【摘要】 目的 探讨虎杖与黄芪组方抗人疱疹病毒(HSV)时，其有效成分白藜芦醇与总黄酮之间的相互作用对药效的影响。 方法 以阿昔洛韦(ACV)为阳性对照，采用空斑减数实验，用中效作用原理对虎杖白藜芦醇与黄芪总黄酮合用抗Ⅱ型人疱疹病毒HSV-2333株的药效进行了分析。 结果 在Hep-2细胞系统中，ACV对HSV-2333株增殖抑制的ED50为10.85μg/ml。当虎杖白藜芦醇与总黄酮以1(Dm)白藜芦醇/1(Dm)总黄酮的比例合用时，对HSV-2333株直接杀灭、感染阻断、增殖抑制的ED50依次为0.91μg/ml，1.12μg/ml，0.77μg/ml;合用指数(Combination Index，TI)均<1.0，P<0.0005，说明这一合用比药效最强，协同作用最明显。 结论 ACV对HSV-2333株增殖抑制的治疗指数(Treatment Indices，TIS)为100。1(Dm)白藜芦醇/1(Dm)总黄酮对HSV-2333株直接杀灭、感染阻断、增殖抑制的TIs为125.5，102.0，148.3，为最佳合用比。

    关键词 虎杖白藜芦醇 黄芪总黄酮 Ⅱ型人疱疹病毒333株

    The effect analysis of antiherpes simplex virus type-2strain333with combination of resveratol of Rhizoma polygoni cuspidati and total flavonoids of Astragalus membranaceus

    Wang Zhijie，Huang Qishan

    Virus Research Institute，Medical College of Wuhan University，Wuhan430071.

    【Abstract】 Objective In order to exploit the influence which was produced by the interaction between the ef-fective components resveratol and total flavonoids on efficiency of antiHSV-2strain333，when an antiHSV medicinal Chinese herbs preparations was made up using Rhizoma polygoni cuspidati and Astragalus membranaceus ......