食管上皮永生化细胞系研究进展
李健, 齐义军, 王立东, 郑州大学医学院癌症实验室
河南省郑州市 450052
李健, 郑州大学第二附属医院消化内科 河南省郑州市 450003
通讯作者: 王立东, 450052, 河南省郑州市, 郑州大学医学院癌症研究室. ldwang@zzu.edu.cn
电话: 0371-66658335
收稿日期: 2004-11-09 接受日期: 2005-02-26
摘要
, http://www.100md.com
建立合适的模型系统是研究食管癌癌变过程的重要课题.食管永生化细胞株较难建立，他既具有正常细胞的特征，而且已经处于持续增生状态，易于转化，对研究食管癌变过程是一种良好工具模型.本文就近年来有关食管上皮永生化细胞的研究进展作一综述.
李健, 齐义军, 王立东. 食管上皮永生化细胞系研究进展. 世界华人消化杂志 2005;13(11):1317-1321
参考文献
1 Bocchetta M, Carbone M. Epidemiology and molecular pathology atcrossroads to establish causation:
molecular mechanisms of malignanttransformation. Oncogene 2004;23:6484-6491
, 百拇医药
2 Stampfer MR, Yaswen P. Human epithelial cell immortalization as astep in carcinogenesis. Cancer Lett 2003;
194:199-208
3 Lundberg AS, Hahn WC, Gupta P, Weinberg RA. Genes involved insenescence and immortalization. Curr Opin Cell
Biol 2000;12:705-709
4 Gil J, Kerai P, Lleonart M, Bernard D, Cigudosa JC, Peters G,Carnero A, Beach D. Immortalization of primary
, http://www.100md.com
human prostate epithelial cells by c-Myc. CancerRes 2005;65:2179-2185
5 Lawson T, Ouellette M, Kolar C, Hollingsworth M. Culture andimmortalization of pancreatic ductal epithelial cells.
Methods Mol Med 2004;103:113-122
6 Kroll J. Molecular chaperones and the process of cellularimmortalization in vitro. Biogerontology 2002;3:183-185
7 Newbold RF. The significance of telomerase activation and cellularimmortalization in human cancer.
, 百拇医药
Mutagenesis 2002;17:539-550
8 Wang H, Spillare EA, Wang QS, Sabourin CLK, Stoner GD.p53-independent down-regulation of cyclin D1 and p21Waf1
in the process of immortalization of humanesophageal epithelial cells. Int J Oncol 1998;12:325-328
9 Vaziri H, Benchimol S. Alternative pathways for the extension ofcellular life span: inactivation of p53/pRb and
, 百拇医药 expression of telomerase. Oncogene 1999;18:7676-7680
10 Li H, Cao Y, Berndt MC, Funder JW, Liu JP. Molecular interactionsbetween telomerase and the tumor suppressor
protein p53 in vitro. Oncogene 1999;18:6785-6794
11 Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA,Klingelhutz AJ. Both Rb/p16INK4a inactivation and
telomerase activity are required toimmortalize human epithelial cells. Nature 1998;396:84-88
, 百拇医药
12 Stoner GD, Kaighn ME, Reddel RR, Resau JH, Bowman D, Naito Z,Matsukura N, You M, Galati AJ, Harris CC.
Establishment and characterization ofSV40 T-antigen immortalized human esophageal epithelial cells. Cancer
Res 1991;51:365-371
13 Meeker AK, DeMarzo AM. Recent advances in telomere biology: implications for humancancer. Curr Opin
Oncol 2004;16:32-38
, 百拇医药
14 Munger K, Howley PM. Human papillomavirusimmortalization and transformation functions. Virus Res 2002;
89:213-228
15 Steenbergen RD, de Wilde J, Wilting SM, Brink AA, Snijders PJ,Meijer CJ. HPV-mediated transformation of the
anogenital tract. J Clin Virol 2005;32(Suppl):25-33
16 Si HX, Tsao SW, Poon CS, Wang LD, Wong YC, Cheung AL. Viral load ofHPV in esophageal squamous cell carcinoma.
, 百拇医药
Int J Cancer 2003;103:496-500
17 Toh Y, Kuwano H, Tanaka S, Baba K, Matsuda H, Sugimachi K, Mori R.Detection of human papillomavirus DNA
in esophageal carcinoma in Japan bypolymerase chain reaction. Cancer 1992;70:2234-2238
18 Furihata M, Ohtsuki Y, Ogoshi S, Takahashi A, Tamiya T, Ogata T.Prognostic significance of human
papillomavirus genomes (type-16, -18)and aberrant expression of p53 protein in human esophageal cancer. IntJ
, 百拇医药
Cancer 1993;54:226-230
19 Shen ZY, Cen S, Shen J, Cai W, Xu J, Teng Z, Hu Z, Zeng Y. Study ofimmortalization and malignant transformation
of human embryonic esophagealepithelial cells induced by HPV18E6E7. J Cancer Res Clin Oncol 2000;126:589-594
20 Shen ZY, Xu LY, Chen XH, Cai WJ, Shen J, Chen JY, Huang TH, Zeng Y.The genetic events of
HPV-immortalized esophagealepithelium cells. Int J Mol Med 2001;8:537-542
, 百拇医药
21 Shen ZY, Xu LY, Li EM, Cai WJ, Chen MH, Shen J, Zeng Y. Telomereand telomerase in the initial stage of
immortalization of esophagealepithelial cell. World J Gastroenterol 2002;8:357-362
22 Shen ZY, Xu LY, Li EM, Cai WJ, Shen J, Chen MH, Cen S, Tsao SW,Zeng Y. The multistage process of carcinogenesis
in human esophageal epithelial cellsinduced by human papillomavirus. Oncol Rep 2004;11:647-654
, 百拇医药
23 Sashiyama H, Shino Y, Sakao S, Shimada H, Kobayashi S, Ochiai T,Shirasawa H. Alteration of integrin expression
relates to malignant progression ofhuman papillomavirus-immortalized esophageal keratinocytes. Cancer
Lett 2002;177:21-28
24 Hofmann MC, Braydich-Stolle L, Dettin L, Johnson E, Dym M.Immortalization of mouse germ line stem cells. Stem
Cells 2005;23:200-210
25 Rahman NA, Huhtaniemi IT. Testicular cell lines. Mol CellEndocrinol 2004;228:53-65
编辑 张海宁, 百拇医药( 李 健, 齐义军, 王立东)
河南省郑州市 450052
李健, 郑州大学第二附属医院消化内科 河南省郑州市 450003
通讯作者: 王立东, 450052, 河南省郑州市, 郑州大学医学院癌症研究室. ldwang@zzu.edu.cn
电话: 0371-66658335
收稿日期: 2004-11-09 接受日期: 2005-02-26
摘要
, http://www.100md.com
建立合适的模型系统是研究食管癌癌变过程的重要课题.食管永生化细胞株较难建立，他既具有正常细胞的特征，而且已经处于持续增生状态，易于转化，对研究食管癌变过程是一种良好工具模型.本文就近年来有关食管上皮永生化细胞的研究进展作一综述.
李健, 齐义军, 王立东. 食管上皮永生化细胞系研究进展. 世界华人消化杂志 2005;13(11):1317-1321
参考文献
1 Bocchetta M, Carbone M. Epidemiology and molecular pathology atcrossroads to establish causation:
molecular mechanisms of malignanttransformation. Oncogene 2004;23:6484-6491
, 百拇医药
2 Stampfer MR, Yaswen P. Human epithelial cell immortalization as astep in carcinogenesis. Cancer Lett 2003;
194:199-208
3 Lundberg AS, Hahn WC, Gupta P, Weinberg RA. Genes involved insenescence and immortalization. Curr Opin Cell
Biol 2000;12:705-709
4 Gil J, Kerai P, Lleonart M, Bernard D, Cigudosa JC, Peters G,Carnero A, Beach D. Immortalization of primary
, http://www.100md.com
human prostate epithelial cells by c-Myc. CancerRes 2005;65:2179-2185
5 Lawson T, Ouellette M, Kolar C, Hollingsworth M. Culture andimmortalization of pancreatic ductal epithelial cells.
Methods Mol Med 2004;103:113-122
6 Kroll J. Molecular chaperones and the process of cellularimmortalization in vitro. Biogerontology 2002;3:183-185
7 Newbold RF. The significance of telomerase activation and cellularimmortalization in human cancer.
, 百拇医药
Mutagenesis 2002;17:539-550
8 Wang H, Spillare EA, Wang QS, Sabourin CLK, Stoner GD.p53-independent down-regulation of cyclin D1 and p21Waf1
in the process of immortalization of humanesophageal epithelial cells. Int J Oncol 1998;12:325-328
9 Vaziri H, Benchimol S. Alternative pathways for the extension ofcellular life span: inactivation of p53/pRb and
, 百拇医药 expression of telomerase. Oncogene 1999;18:7676-7680
10 Li H, Cao Y, Berndt MC, Funder JW, Liu JP. Molecular interactionsbetween telomerase and the tumor suppressor
protein p53 in vitro. Oncogene 1999;18:6785-6794
11 Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA,Klingelhutz AJ. Both Rb/p16INK4a inactivation and
telomerase activity are required toimmortalize human epithelial cells. Nature 1998;396:84-88
, 百拇医药
12 Stoner GD, Kaighn ME, Reddel RR, Resau JH, Bowman D, Naito Z,Matsukura N, You M, Galati AJ, Harris CC.
Establishment and characterization ofSV40 T-antigen immortalized human esophageal epithelial cells. Cancer
Res 1991;51:365-371
13 Meeker AK, DeMarzo AM. Recent advances in telomere biology: implications for humancancer. Curr Opin
Oncol 2004;16:32-38
, 百拇医药
14 Munger K, Howley PM. Human papillomavirusimmortalization and transformation functions. Virus Res 2002;
89:213-228
15 Steenbergen RD, de Wilde J, Wilting SM, Brink AA, Snijders PJ,Meijer CJ. HPV-mediated transformation of the
anogenital tract. J Clin Virol 2005;32(Suppl):25-33
16 Si HX, Tsao SW, Poon CS, Wang LD, Wong YC, Cheung AL. Viral load ofHPV in esophageal squamous cell carcinoma.
, 百拇医药
Int J Cancer 2003;103:496-500
17 Toh Y, Kuwano H, Tanaka S, Baba K, Matsuda H, Sugimachi K, Mori R.Detection of human papillomavirus DNA
in esophageal carcinoma in Japan bypolymerase chain reaction. Cancer 1992;70:2234-2238
18 Furihata M, Ohtsuki Y, Ogoshi S, Takahashi A, Tamiya T, Ogata T.Prognostic significance of human
papillomavirus genomes (type-16, -18)and aberrant expression of p53 protein in human esophageal cancer. IntJ
, 百拇医药
Cancer 1993;54:226-230
19 Shen ZY, Cen S, Shen J, Cai W, Xu J, Teng Z, Hu Z, Zeng Y. Study ofimmortalization and malignant transformation
of human embryonic esophagealepithelial cells induced by HPV18E6E7. J Cancer Res Clin Oncol 2000;126:589-594
20 Shen ZY, Xu LY, Chen XH, Cai WJ, Shen J, Chen JY, Huang TH, Zeng Y.The genetic events of
HPV-immortalized esophagealepithelium cells. Int J Mol Med 2001;8:537-542
, 百拇医药
21 Shen ZY, Xu LY, Li EM, Cai WJ, Chen MH, Shen J, Zeng Y. Telomereand telomerase in the initial stage of
immortalization of esophagealepithelial cell. World J Gastroenterol 2002;8:357-362
22 Shen ZY, Xu LY, Li EM, Cai WJ, Shen J, Chen MH, Cen S, Tsao SW,Zeng Y. The multistage process of carcinogenesis
in human esophageal epithelial cellsinduced by human papillomavirus. Oncol Rep 2004;11:647-654
, 百拇医药
23 Sashiyama H, Shino Y, Sakao S, Shimada H, Kobayashi S, Ochiai T,Shirasawa H. Alteration of integrin expression
relates to malignant progression ofhuman papillomavirus-immortalized esophageal keratinocytes. Cancer
Lett 2002;177:21-28
24 Hofmann MC, Braydich-Stolle L, Dettin L, Johnson E, Dym M.Immortalization of mouse germ line stem cells. Stem
Cells 2005;23:200-210
25 Rahman NA, Huhtaniemi IT. Testicular cell lines. Mol CellEndocrinol 2004;228:53-65
编辑 张海宁, 百拇医药( 李 健, 齐义军, 王立东)
