【摘要】 目的 探讨庆大霉素在肾近端小管的吸收途径。 方法 将大鼠随机分为实验组和对照组，每组又分注射5min组、注射20min组。利用活体肾小管显微穿刺技术，将 3 H-庆大霉素或庆大霉素分别注入实验组和对照组近端小管腔内，常规制作光镜、电镜放射自显影切片，显微镜观察、摄影并进行图像分析。 结果 光镜放射自显影显示: 3 H-庆大霉素注入近端小管5min后，放射自显影银颗粒分布于近端小管顶部;20min后，近端小管中银颗粒比5min时明显增多(P<0.01)，中部也可见银颗粒。远端小管、细段及集合小管等均未见特异颗粒;对照组近端小管无银颗粒存在。电镜自显影显示:灌注5min组银颗粒集中分布在内吞小泡、大泡和致密顶端小管中，而溶酶体偶见;20min组溶酶体中银颗粒明显增多。 结论 庆大霉素可能与近端小管上皮顶部上的某种受体———膜蛋白结合，通过内吞小泡、大泡和致密顶端小管传送至溶酶体并可积聚于此。

    【关键词】 庆大霉素 肾脏 近端小管 放射自显影

    The morphological studies of absorption way of gentamicin in proximal tubule

    Mu Changzheng，Wang Xiaomei，Tao Shiying，et al.

    Department of Histology and Enbryology，Jinzhou Medical College，Jinzhou121001.

    【Abstract】 Objective To investigate the absorption way of gentamicin in proximal tubule.Methods Rats were randomly divided into two groups:the experimental group received3H-gentamicin which was injected into proxi-mal tubule and the control group received gentamicin.According to the time after the operation ......