美国科学家开发新型非典疫苗动物实验获成功
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美国科学家新近开发了一种SARS实验疫苗。这种疫苗能激发老鼠体内的免疫反应，并大大减少一些老鼠肺部的SARS病毒数量。
开发出这种新型基因型疫苗的美国国家过敏症和传染病研究所的研究人员指出，必须还要做更多的实验，才能确定这种疫苗是否能运用于人类。
据悉，疫苗通常利用已被杀死或者毒性减弱的病毒制成。这种疫苗在注入人体后，免疫系统就能认出和攻击“活”着的病毒。但不同的是，这种新型SARS疫苗则是由DNA质粒上的一片遗传物质制成，它能自动跟踪锁定病毒细胞外层的一种特异蛋白质，从而激发动物体内的免疫系统反击病毒的入侵。
在六周的实验期中，科学家们向15只老鼠体内注入了这种基因疫苗的两种变体，它们所含遗传物质的含量有所不同。但结果显示，两种都有效，只是其中一种看起来效果更好一些。
另外一些科学家们认为，这项成果是鼓舞人心的，但也并不能夸大其意义。他们说，至今还没有DNA疫苗能有效对付哪一种病毒型疾病。
, 百拇医药
这项研究成果已发表在了4月1日出版的《自然》(Nature)杂志上。
Nature. 2004 Apr 1;428(6982):561-4.
A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice.
Zhi-Yong Yang, Wing-Pui Kong, Yue Huang, Anjeanette Roberts, Brian R. Murphy, Kanta Subbarao & Gary J. Nabel.
Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC-3005, 40 Convent Drive, Bethesda, Maryland 20892-3005, USA. Laboratory of Infectious Diseases, NIAID, National Institutes of Health, MSC-8007, 50 South Drive, Bethesda, Maryland 20892-8007, USA.
, 百拇医药
Public health measures have successfully identified and contained outbreaks of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), but concerns remain over the possibility of future recurrences. Finding a vaccine for this virus therefore remains a high priority. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Alternative forms of S were analysed by DNA immunization. These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay. Moreover, antibody responses in mice vaccinated with an expression vector encoding a form of S that includes its transmembrane domain elicited neutralizing antibodies. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Gene-based vaccination for the SARS-CoV elicits effective immune responses that generate protective immunity in an animal model., http://www.100md.com
开发出这种新型基因型疫苗的美国国家过敏症和传染病研究所的研究人员指出，必须还要做更多的实验，才能确定这种疫苗是否能运用于人类。
据悉，疫苗通常利用已被杀死或者毒性减弱的病毒制成。这种疫苗在注入人体后，免疫系统就能认出和攻击“活”着的病毒。但不同的是，这种新型SARS疫苗则是由DNA质粒上的一片遗传物质制成，它能自动跟踪锁定病毒细胞外层的一种特异蛋白质，从而激发动物体内的免疫系统反击病毒的入侵。
在六周的实验期中，科学家们向15只老鼠体内注入了这种基因疫苗的两种变体，它们所含遗传物质的含量有所不同。但结果显示，两种都有效，只是其中一种看起来效果更好一些。
另外一些科学家们认为，这项成果是鼓舞人心的，但也并不能夸大其意义。他们说，至今还没有DNA疫苗能有效对付哪一种病毒型疾病。
, 百拇医药
这项研究成果已发表在了4月1日出版的《自然》(Nature)杂志上。
Nature. 2004 Apr 1;428(6982):561-4.
A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice.
Zhi-Yong Yang, Wing-Pui Kong, Yue Huang, Anjeanette Roberts, Brian R. Murphy, Kanta Subbarao & Gary J. Nabel.
Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC-3005, 40 Convent Drive, Bethesda, Maryland 20892-3005, USA. Laboratory of Infectious Diseases, NIAID, National Institutes of Health, MSC-8007, 50 South Drive, Bethesda, Maryland 20892-8007, USA.
, 百拇医药
Public health measures have successfully identified and contained outbreaks of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), but concerns remain over the possibility of future recurrences. Finding a vaccine for this virus therefore remains a high priority. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Alternative forms of S were analysed by DNA immunization. These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay. Moreover, antibody responses in mice vaccinated with an expression vector encoding a form of S that includes its transmembrane domain elicited neutralizing antibodies. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Gene-based vaccination for the SARS-CoV elicits effective immune responses that generate protective immunity in an animal model., http://www.100md.com