【摘要】 目的 研究哮喘豚鼠肺内不同密度嗜酸细胞(Eos)凋亡与bcl-2表达的关系及药物对它们的影响。方法 应用地塞米松(DM)、雷公藤甲素(TP)及氨茶碱(AM)干预哮喘豚鼠，分离哮喘豚鼠支气管肺泡灌洗液中不同密度Eos，以TUNEL法检测细胞凋亡，以原位杂交检测bcl-2mRNA表达。结果 哮喘组Eos凋亡率较正常对照组明显降低(P<0.01)，应用DM、TP、AM后均显著增加(P<0.01)。正常豚鼠Eos可检测到bcl-2mRNA表达，不同密度Eos表达差异无显著性(P>0.05)。哮喘组bcl-2mRNA明显增加，应用DM及TP后其表达明显减少，而用AM后bcl-2表达变化不明显。结论 凋亡调节的缺失是导致组织及血中Eos增多的重要原因，bcl-2参与了哮喘Eos凋亡的调节。DM、TP及AM可促进哮喘肺组织中的Eos细胞凋亡，bcl-2可能是DM及TP调节Eos细胞凋亡的重要途径之一。

    its changes after drug treatment

    Li Zhikui，Liu Gang，Wang Changzheng，et al.

    Department of Respiratory Medicine，Xi Jing Hospital，Fourth Military Medical University，Xi’an710032.

    【Abstract】 Objective To study the ralation between the apoptosis and bcl-2expression in eosinophils of bronchoalveolar lavage fluid(BALF)in asthmatic guinea pig and its changes after drug treatment.Methods Dexamˉethasone(DM)，triptolide(TP)and aminophylline(AM)have been used in guinea pig of allergic asthma.Apoptosis were detected by TdT-mediated dUTP nick end labeling，bcl-2mRNA expression were measured by hybridization.Reˉsults Apoptotic eosinophils in asthma group were significantly less than normal control group(P<0.01) ......