reddy V, Takane KK, Lopez-Talavera JC, Stewart AF. Transgenic
, http://www.100md.com
overex-pression of hepatocyte growth factor in the beta-cell markedly improves islet function and islet transplant
outcomes in mice. Diabetes 2001;50:2752-2762
17 Cornelius JG, Tchernev V, Kao KJ, Peck AB. In vitro-generation of islets in long-term cultures of pluripotent stem
cells from adult mouse pancreas. Horm Metab Res 1997;29:271-277
18 Lechner A. Stem cells and regenerative medicine for the treatment of type 1 diabetes:the challenges lying ahead.
Pediatr Diabetes 2004;5:88-93
19 Blyszczuk P, Wobus AM. Stem cells and pancreatic differentiation in vitro. J Biotechnol 2004;113:3-13
编辑 张海宁, 百拇医药( 宋振顺,安家泽)
