【摘要】 目的 建立获得性多药耐药(MDR)基因表达小鼠模型，探讨临床早期发现化疗耐药、系统检测耐药程度和逆转耐药的方法。 方法 模拟临床联合化疗，给予小鼠腹水型S180细胞反复刺激，诱导MDR表达并定量检测表达量和S180细胞凋亡指标，应用维拉帕米(verapamil，VPL)调节耐药性的逆转。 结果 实验组小鼠自化疗后1周MDR表达逐渐增高，自第5周后显著性增加(P<0.01);而S180细胞Fas因子表达及凋亡率则随化疗时间的延长而逐渐下降，MDR表达显著性增加时，S180细胞凋亡率等则显著性降低(P<0.05)。 结论 本方法建立的S180细胞多药耐药小鼠模型有助于获得在体研究MDR表达的经验，应用VPL能调节耐药性的逆转。

    关键词 动物模型 多药耐药基因 细胞凋亡 化疗 调节剂

    The study of obtained MDR gene expression of mouse-ascites S180cell line and adjusting of chemotherapy resistance

    Zhu Tongyu，Yin Geping，Chen Ming，et al.

    Department of Gynecology and Obstetrics，Jinan Military Command General Hospital，Jinan250031.

    【Abstract】 Objective To establish the mouse-ascites S180-line model with obtained multidrug resistant(MDR)gene xpression in order to discover the clinical method for indicating and systematic examining the chemotherapy esistance.Methods The mouse ascites S180cells were repeatedly stimulated by imitating clinically combined cheotherapy to induce the MDR xpression.Then the cellular P 170 contents and apoptotic parameters of the S180cells were assayed.Verapamil(VPL)was used to adjust the obtained chemotherapy-resistance.Results The P 170 contents of mouse in test group began to increase since the irstweek ......