【摘要】 目的 探讨P16基因甲基化与大肠癌发生发展的关系，研究从血清中筛选，诊断大肠癌的可行性。方法 采用MsP法检测36例大肠癌组织、7例大肠腺瘤瘤体组织及对应患者血清中P16基因甲基化状况，同时分析与临床病理特征的关系，10例健康志愿者血清标本作为对照组。结果 36例癌组织中检出P16基因甲基化15例，阳性率41.7%;癌组织标本中检出P16基因甲基化的15例对应患者血清标本中有9例也检测到同样改变，阳性率60%(9/15)。其余21例癌组织标本未检出P16基因甲基化其对应患者血清、7例腺瘤组织标本及对应患者血清，10例健康志愿者血清标本中均未检测到P16基因甲基化畸变。结论 P16基因甲基化与大肠癌的发生发展有关，血清中检测其变化可望成为监测患者预后的无创性诊断方法。

    关键词 P16基因 甲基化 大肠癌 血清

    Detection of hypermethylation of P16gene and

    its application in diagnosis of colorectal cancer

    Chen Jianzhi，Zhang Zhen shu，Zhou Jingxu，et al.

    Department of Digestive Disease，the188th Hospital of PLA，Chaozhou521000.

    【Abstrac】 Objective To search for the relationship between hypermethylation of a5’CpG island of P16gene and colorectal cancer(CRC)，and study the feasibility of detecting methylation of P16gene in DNA extracted from the serum of patients with colorectal cancer.Methods Tumor and sermn DNA were extracted from36patients with colˉorectal cancer(stages A-D)，and from7patients with polyous.Then P16methylation was detected using methylation-specific PCR with10healthy subjects as controls.Results P16gene methylation was found in44.7%(15of36)of colorectal cancel-tissue.Among15cases with aberrant methylation in the tumor tissues ......