【摘要】 目的 探讨米非司酮对子宫肌瘤培养细胞的表皮生长因子受体及表皮生长因子受体基因表达的影响。 方法 (1)用0.1、1.0、10μmol/L的米非司酮和0μmol/L的米非司酮(对照组)干预培养的子宫肌瘤细胞。(2)用免疫组化SABC法及半定量RT-PCR法分别测定米非司酮干预4天后的各组子宫肌瘤细胞上的EGFR水平及EGFRmRNA表达量。 结果 EGFR及EGFRmRNA的表达在分泌期高于增生期，米非司酮干预后子宫肌瘤细胞EGFR及EGFRmRNA在1.0μmol/L和10μmol/L组表达量明显下降，与对照组和0.1μmol/L组之间差异有显著性(P<0.05)。而1.0μmol/L组和10μmol/L组之间无明显差异(P>0.05)。对照组和0.1μmol/L组之间差异无显著性(P>0.05)。 结论 (1)子宫肌瘤中EGFR及EGFRmRNA在分泌期高于增生期。(2)米非司酮可通过抑制EGFRmRNA的基因表达，下调EGFR。这可能是米非司酮治疗子宫肌瘤的另一重要机制。(3)米非司酮抑制子宫肌瘤的EGFR及EGFRmRNA表达与剂量相关。

    【关键词】 米非司酮 子宫肌瘤 细胞培养 表皮生长因子受体

    Effects of mifepristone on ER，PR，epidermal growth factor receptor

    and EGFRmRNA in cultured human leiomyoma cells

    Ren Mulan，Wei Aimin，Chen Xiling，et al.

    The Affiliated ZhongDa Hospital of Southeast University，Nanjing210009.

    【Abstract】 Objective To study the effects of mifepristone on ppidermal growth factor-receptor and EGFRmRNA in cultured human leiomyoma cells in vitro.Methods (1)Cultured leiomyoma cells were treated with0.1，1.0，10μmol/L mifepristone and0μmol/L mifepristone(control group).(2)The expression of EGFR and EGFR mR-NA in leiomyoma after4days'treatment of mifepristone were determined respectively by immunochemistry method of Strepavidin-Biotin-Peroxidase Complex(SABC)and semiquantitative RT-PCR.Results EGFR and EGFRmRNA levels in leiomyoma cells are higher in secretory phase than in proliferative phase.EGFR and EGFRmRNA levels in leiomyoma cells of1.0 ......