【摘要】 目的 探讨SARS患者体内SARS病毒抗体产生的规律及比较IFA和ELISA法的特点。 方法 采用间接免疫荧光技术(IFA)和酶联免疫吸附试验(ELISA)对43例SARS患者双份血清及10例正常人双份血清的SARS病毒IgM/IgG抗体进行检测。 结果 43例患者中新近感染SARS病毒40例(93.02%)，3例未检出其感染。发病10天内检出率较低，发病10天后检出率较高。阳性率随发病时间而上升。在SARS病毒IgG的检测中，ELISA法较IFA法敏感。 结论 IFA法和ELISA法均适合于SARS发病10天后实验室辅助诊断方法，ELISA法较IFA法敏感。

    关键词 SARS病毒 酶联免疫吸附试验 间接免疫荧光技术 IgM IgG

    The comparison between ELISA and IFA methods for determining antibodies of IgG and IgM in patients with SARS

    Li Suifen，Tan Yizhou，Tang Yangbo，et al.

    Guangzhou Eighth People's Hospital，Guangzhou510060.

    【Abstract】 Objective To study and discuss the rules of the SARS virus antibody production in patients with severe acute respiratory syndrome(SARS)，compare the characteristics of IFA and ELISA.Methods The SARS virus IgM/IgG antibodies of double serums of43patients with SARS and10normal controls were detected by IFA and ELISA.Results 40out of43(93.02%)patients with SARS infected SARS virus recently and other three patients can't be detected the antibody.The antibody detection rate that with in10days of the disease taking bad is lower than that after10days ......