水蓑衣提取物对CCl4诱导的小鼠急性肝损伤的保护作用
冯大明, 王双, 唐雅玲, 杨永宗, 朱刚直, 颜迪忠, 南华大学病理生理学教研室、分子生物学研究中心 湖南省衡阳市 421001
冯大明,女, 1951-01-25生, 湖南省湘潭市人, 汉族, 1975年毕业于衡阳医学院, 病理生理学副教授.
通讯作者:颜迪忠, 421001, 湖南省衡阳市, 南华大学病理生理学教研室、分子生物学研究中心. yzyanghy@163.com
电话: 0734-8281288 传真: 0734-8281288
收稿日期: 2005-01-17 接受日期: 2005-02-02
, 百拇医药
Protective effect of extraction from Hygrophilasalicifolia on mice acute hepatic injury
Da-Ming Feng, Shuang Wang, Ya-Lin Tang, Yong-Zong Yang, Gang-Zhi Zhu,Di-Zhong Yan
Da-Ming Feng,Shuang Wang, Ya-Lin Tang, Yong-Zong Yang, Gang-Zhi Zhu, Di-Zhong Yan,Department of Pathophysiology and Center of Molecular Biology Nanhua University, Hengyang 421001, Hunan Province, China
, 百拇医药
Correspondence to: Di-Zhong Yan, Center of Molecular Biology Nanhua University,Hengyang 421001, Hunan Province,China. yzyanghy@163.com
Received: 2005-01-17 Accepted: 2005-02-02
Abstract
AIM: To investigate the hepatoprotective effect of the extraction from Hygrophila salicifolia.
METHODS: Forty male Kunming mice were divided equally into the normal control group, the CCl4 injury group, the low-dose (45 g/kg) Hygrophila salicifolia group, thehigh-dose (90 g/kg) Hygrophila salicifolia group, and the Bifendate (150mg/kg) group. All the mice except for those in the normal control grouphad acute hepatic injury induced by carbon tetrachloride (CCl4)10 mL/kg injected intraperitoneally. The animals were killed 16 h afterCCl4administration. The activities of serum ALT and AST, as well as thepathological changes of the liver were determined in all groups.
, 百拇医药
RESULTS: In comparison to the normal control group,the activitiesof serum ALT and AST in mice with CCl4injury were increased significantly (P<0.01). The liver histology showed mild to serious necrosis and degeneration. Serum ALT and AST in both Hygrophila salicifolia groups were significantly lower than those in CCl4 injury group(ALT:1 485±755,1 211±528, 3 179±106; AST:2 045±293,2 052±386, 2 583±116,in low-dose, high-dose Hygrophila salicifolia, and CCl4 injury group, respectively, all P<0.01). The liver necrosis anddegeneration induced by CCl4were also improved significantly after Hygrophila salicifoliaadministration. The effect of high-dose Hygrophila salicifolia wassuperior to that of Bifendate.
, 百拇医药
CONCLUSION: The extraction from Hygrophila salicifolia can protectthe liver from damage induced by CCl4 in a dose-dependentmanner. The effect of high-dose Hygrophila salicifolia is better than thatof Bifendate.
Key Words: CCl4; Acute hepatic injury; Hygrophila salicifolia; Extraction; Mice
Feng DM, Wang S, Tang YL, Yang YZ, Zhu GZ, Yan DZ. Protective effect of extraction from Hygrophila salicifolia on mice acute hepatic injury. Shijie Huaren Xiaohua Zazhi 2005;13(9):1098-1101
, 百拇医药
摘要
目的:探讨水蓑衣提取物对肝脏的保护作用.
方法:40只雄性昆明种小鼠，随机分为对照组、CCl4损伤对照组、低剂量水蓑衣(45 g/kg)组、高剂量水蓑衣(90 g/kg)组和联苯双酯(150 mg/kg)组.除正常对照组外，其余动物均采用ip CCl4复制急性肝损伤小鼠模型，CCl4注射后16 h处死动物，测定各组小鼠血清ALT、AST的活性以及检查肝组织的病理改变，观察不同剂量水蓑衣对肝脏的保护作用.
结果:与正常对照组相比，CCl4损伤对照组动物血清ALT和AST活性显著升高(P<0.01).肝脏出现轻度、中度和重度坏死及变性，低剂量水蓑衣组、高剂量水蓑衣组血清ALT和AST明显低于CCl4损伤对照组(ALT:1 485±755，1 211±528 vs 3 179±106;AST:2 045±293，2 052±386 vs 2 583±116，P<0.01)，与联苯双酯组相比无显著差异.水蓑衣能明显减轻CCl4引起的肝脏结构损害，高剂量水蓑衣的作用尤为突出，其作用优于联苯双酯.
, 百拇医药
结论:水蓑衣呈剂量依赖性地减轻CCl4引起的肝损害，其作用优于联苯双酯.
关键词:四氯化碳; 急性肝损伤; 水蓑衣; 提取物; 小鼠
冯大明, 王双, 唐雅玲, 杨永宗, 朱刚直, 颜迪忠. 水蓑衣提取物对CCl4诱导的小鼠急性肝损伤的保护作用. 世界华人消化杂志 2005;13(9):1098-1101
: 正常; B，C: CCl4损伤; D，E: 低剂量水蓑衣; F，G: 高剂量水蓑衣;H，I: 联苯双酯.
3 讨论CCl4肝损伤动物模型是研究护肝药物最常用的病理模型之一.CCl4引起肝损伤的机制为CCl4在肝微粒体细胞色素P-450激活下生成三氯甲基自由基，这些自由基可与肝细胞内大分子发生共价结合，也可攻击膜不饱和脂质，引起脂质过氧化，使肝细胞损伤，转氨酶从受损的肝细胞内溢出，使血清中转氨酶活性升高[2-5].小鼠ipCCl4后ALT，AST值明显高于正常对照组，病理学检查证实有肝细胞碎片状坏死、亚大块坏死、灶性坏死伴有中性粒细胞和单核/巨噬细胞浸润，并出现不同程度的肝细胞变性，这些与文献[4，7]报道的大致相同.已知联苯双酯对多种肝损伤动物模型及乙肝患者的肝脏有保护作用[5-6]，我们以联苯双酯作为CCl4中毒护肝的阳性对照.研究结果表明，联苯双酯组血清ALT和AST活性显著低于CCl4组，但防护肝脏结构受损的作用不如水蓑衣显著，肝脏仍有亚大块坏死，较多灶性坏死及嗜酸性变.给予水蓑衣后，各剂量组ALT、AST值较CCl4对照组有明显降低，差异有显著性，并且明显减轻小鼠肝脏的病理损伤，其中高剂量水蓑衣较低剂量水蓑衣的作用更为显著，显示出一定的剂量依赖性，表明水蓑衣能够减轻CCl4对肝细胞的急性损害，对肝脏具有保护作用.而且其减轻CCl4所致肝脏结构损害方面优于联苯双酯.水蓑衣抗肝损伤的作用是否与抗脂质过氧化有联系，药物是促进毒性代谢产物的解毒，还是直接保护肝细胞免受损伤，有待于进一步研究.
, 百拇医药
4 参考文献1 杨成梓, 凌伟坚, 陈建斌. 两种仙桃草的本草考证. 中药材 2003;26:818-819
2 张建平, 魏红山, 刘顺爱, 郭晶晶, 张黔英, 石小红, 张四平, 刘志英, 冯鑫, 吕洪波. 依那普利对CCl4
急性肝损伤大鼠抗氧化功能的影响. 世界华人消化杂志 2004;12:2638-2641
3 Liu SL, Degli Esposti S, Yao T, Diehl AM, Zern MA. Vitmin Etherapy of acute CCl4-induced hepatic injury in mice is
associated with inhibition of nuclear factor kappa B binding. Hepatology 1995;22:1474-1481
, 百拇医药
4 Jose SL, Daniel MF, Pablo MDT. Protective effect of carnosol on CCl4-induced acute liver damage in rats.
Europ J Gastroenterol Hepatol 2002;14:1001-1006
5 张学武, 赵莲芳, 全吉叔, 沈明花. 珍珠梅水提取物对四氯化碳所致大鼠急性肝损伤的保护作用.
中南药学 2003;1:207-209
6 崔速南, 汪明明, 范桂玲. 大剂量联苯双酯抗乙型肝炎病毒疗效的临床观察. 中华医学杂志 2002;82:538-540
7 徐玉芳, 黄向春, 温子龙, 罗左阳, 黄常青, 雷元金. 克肝胶囊对小鼠实验性急性肝损伤的影响.
中西医结合肝病杂志 2000;10:23-25
编辑 潘伯荣 审读 张海宁, 百拇医药( 冯大明,王 双,唐雅玲, 杨永宗,朱刚直,颜迪忠)
冯大明,女, 1951-01-25生, 湖南省湘潭市人, 汉族, 1975年毕业于衡阳医学院, 病理生理学副教授.
通讯作者:颜迪忠, 421001, 湖南省衡阳市, 南华大学病理生理学教研室、分子生物学研究中心. yzyanghy@163.com
电话: 0734-8281288 传真: 0734-8281288
收稿日期: 2005-01-17 接受日期: 2005-02-02
, 百拇医药
Protective effect of extraction from Hygrophilasalicifolia on mice acute hepatic injury
Da-Ming Feng, Shuang Wang, Ya-Lin Tang, Yong-Zong Yang, Gang-Zhi Zhu,Di-Zhong Yan
Da-Ming Feng,Shuang Wang, Ya-Lin Tang, Yong-Zong Yang, Gang-Zhi Zhu, Di-Zhong Yan,Department of Pathophysiology and Center of Molecular Biology Nanhua University, Hengyang 421001, Hunan Province, China
, 百拇医药
Correspondence to: Di-Zhong Yan, Center of Molecular Biology Nanhua University,Hengyang 421001, Hunan Province,China. yzyanghy@163.com
Received: 2005-01-17 Accepted: 2005-02-02
Abstract
AIM: To investigate the hepatoprotective effect of the extraction from Hygrophila salicifolia.
METHODS: Forty male Kunming mice were divided equally into the normal control group, the CCl4 injury group, the low-dose (45 g/kg) Hygrophila salicifolia group, thehigh-dose (90 g/kg) Hygrophila salicifolia group, and the Bifendate (150mg/kg) group. All the mice except for those in the normal control grouphad acute hepatic injury induced by carbon tetrachloride (CCl4)10 mL/kg injected intraperitoneally. The animals were killed 16 h afterCCl4administration. The activities of serum ALT and AST, as well as thepathological changes of the liver were determined in all groups.
, 百拇医药
RESULTS: In comparison to the normal control group,the activitiesof serum ALT and AST in mice with CCl4injury were increased significantly (P<0.01). The liver histology showed mild to serious necrosis and degeneration. Serum ALT and AST in both Hygrophila salicifolia groups were significantly lower than those in CCl4 injury group(ALT:1 485±755,1 211±528, 3 179±106; AST:2 045±293,2 052±386, 2 583±116,in low-dose, high-dose Hygrophila salicifolia, and CCl4 injury group, respectively, all P<0.01). The liver necrosis anddegeneration induced by CCl4were also improved significantly after Hygrophila salicifoliaadministration. The effect of high-dose Hygrophila salicifolia wassuperior to that of Bifendate.
, 百拇医药
CONCLUSION: The extraction from Hygrophila salicifolia can protectthe liver from damage induced by CCl4 in a dose-dependentmanner. The effect of high-dose Hygrophila salicifolia is better than thatof Bifendate.
Key Words: CCl4; Acute hepatic injury; Hygrophila salicifolia; Extraction; Mice
Feng DM, Wang S, Tang YL, Yang YZ, Zhu GZ, Yan DZ. Protective effect of extraction from Hygrophila salicifolia on mice acute hepatic injury. Shijie Huaren Xiaohua Zazhi 2005;13(9):1098-1101
, 百拇医药
摘要
目的:探讨水蓑衣提取物对肝脏的保护作用.
方法:40只雄性昆明种小鼠，随机分为对照组、CCl4损伤对照组、低剂量水蓑衣(45 g/kg)组、高剂量水蓑衣(90 g/kg)组和联苯双酯(150 mg/kg)组.除正常对照组外，其余动物均采用ip CCl4复制急性肝损伤小鼠模型，CCl4注射后16 h处死动物，测定各组小鼠血清ALT、AST的活性以及检查肝组织的病理改变，观察不同剂量水蓑衣对肝脏的保护作用.
结果:与正常对照组相比，CCl4损伤对照组动物血清ALT和AST活性显著升高(P<0.01).肝脏出现轻度、中度和重度坏死及变性，低剂量水蓑衣组、高剂量水蓑衣组血清ALT和AST明显低于CCl4损伤对照组(ALT:1 485±755，1 211±528 vs 3 179±106;AST:2 045±293，2 052±386 vs 2 583±116，P<0.01)，与联苯双酯组相比无显著差异.水蓑衣能明显减轻CCl4引起的肝脏结构损害，高剂量水蓑衣的作用尤为突出，其作用优于联苯双酯.
, 百拇医药
结论:水蓑衣呈剂量依赖性地减轻CCl4引起的肝损害，其作用优于联苯双酯.
关键词:四氯化碳; 急性肝损伤; 水蓑衣; 提取物; 小鼠
冯大明, 王双, 唐雅玲, 杨永宗, 朱刚直, 颜迪忠. 水蓑衣提取物对CCl4诱导的小鼠急性肝损伤的保护作用. 世界华人消化杂志 2005;13(9):1098-1101
: 正常; B，C: CCl4损伤; D，E: 低剂量水蓑衣; F，G: 高剂量水蓑衣;H，I: 联苯双酯.
3 讨论CCl4肝损伤动物模型是研究护肝药物最常用的病理模型之一.CCl4引起肝损伤的机制为CCl4在肝微粒体细胞色素P-450激活下生成三氯甲基自由基，这些自由基可与肝细胞内大分子发生共价结合，也可攻击膜不饱和脂质，引起脂质过氧化，使肝细胞损伤，转氨酶从受损的肝细胞内溢出，使血清中转氨酶活性升高[2-5].小鼠ipCCl4后ALT，AST值明显高于正常对照组，病理学检查证实有肝细胞碎片状坏死、亚大块坏死、灶性坏死伴有中性粒细胞和单核/巨噬细胞浸润，并出现不同程度的肝细胞变性，这些与文献[4，7]报道的大致相同.已知联苯双酯对多种肝损伤动物模型及乙肝患者的肝脏有保护作用[5-6]，我们以联苯双酯作为CCl4中毒护肝的阳性对照.研究结果表明，联苯双酯组血清ALT和AST活性显著低于CCl4组，但防护肝脏结构受损的作用不如水蓑衣显著，肝脏仍有亚大块坏死，较多灶性坏死及嗜酸性变.给予水蓑衣后，各剂量组ALT、AST值较CCl4对照组有明显降低，差异有显著性，并且明显减轻小鼠肝脏的病理损伤，其中高剂量水蓑衣较低剂量水蓑衣的作用更为显著，显示出一定的剂量依赖性，表明水蓑衣能够减轻CCl4对肝细胞的急性损害，对肝脏具有保护作用.而且其减轻CCl4所致肝脏结构损害方面优于联苯双酯.水蓑衣抗肝损伤的作用是否与抗脂质过氧化有联系，药物是促进毒性代谢产物的解毒，还是直接保护肝细胞免受损伤，有待于进一步研究.
, 百拇医药
4 参考文献1 杨成梓, 凌伟坚, 陈建斌. 两种仙桃草的本草考证. 中药材 2003;26:818-819
2 张建平, 魏红山, 刘顺爱, 郭晶晶, 张黔英, 石小红, 张四平, 刘志英, 冯鑫, 吕洪波. 依那普利对CCl4
急性肝损伤大鼠抗氧化功能的影响. 世界华人消化杂志 2004;12:2638-2641
3 Liu SL, Degli Esposti S, Yao T, Diehl AM, Zern MA. Vitmin Etherapy of acute CCl4-induced hepatic injury in mice is
associated with inhibition of nuclear factor kappa B binding. Hepatology 1995;22:1474-1481
, 百拇医药
4 Jose SL, Daniel MF, Pablo MDT. Protective effect of carnosol on CCl4-induced acute liver damage in rats.
Europ J Gastroenterol Hepatol 2002;14:1001-1006
5 张学武, 赵莲芳, 全吉叔, 沈明花. 珍珠梅水提取物对四氯化碳所致大鼠急性肝损伤的保护作用.
中南药学 2003;1:207-209
6 崔速南, 汪明明, 范桂玲. 大剂量联苯双酯抗乙型肝炎病毒疗效的临床观察. 中华医学杂志 2002;82:538-540
7 徐玉芳, 黄向春, 温子龙, 罗左阳, 黄常青, 雷元金. 克肝胶囊对小鼠实验性急性肝损伤的影响.
中西医结合肝病杂志 2000;10:23-25
编辑 潘伯荣 审读 张海宁, 百拇医药( 冯大明,王 双,唐雅玲, 杨永宗,朱刚直,颜迪忠)
